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. 1977 Jan;129(1):472–481. doi: 10.1128/jb.129.1.472-481.1977

Deoxyribonucleic acid sequence organization of a yeast plasmid.

D M Livingston, H L Klein
PMCID: PMC234946  PMID: 318650

Abstract

Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demonstrated the existence of two forms of 2-mum DNA differing in the orientation of the interstitial segments bounded by the inverted repeated sequences. The two forms of 2-mum DNA could result from an intramolecular reciprocal recombination between inverted repeat elements. A map containing the restriction endonuclease sites and the units of the inverted repeat has been constructed.

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Selected References

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