Abstract
This study demonstrates, for the first time, the autolytic enzymes associated with mycobacterial cell walls. Based on the release of radioactivity and ninhydrin-reactive material from isolated cell walls, it was shown that maximum activity occurs during the late log phase of growth and at a buffer pH of about 8.0. Chemical analyses of autolytic digests of isolated cell walls indicated that at least three autolysins are active under the conditions used. These are N-glycolylmuramic acid-L-alanine amidase, an aminopeptidase that releases L-alanine, and an endopeptidase that solubilizes and L-alanyl-D-glutamic acid dippetide. No other endopeptidase, carboxypeptidase, or glycosidase activity was detected.
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- Azuma I., Thomas D. W., Adam A., Ghuysen J. M., Bonaly R., Petit J. F., Lederer E. Occurrence of N-glycolylmuramic acid in bacterial cell walls. A preliminary survey. Biochim Biophys Acta. 1970 Jun;208(3):444–451. doi: 10.1016/0304-4165(70)90217-5. [DOI] [PubMed] [Google Scholar]
- Best G. K., Mattingly S. J. Chemical analysis of cell walls and autolytic digests of Bacillus psychrophilus. J Bacteriol. 1973 Jul;115(1):221–227. doi: 10.1128/jb.115.1.221-227.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chan L., Glaser L. Purification of N-acetylmuramic acid-L-alanine amidase from Bacillus megaterium. J Biol Chem. 1972 Sep 10;247(17):5391–5397. [PubMed] [Google Scholar]
- Ghuysen J. M. Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism. Bacteriol Rev. 1968 Dec;32(4 Pt 2):425–464. [PMC free article] [PubMed] [Google Scholar]
- Higgins M. L., Shockman G. D. Procaryotic cell division with respect to wall and membranes. CRC Crit Rev Microbiol. 1971 May;1(1):29–72. doi: 10.3109/10408417109104477. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lederer E. The mycobacterial cell wall. Pure Appl Chem. 1971;25(1):135–165. doi: 10.1351/pac197125010135. [DOI] [PubMed] [Google Scholar]
- Lindsay B., Glaser L. Characterization of the N-acetylmuramic acid L-alanine amidase from Bacillus subtilis. J Bacteriol. 1976 Aug;127(2):803–811. doi: 10.1128/jb.127.2.803-811.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan W. T., Elson L. A. A colorimetric method for the determination of N-acetylglucosamine and N-acetylchrondrosamine. Biochem J. 1934;28(3):988–995. doi: 10.1042/bj0280988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PARK J. T., JOHNSON M. J. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed] [Google Scholar]
- Petit J. F., Adam A., Wietzerbin-Falszpan J., Lederer E., Ghuysen J. M. Chemical structure of the cell wall of Mycobacterium smegmatis. I. Isolation and partial characterization of the peptidoglycan. Biochem Biophys Res Commun. 1969 May 22;35(4):478–485. doi: 10.1016/0006-291x(69)90371-4. [DOI] [PubMed] [Google Scholar]
- Pooley H. M., Porres-Juan J. M., Shockman G. D. Dissociation of an autolytic enzyme-cell wall complex by treatment with unusually high concentrations of salt. Biochem Biophys Res Commun. 1970 Mar 27;38(6):1134–1140. doi: 10.1016/0006-291x(70)90357-8. [DOI] [PubMed] [Google Scholar]
- ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. doi: 10.1016/0003-9861(57)90241-2. [DOI] [PubMed] [Google Scholar]