Abstract
Rare conjugational progeny formed by crossing each of five Hfr strains with a recA-F- strain have been characterized. Selection was made for a proximal Hfr marker, taking strict precautions to prevent transfer of recA+ to the zygotes. Most of the progeny were found to be F' strains containing deletion mutant plasmids. With two exceptions, these mutant plasmids have lost all of the tra genes, which are required to confer conjugational donor ability upon a host. In addition, all but the exceptional mutant plasmids were found to be very poorly transmissible from transient heterozygotes which also contain a wild-type F' plasmid. The poor transmissibility is a cis-dominant transfer-defective phenotype which may result from deletion of all or part of the origin of transfer replication (ori), or of a gene determining a cis-acting protein. The two exceptional mutant plasmids may carry short deletions of some of the tra genes or polar tra mutations. The remaining progeny were nonmutant F' strains and F- strains. The frequency with which the F- strains were recovered permits us to estimate that the maximum amount of recombination possible in a recA56 zygote is 10(-6) that of a recA+ zygote.
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Selected References
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- Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Achtman M., Willetts N., Clark A. J. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli. J Bacteriol. 1972 Jun;110(3):831–842. doi: 10.1128/jb.110.3.831-842.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anthony W. M., Deonier R. C., Lee H. J., Hu S., Otsubo E., Davidson N., Broda P. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IX. Note on the deletion mutant of F, F delta(33-43). J Mol Biol. 1974 Nov 15;89(4):647–650. doi: 10.1016/0022-2836(74)90041-2. [DOI] [PubMed] [Google Scholar]
- BECKWITH J. R., PARDEE A. B., AUSTRIAN R., JACOB F. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol. 1962 Dec;5:618–634. doi: 10.1016/s0022-2836(62)80090-4. [DOI] [PubMed] [Google Scholar]
- Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Britton J. R., Haselkorn R. Permeability lesions in male Escherichia coli infected with bacteriophage T7. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2222–2226. doi: 10.1073/pnas.72.6.2222. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Broda P., Meacock P., Achtman M. Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12. Mol Gen Genet. 1972;116(4):336–347. doi: 10.1007/BF00270090. [DOI] [PubMed] [Google Scholar]
- CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chakrabarti S. L., Gorini L. A link between streptomycin and rifampicin mutation. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2084–2087. doi: 10.1073/pnas.72.6.2084. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark A. J., Maas W. K., Low B. Production of a merodiploid strain from a double male strain of E. coli K12. Mol Gen Genet. 1969;105(1):1–15. doi: 10.1007/BF00750309. [DOI] [PubMed] [Google Scholar]
- Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
- DEWITT S. K., ADELBERG E. A. Transduction of the attached sex factor of Escherichia coli. J Bacteriol. 1962 Mar;83:673–678. doi: 10.1128/jb.83.3.673-678.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finnegan D. J., Willetts N. S. Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Mol Gen Genet. 1971;111(3):256–264. doi: 10.1007/BF00433110. [DOI] [PubMed] [Google Scholar]
- Finnegan D., Willetts N. The site of action of the F transfer inhibitor. Mol Gen Genet. 1973 Dec 31;127(4):307–316. doi: 10.1007/BF00267101. [DOI] [PubMed] [Google Scholar]
- Francke B., Ray D. S. Cis-limited action of the gene-A product of bacteriophage phiX174 and the essential bacterial site (E. coli-electron microscopy-cis-acting protein-specifically-nicked RF). Proc Natl Acad Sci U S A. 1972 Feb;69(2):475–479. doi: 10.1073/pnas.69.2.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HORIUCHI K., ADELBERG E. A. GROWTH OF MALE-SPECIFIC BACTERIOPHAGE IN PROTEUS MIRABILIS HARBORING F-GENOTES DERIVED FROM ESCHERICHIA COLI. J Bacteriol. 1965 May;89:1231–1236. doi: 10.1128/jb.89.5.1231-1236.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Helinski D. R., Clewell D. B. Circular DNA. Annu Rev Biochem. 1971;40:899–942. doi: 10.1146/annurev.bi.40.070171.004343. [DOI] [PubMed] [Google Scholar]
