Abstract
Strains of Escherichia coli B/r containing a deletion of the regulatory gene araC are Ara-. Slow-growing revertants of these strains were isolated and designated aralc because they contain a second mutation in a controlling site, aral, that allows for a low level of constitutive expression of the araBAD operon (Englesbert et al., 1969). We mutagenized aralc delta C strains and selected mutants that grow faster in mineral L-arabinose medium. The new mutations, called araXc, map very close to the original aralc mutations and are in the controlling site region between araB and araC. The aralcXc delta C strains have a higher constitutive level of expression of the araBAD operon than the aralc delta C parents. The araXc mutations are cis acting and decrease the araBAD operon's sensitivity to catabolite repression. The araBAD operon is expressed equally well in ara delta C and ara C cya crp backgrounds. The repressor form of ara C protein is able to repress the constitutive synthesis due to the ara Xc allele.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bass R., Heffernan L., Sweadner K., Englesberg E. The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r. Arch Microbiol. 1976 Oct 11;110(1):135–143. doi: 10.1007/BF00416978. [DOI] [PubMed] [Google Scholar]
- CRIBBS R., ENGLESBERG E. L-ARABINOSE NEGATIVE MUTANTS OF THE L-RIBULOKINASE STRUCTURAL GENE AFFECTING THE LEVELS OF L-ARABINOSE ISOMERASE IN ESCHERICHIA COLI. Genetics. 1964 Jan;49:95–108. doi: 10.1093/genetics/49.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ENGLESBERG E. Enzymatic characterization of 17 L-arabinose negative mutants of Escherichia coli. J Bacteriol. 1961 Jun;81:996–1006. doi: 10.1128/jb.81.6.996-1006.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Englesberg E., Irr J., Power J., Lee N. Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol. 1965 Oct;90(4):946–957. doi: 10.1128/jb.90.4.946-957.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Englesberg E., Sheppard D., Squires C., Meronk F., Jr An analysis of "revertants" of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B-r: isolation of initiator constitutive mutants (Ic). J Mol Biol. 1969 Jul 28;43(2):281–298. doi: 10.1016/0022-2836(69)90268-x. [DOI] [PubMed] [Google Scholar]
- Englesberg E., Squires C., Meronk F., Jr The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1100–1107. doi: 10.1073/pnas.62.4.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Englesberg E., Wilcox G. Regulation: positive control. Annu Rev Genet. 1974;8:219–242. doi: 10.1146/annurev.ge.08.120174.001251. [DOI] [PubMed] [Google Scholar]
- GROSS J., ENGLESBERG E. Determination of the order of mutational sites governing L-arabinose utilization in Escherichia coli B/r bv transduction with phage Plbt. Virology. 1959 Nov;9:314–331. doi: 10.1016/0042-6822(59)90125-4. [DOI] [PubMed] [Google Scholar]
- Gielow L., Largen M., Englesberg E. Initiator constitutive mutants of the L-arabinose operon (OIBAD) of Escherichia coli B/r. Genetics. 1971 Nov;69(3):289–302. doi: 10.1093/genetics/69.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heffernan L., Bass R., Englesberg E. Mutations affecting catabolite repression of the L-arabinose regulon in Escherichia coli B/r. J Bacteriol. 1976 Jun;126(3):1119–1131. doi: 10.1128/jb.126.3.1119-1131.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katz L., Englesberg E. Hyperinducibility as a result of mutation in structural genes and self-catabolite repression in the ara operon. J Bacteriol. 1971 Jul;107(1):34–52. doi: 10.1128/jb.107.1.34-52.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kessler D. P., Englesberg E. Arabinose-leucine deletion mutants of Escherichia coli B-r. J Bacteriol. 1969 Jun;98(3):1159–1169. doi: 10.1128/jb.98.3.1159-1169.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Sheppard D. E., Englesberg E. Further evidence for positive control of the L-arabinose system by gene araC. J Mol Biol. 1967 May 14;25(3):443–454. doi: 10.1016/0022-2836(67)90197-0. [DOI] [PubMed] [Google Scholar]