Abstract
Cotransduction of two or even three plasmids was observed when a Staphylococcus aureus strain, carrying five distinct, compatible plasmids, was used as donor. An active host recombination system did not seem to be indispensable for plasmid cotransduction, since RecA+ and RecA- donors gave similar cotransduction frequencies. Analysis of plasmids carried by cotransductant clones demonstrated that a genetic interaction can take place between cotransduced plasmids, leading to new plasmids. Some of the properties of these new plasmids are discussed. Another set of experiments tested the ability of a cotransducible plasmid to allow a significant degree of multiplication of a temperature-sensitive plasmid at restrictive temperature. In an attempt to explain the results obtained, a working hypothesis suggesting a transient and reversible association of cotransducible plasmids is presented.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubnau E., Stocker B. A. Behavior of three colicine factors and an R (drug resistance) factor in Hfr crosses in Salmonella typhimurium. Genet Res. 1967 Jun;9(3):283–297. doi: 10.1017/s0016672300010582. [DOI] [PubMed] [Google Scholar]
- Grindley N. D., Humphreys G. O., Anderson E. S. Molecular studies of R factor compatibility groups. J Bacteriol. 1973 Jul;115(1):387–398. doi: 10.1128/jb.115.1.387-398.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grubb W. B., O'Reilly R. J. Joint transduction of separate extrachromosomal drug resistance determinants in Staphylococcus aureus E169. Biochem Biophys Res Commun. 1971 Feb 5;42(3):377–383. doi: 10.1016/0006-291x(71)90381-0. [DOI] [PubMed] [Google Scholar]
- Grubb W. B., O'Reilly R. J., May J. W. Segregation o co-transduced streptomycin and tetracycline resistance in Staphylococcus aureus. Genet Res. 1972 Aug;20(1):43–50. doi: 10.1017/s0016672300013574. [DOI] [PubMed] [Google Scholar]
- Helinski D. R. Plasmid determined resistance to antibiotics: molecular properties of R factors. Annu Rev Microbiol. 1973;27:437–470. doi: 10.1146/annurev.mi.27.100173.002253. [DOI] [PubMed] [Google Scholar]
- Iordănescu S., Greceanu V. Localisation du déterminant de la streptomycino- résistance chez une souche de Staphylococcus aureus. Arch Roum Pathol Exp Microbiol. 1971 Sep-Dec;30(3):463–471. [PubMed] [Google Scholar]
- Iordănescu S. Host controlled restriction mutants of Staphylococcus aureus. Arch Roum Pathol Exp Microbiol. 1975 Jan-Jun;34(1-2):55–58. [PubMed] [Google Scholar]
- Iordănescu S. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids. J Bacteriol. 1975 Nov;124(2):597–601. doi: 10.1128/jb.124.2.597-601.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kopecko D. J., Cohen S. N. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1373–1377. doi: 10.1073/pnas.72.4.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novick R. P., Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. doi: 10.1111/j.1749-6632.1971.tb30664.x. [DOI] [PubMed] [Google Scholar]
- Novick R. P., Roth C. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J Bacteriol. 1968 Apr;95(4):1335–1342. doi: 10.1128/jb.95.4.1335-1342.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novick R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967 Sep;33(1):155–166. doi: 10.1016/0042-6822(67)90105-5. [DOI] [PubMed] [Google Scholar]
- Ruby C., Novick R. P. Plasmid interactions in Staphylococcus aureus: nonadditivity of compatible plasmid DNA pools. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5031–5035. doi: 10.1073/pnas.72.12.5031. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stiffler P. W., Sweeney H. M., Cohen S. Co-transduction of plasmids mediating resistance to tetracycline and chloramphenicol in Staphylococcus aureus. J Bacteriol. 1974 Nov;120(2):934–934. doi: 10.1128/jb.120.2.934-944.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wyman L., Goering R. V., Novick R. P. Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics. 1974 Apr;76(4):681–702. doi: 10.1093/genetics/76.4.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wyman L., Novick R. P. Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid. Mol Gen Genet. 1974;135(2):149–161. doi: 10.1007/BF00264782. [DOI] [PubMed] [Google Scholar]