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. 1977 Jan;129(1):246–253. doi: 10.1128/jb.129.1.246-253.1977

Biosynthesis of bacterial glycogen: genetic and allosteric regulation of glycogen biosynthesis in Salmonella typhimurium LT-2.

K E Steiner, J Preiss
PMCID: PMC234921  PMID: 401493

Abstract

Structural gene mutants of the glycogen biosynthetic enzymes adenosine diphosphate glucose pyrophosphorylase (glgC) and glycogen synthase (glgA) were isolated and partially characterized. The cotransduction frequencies of these genes with the aspartic semialdehyde dehydrogenase (asd) and glycerol-3-phosphate dehydrogenase (glpD) genes suggested the unambiguous gene order of glpD glgA glgC asd. The results of the three-factor cross glpD- glgA- glgC+ X glpD+ glgA+ glgC- were consistent with the proposed order. A simultaneous and approximately equivalent derepression of the glgC, glgA, and glgB (branching enzyme) gene products was observed in the late logarithmic-early stationary phase of growth on enriched media. These results are consistent with the coordinately regulated synthesis of the three glycogen biosynthetic enzymes in Salmonella typhimurium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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