- Henry T. J., Knippers R. Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549–1553. doi: 10.1073/pnas.71.4.1549. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ippen-Ihler K., Achtman M., Willetts N. Deletion map of the Escherichia coli K-12 sex factor F: the order of eleven transfer cistrons. J Bacteriol. 1972 Jun;110(3):857–863. doi: 10.1128/jb.110.3.857-863.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACOB F., CAMPBELL A. Sur le système de répression assurant l'immunité chez les bactéries lysogenes. C R Hebd Seances Acad Sci. 1959 Jun 1;248(22):3219–3221. [PubMed] [Google Scholar]
- Kupersztoch-Portnoy Y. M., Lovett M. A., Helinski D. R. Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K. Biochemistry. 1974 Dec 31;13(27):5484–5490. doi: 10.1021/bi00724a005. [DOI] [PubMed] [Google Scholar]
- LOW B., WOOD T. H. A QUICK AND EFFICIENT METHOD FOR INTERRUPTION OF BACTERIAL CONJUGATION. Genet Res. 1965 Jul;6:300–303. doi: 10.1017/s001667230000416x. [DOI] [PubMed] [Google Scholar]
- Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morrison T. G., Malamy M. H. T7 translational control mechanisms and their inhibiton by F factors. Nat New Biol. 1971 May 12;231(19):37–41. doi: 10.1038/newbio231037a0. [DOI] [PubMed] [Google Scholar]
- Novick R. P., Brodsky R. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus. J Mol Biol. 1972 Jul 21;68(2):285–302. doi: 10.1016/0022-2836(72)90214-8. [DOI] [PubMed] [Google Scholar]
- Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Otsubo E., Nishimura Y., Hirota Y. Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis. Genetics. 1970 Feb;64(2):173–188. doi: 10.1093/genetics/64.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Otsubo E. Transfer-defective mutants of sex factors in Escherichia coli. II. Deletion mutants of an F-prime and deletion mapping of cistrons involved in genetic transfer. Genetics. 1970 Feb;64(2):189–197. doi: 10.1093/genetics/64.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Press R., Glansdorff N., Miner P., De Vries J., Kadner R., Maas W. K. Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome. Proc Natl Acad Sci U S A. 1971 Apr;68(4):795–798. doi: 10.1073/pnas.68.4.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
- Skurray R. A., Reeves P. F factor-mediated immunity to lethal zygosis in Escherichia coli K-12. J Bacteriol. 1974 Jan;117(1):100–106. doi: 10.1128/jb.117.1.100-106.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Summers W. C., Jakes K. Phage T7 lysozyme mRNA transcription and translation in vivo and in vitro. Biochem Biophys Res Commun. 1971 Oct 15;45(2):315–320. doi: 10.1016/0006-291x(71)90820-5. [DOI] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N. S. Characterization of the F transfer cistron, traL. Genet Res. 1973 Apr;21(2):205–213. doi: 10.1017/s0016672300013379. [DOI] [PubMed] [Google Scholar]
- Willetts N. S., Clark A. J., Low B. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol. 1969 Jan;97(1):244–249. doi: 10.1128/jb.97.1.244-249.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N. S. Location of the origin of transfer of the sex factor F. J Bacteriol. 1972 Nov;112(2):773–778. doi: 10.1128/jb.112.2.773-778.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N. S., Mount D. W. Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA. J Bacteriol. 1969 Nov;100(2):923–934. doi: 10.1128/jb.100.2.923-934.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N., Achtman M. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. J Bacteriol. 1972 Jun;110(3):843–851. doi: 10.1128/jb.110.3.843-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N., Bastarrachea F. Genetic and physicochemical characterization of Escherichia coli strains carrying fused F' elements derived from KLF1 and F57. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1481–1485. doi: 10.1073/pnas.69.6.1481. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N. Mapping loci for surface exclusion and incompatibility on the F factor of Escherichia coli K-12. J Bacteriol. 1974 Jun;118(3):778–782. doi: 10.1128/jb.118.3.778-782.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N. The genetics of transmissible plasmids. Annu Rev Genet. 1972;6:257–268. doi: 10.1146/annurev.ge.06.120172.001353. [DOI] [PubMed] [Google Scholar]
- Wood T. H. Effects of temperature, agitation, and donor strain on chromosome transfer in Escherichia coli K-12. J Bacteriol. 1968 Dec;96(6):2077–2084. doi: 10.1128/jb.96.6.2077-2084.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]