Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Jan;129(1):66–70. doi: 10.1128/jb.129.1.66-70.1977

Threonyl-transfer ribonucleic acid synthetase and the regulation of the threonine operon in Escherichia coli.

E J Johnson, G N Cohen, I Saint-Girons
PMCID: PMC234895  PMID: 318654

Abstract

Two threonine-requiring mutants with derepressed expression of the threonine operon were isolated from an Escherichia coli K-12 strain containing two copies of the thr operon. One of them carries a leaky mutation in ilvA (the structural gene for threonine deaminase), which creates an isoleucine limitation and therefore derepression of the thr operon. In the second mutant, the enzymes of the thr operon were not repressed by threonine plus isoleucine; the threonyl-transfer ribonucleic acid(tRNA) synthetase from this mutant shows an apparent Km for threonine 200-fold higher than that of the parental strain. The gene, called thrS, coding for threonyl-tRNA synthetase was located around 30 min on the E. coli map. The regulatory properties of this mutant imply the involvement of charged threonyl-tRNA or threonyl-tRNA synthetase in the regulation of the thr operon.

Full text

PDF
66

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boy E., Reinisch F., Richaud C., Patte J. C. Role of lysyl-tRNA in the regulation of lysine biosynthesis in Escherichia coli K12. Biochimie. 1976;58(1-2):213–218. doi: 10.1016/s0300-9084(76)80372-0. [DOI] [PubMed] [Google Scholar]
  2. Brickman E., Soll L., Beckwith J. Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. J Bacteriol. 1973 Nov;116(2):582–587. doi: 10.1128/jb.116.2.582-587.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FREUNDLICH M. Multivalent repression in the biosynthesis of threonine in Salmonella typhimurium and Escherichia coli. Biochem Biophys Res Commun. 1963 Feb 6;10:277–282. doi: 10.1016/0006-291x(63)90430-3. [DOI] [PubMed] [Google Scholar]
  4. Gardner J. F., Smith O. H. Operator-promoter functions in the threonine operon of Escherichia coli. J Bacteriol. 1975 Oct;124(1):161–166. doi: 10.1128/jb.124.1.161-166.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hiraga S., Ito K., Hamada K., Yura T. A new regulatory gene for the tryptophan operon of Escherichia coli. Biochem Biophys Res Commun. 1967 Mar 9;26(5):522–527. doi: 10.1016/0006-291x(67)90095-2. [DOI] [PubMed] [Google Scholar]
  6. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  7. Nass G., Poralla K., Zähner H. Effect of the antibiotic Borrelidin on the regulation of threonine biosynthetic enzymes in E. coli. Biochem Biophys Res Commun. 1969 Jan 6;34(1):84–91. doi: 10.1016/0006-291x(69)90532-4. [DOI] [PubMed] [Google Scholar]
  8. Nass G., Thomale J. Alteration of structure of level of threonyl-tRNA-synthetase in Borrelidin resistant mutants of E. coli. FEBS Lett. 1974 Feb 15;39(2):182–186. doi: 10.1016/0014-5793(74)80046-3. [DOI] [PubMed] [Google Scholar]
  9. PATTE J. C., LE BRAS G., LOVINY T., COHEN G. N. [Retro-inhibition and repression of the homoserine dehydrogenase of Escherichia coli]. Biochim Biophys Acta. 1963 Jan 8;67:16–30. doi: 10.1016/0006-3002(63)91793-1. [DOI] [PubMed] [Google Scholar]
  10. Paetz W., Nass G. Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12. Eur J Biochem. 1973 Jun;35(2):331–337. doi: 10.1111/j.1432-1033.1973.tb02843.x. [DOI] [PubMed] [Google Scholar]
  11. Roth J. R., Ames B. N. Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase. J Mol Biol. 1966 Dec 28;22(2):325–333. doi: 10.1016/0022-2836(66)90135-5. [DOI] [PubMed] [Google Scholar]
  12. Saint-Girons I., Margarita D. Operator-constitutive mutants in the threonine operon of Escherichia coli K-12. J Bacteriol. 1975 Dec;124(3):1137–1141. doi: 10.1128/jb.124.3.1137-1141.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Skarstedt M. T., Greer S. B. Threonine synthetase of Bacillus subtilis. The nature of an associated dehydratase activity. J Biol Chem. 1973 Feb 10;248(3):1032–1044. [PubMed] [Google Scholar]
  14. Szentirmai A., Umbarger H. E. Isoleucine and valine metabolism of Escherichia coli. XIV. Effect of thiaisoleucine. J Bacteriol. 1968 May;95(5):1666–1671. doi: 10.1128/jb.95.5.1666-1671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thèze J., Saint-Girons I. Threonine locus of Escherichia coli K-12: genetic structure and evidence for an operon. J Bacteriol. 1974 Jun;118(3):990–998. doi: 10.1128/jb.118.3.990-998.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WORMSER E. H., PARDEE A. B. Regulation of threonine biosynthesis in Escherichia coli. Arch Biochem Biophys. 1958 Dec;78(2):416–432. doi: 10.1016/0003-9861(58)90367-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES