Abstract
Five mutants of Bacillus subtilis 168 defective in an intracellular esterase activity were identified. By polyacrylamide gel electrophoresis, four of the mutants were shown to lack esterase B activity, and the fifth lacked esterase A activity. All of the back-crossed esterase mutants were able to sporulate at wild-type frequency and produce exoprotease(s) and antibiotic(s). No difference in motility could be attributed to the esterase mutation. PBS1 transduction analysis showed all the esterase B mutations to be linked to the hisA marker.
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- Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baptist J. N., Shaw C. R., Mandel M. Comparative zone electrophoresis of enzymes of Pseudomonas solanacearum and Pseudomonas cepacia. J Bacteriol. 1971 Nov;108(2):799–803. doi: 10.1128/jb.108.2.799-803.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bott K. F. Acrylamide gel electrophoresis of intracellular proteins during early stages of sporulation in Bacillus subtilis. J Bacteriol. 1971 Nov;108(2):720–732. doi: 10.1128/jb.108.2.720-732.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greenleaf A. L., Losick R. Appearance of a ribonucleic acid polymerase-binding protein in asporogenous mutants of Bacillus subtilis. J Bacteriol. 1973 Oct;116(1):290–294. doi: 10.1128/jb.116.1.290-294.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higerd T. B., Spizizen J. Isolation of two acetyl esterases from extracts of Bacillus subtilis. J Bacteriol. 1973 Jun;114(3):1184–1192. doi: 10.1128/jb.114.3.1184-1192.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoch J. A. Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis. J Bacteriol. 1971 Mar;105(3):896–901. doi: 10.1128/jb.105.3.896-901.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ito J., Spizizen J. Increased rate of asporogenous mutations following treatment of Bacillus subtilis spores with ethyl methanesulfonate. Mutat Res. 1971 Sep;13(1):93–96. doi: 10.1016/0027-5107(71)90130-8. [DOI] [PubMed] [Google Scholar]
- Koch A. L., Putnam S. L. Sensitive biuret method for determination of protein in an impure system such as whole bacteria. Anal Biochem. 1971 Nov;44(1):239–245. doi: 10.1016/0003-2697(71)90366-6. [DOI] [PubMed] [Google Scholar]
- Lund B. M. A comparison by the use of gel electrophoresis of soluble protein components and esterase enzymes of some group D Streptococci. J Gen Microbiol. 1965 Sep;40(3):413–419. doi: 10.1099/00221287-40-3-413. [DOI] [PubMed] [Google Scholar]
- Mandelstam J., Waites W. M. Sporulation in Bacillus subtilis. The role of exoprotease. Biochem J. 1968 Oct;109(5):793–801. doi: 10.1042/bj1090793. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michel J. F., Millet J. Physiological studies on early-blocked sporulation mutants of Bacillus subtilis. J Appl Bacteriol. 1970 Mar;33(1):220–227. doi: 10.1111/j.1365-2672.1970.tb05246.x. [DOI] [PubMed] [Google Scholar]
- Millet J. Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. J Appl Bacteriol. 1970 Mar;33(1):207–219. doi: 10.1111/j.1365-2672.1970.tb05245.x. [DOI] [PubMed] [Google Scholar]
- Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]