Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Feb;129(2):967–972. doi: 10.1128/jb.129.2.967-972.1977

Isolation of an Escherichia coli mutant deficient in thioredoxin reductase.

J Fuchs
PMCID: PMC235035  PMID: 14115

Abstract

A mutant of Escherichia coli defective in thioredoxin reductase has been isolated and partially characterized. This mutant has no detectable thioredoxin reductase activity in vitro and yet it exhibits no in vivo defect in reduction of ribonucleotides. Evidence is presented that indicates that, in cells permeabilized via ether treatment, ribonucleoside diphosphate reduction can utilize glutathione as an alternate reducing system.

Full text

PDF
967

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apontoweil P., Berends W. Isolation and initial characterization of glutathione-deficient mutants of Escherichia coli K 12. Biochim Biophys Acta. 1975 Jul 14;399(1):10–22. doi: 10.1016/0304-4165(75)90206-8. [DOI] [PubMed] [Google Scholar]
  2. Brown N. C., Canellakis Z. N., Lundin B., Reichard P., Thelander L. Ribonucleoside diphosphate reductase. Purification of the two subunits, proteins B1 and B2. Eur J Biochem. 1969 Jul;9(4):561–573. doi: 10.1111/j.1432-1033.1969.tb00646.x. [DOI] [PubMed] [Google Scholar]
  3. Brown N. C., Larsson A., Reichard P. On the subunit structure of ribonucleoside diphosphate reductase. J Biol Chem. 1967 Sep 25;242(18):4272–4273. [PubMed] [Google Scholar]
  4. Fuchs J. A., Warner H. R. Isolation of an Escherichia coli mutant deficient in glutathione synthesis. J Bacteriol. 1975 Oct;124(1):140–148. doi: 10.1128/jb.124.1.140-148.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gonzalez Porqué P., Baldesten A., Reichard P. Purification of a thioredoxin system from yeast. J Biol Chem. 1970 May 10;245(9):2363–2370. [PubMed] [Google Scholar]
  6. Gonzalez Porqué P., Baldesten A., Reichard P. The involvement of the thioredoxin system in the reduction of methionine sulfoxide and sulfate. J Biol Chem. 1970 May 10;245(9):2371–2374. [PubMed] [Google Scholar]
  7. Greene R. C. Methionine limitation in Escherichia coli K-12 by growth on the sulfoxides of D-methionine. J Bacteriol. 1973 Oct;116(1):230–234. doi: 10.1128/jb.116.1.230-234.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Herrmann E. C., Moore E. C. Purification of thioredoxin from rat Novikoff ascites hepatoma. J Biol Chem. 1973 Feb 25;248(4):1219–1223. [PubMed] [Google Scholar]
  9. Holmgren A. Hydrogen donor system for Escherichia coli ribonucleoside-diphosphate reductase dependent upon glutathione. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2275–2279. doi: 10.1073/pnas.73.7.2275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karlström O. Mutants of Escherichia coli defective in ribonucleoside and deoxyribonucleoside catabolism. J Bacteriol. 1968 Mar;95(3):1069–1077. doi: 10.1128/jb.95.3.1069-1077.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LAURENT T. C., MOORE E. C., REICHARD P. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES. IV. ISOLATION AND CHARACTERIZATION OF THIOREDOXIN, THE HYDROGEN DONOR FROM ESCHERICHIA COLI B. J Biol Chem. 1964 Oct;239:3436–3444. [PubMed] [Google Scholar]
  12. Larson G., Larsson A. Purification and properties of rat-liver thioredoxin. Eur J Biochem. 1972 Mar 15;26(1):119–124. doi: 10.1111/j.1432-1033.1972.tb01747.x. [DOI] [PubMed] [Google Scholar]
  13. Larsson A. Thioredoxin reductase from rat liver. Eur J Biochem. 1973 Jun;35(2):346–349. doi: 10.1111/j.1432-1033.1973.tb02845.x. [DOI] [PubMed] [Google Scholar]
  14. MOORE E. C., REICHARD P., THELANDER L. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES.V. PURIFICATION AND PROPERTIES OF THIOREDOXIN REDUCTASE FROM ESCHERICHIA COLI B. J Biol Chem. 1964 Oct;239:3445–3452. [PubMed] [Google Scholar]
  15. Mark D. F., Richardson C. C. Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1976 Mar;73(3):780–784. doi: 10.1073/pnas.73.3.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Munavalli S., Parker D. V., Hamilton F. D. Identification of NADPH-thioredoxin reductase system in Euglena gracillis. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4233–4237. doi: 10.1073/pnas.72.11.4233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Orr M. D., Vitols E. Thioredoxin from Lactobacillus leichmannii and its role as hydrogen donor for ribonucleoside triphosphate reductase. Biochem Biophys Res Commun. 1966 Oct 5;25(1):109–115. doi: 10.1016/0006-291x(66)90647-4. [DOI] [PubMed] [Google Scholar]
  18. REICHARD P. Enzymatic synthesis of deoxyribonucleotides. I. Formation of deoxycytidine diphosphate from cytidine diphosphate with enzymes from Escherichia coli. J Biol Chem. 1962 Nov;237:3513–3519. [PubMed] [Google Scholar]
  19. SOURKES T. L., TRANO Y. Reduction of methionine sulfoxides by Escherichia coli. Arch Biochem Biophys. 1953 Feb;42(2):321–326. doi: 10.1016/0003-9861(53)90361-0. [DOI] [PubMed] [Google Scholar]
  20. Thelander L. Reaction mechanism of ribonucleoside diphosphate reductase from Escherichia coli. Oxidation-reduction-active disulfides in the B1 subunit. J Biol Chem. 1974 Aug 10;249(15):4858–4862. [PubMed] [Google Scholar]
  21. Tsang M. L., Schiff J. A. Sulfate-reducing pathway in Escherichia coli involving bound intermediates. J Bacteriol. 1976 Mar;125(3):923–933. doi: 10.1128/jb.125.3.923-933.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Warner H. R. Properties of ribonucleoside diphosphate reductase in nucleotide-permeable cells. J Bacteriol. 1973 Jul;115(1):18–22. doi: 10.1128/jb.115.1.18-22.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES