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. 1977 Feb;129(2):1045–1050. doi: 10.1128/jb.129.2.1045-1050.1977

Morphological changes associated with novobiocin resistance in Bacillus licheniformis.

R L Robson, J Baddiley
PMCID: PMC235044  PMID: 838675

Abstract

Spontaneously occurring novobiocin-resistant (Nov) mutants of Bacillus licheniformis ATCC 9945, resistant to low levels of novobiocin (15 mug/ml), were isolated with a frequency of 3 in 106 organisms. Such isolates grew well, but nearly all exhibited consistent plleiotropic alterations in colonial and cell morphologies. One mutant, nov-12, grew as chains of unseparated but clearly distinct daughter cells in the absence of novobiocin in liquid culture. When novobiocin was present, nov-12 grew as very long "filaments" which were, however, septate. Septa formed in the presence of the antibiotic were normal, except that no annular clevage of the septal wall was observed. Septa were also irregularly positioned along the filament. These observations were compared with previous findings on the effects of novobiocin and novobiocin resistance described for other organisms. It was concluded that the primary action of novobiocin might differ in gram-positive and gram-negative organisms. However, when the low-level novobiocin sensitivity, normally associated with gram-positive organisms, was genetically abolished in Nov strains of B. licheniformis they became susceptible to an action of novobiocin more analogous to that found for gram-negative organisms. The morphological alterations associated with the Nov phenotype in this organism, together with observations in other organisms, indicate that novobiocin resistance might be generally useful in the search for mutants of gram-positive organisms with altered cell walls.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAMS A., McNAMARA P., JOHNSON F. B. Adenosine triphosphatase in isolated bacterial cell membranes. J Biol Chem. 1960 Dec;235:3649–3662. [PubMed] [Google Scholar]
  2. Allwood M. C., Russell A. D. Thermally induced changes in the physical properties of Staphylococcus aureus. J Appl Bacteriol. 1969 Mar;32(1):68–78. doi: 10.1111/j.1365-2672.1969.tb02190.x. [DOI] [PubMed] [Google Scholar]
  3. Anderson R. G., Douglas L. J., Hussey H., Baddiley J. The control of synthesis of bacterial cell walls. Interaction in the synthesis of nucleotide precursors. Biochem J. 1973 Dec;136(4):871–876. doi: 10.1042/bj1360871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BROCK T. D., BROCK M. L. Effect of novobiocin on permeability of Escherichia coli. Arch Biochem Biophys. 1959 Nov;85:176–185. doi: 10.1016/0003-9861(59)90461-8. [DOI] [PubMed] [Google Scholar]
  5. BROCK T. D. Studies on the mode of action of novobiocin. J Bacteriol. 1956 Sep;72(3):320–323. doi: 10.1128/jb.72.3.320-323.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burger M. M., Glaser L. The synthesis of teichoic acids. V. Polyglucosylglycerol phosphate and polygalactosylglycerol phosphate. J Biol Chem. 1966 Jan 25;241(2):494–506. [PubMed] [Google Scholar]
  7. Chatterjee A. N., Mirelman D., Singer H. J., Park J. T. Properties of a novel pleiotropic bacteriophage-resistant mutant of Staphylococcus aureus H. J Bacteriol. 1969 Nov;100(2):846–853. doi: 10.1128/jb.100.2.846-853.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. ELLIOTT H. J., HALL W. H. Studies on synergism with twelve antibiotics against thirty hospital strains of Staphylococcus aureus. J Lab Clin Med. 1957 Aug;50(2):242–249. [PubMed] [Google Scholar]
  9. Fan D. P., Beckman M. M. Mutant of Bacillus subtilis demonstrating the requirement of lysis for growth. J Bacteriol. 1971 Feb;105(2):629–636. doi: 10.1128/jb.105.2.629-636.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Forsberg C. W., Rogers H. J. Characterization of Bacillus licheniformis 6346 mutants which have altered lytic enzyme activities. J Bacteriol. 1974 May;118(2):358–368. doi: 10.1128/jb.118.2.358-368.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Forsberg C., Rogers H. J. Autolytic enzymes in growth of bacteria. Nature. 1971 Jan 22;229(5282):272–273. doi: 10.1038/229272a0. [DOI] [PubMed] [Google Scholar]
  12. GLASER L. THE SYNTHESIS OF TEICHOIC ACIDS. II. POLYRIBITOL PHOSPHATE. J Biol Chem. 1964 Oct;239:3178–3186. [PubMed] [Google Scholar]
  13. Hancock I. C., Baddiley J. Biosynthesis of the wall teichoic acid in Bacillus licheniformis. Biochem J. 1972 Mar;127(1):27–37. doi: 10.1042/bj1270027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hughes R. C. The cell wall of Bacillus licheniformis N.C.T.C. 6346: Biosynthesis of the teichuronic acid. Biochem J. 1966 Dec;101(3):692–697. doi: 10.1042/bj1010692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ishimoto N., Strominger J. L. Polyribitol phosphate synthetase of Staphylococcus aureus. J Biol Chem. 1966 Feb 10;241(3):639–650. [PubMed] [Google Scholar]
  16. KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. KORMAN R. Z. COAGULASE-NEGATIVE MUTANTS OF STAPHYLOCOCCUS AUREUS: GENETIC STUDIES. J Bacteriol. 1963 Sep;86:363–369. doi: 10.1128/jb.86.3.363-369.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Korman R. Z. Bacteriophage-typable revertants from pleiotropic staphylococcal mutants. J Bacteriol. 1975 Nov;124(2):731–735. doi: 10.1128/jb.124.2.731-735.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Korman R. Z. Cell wall composition of novobiocin-resistant pleiotropic mutant staphylococci. J Bacteriol. 1975 Nov;124(2):724–730. doi: 10.1128/jb.124.2.724-730.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morris A., Russell A. D. Studies on the mode of action of novobiocin. Biochem Pharmacol. 1968 Sep;17(9):1923–1929. doi: 10.1016/0006-2952(68)90108-1. [DOI] [PubMed] [Google Scholar]
  21. Pooley H. M., Shockman G. D., Higgins M. L., Porres-Juan J. Some properties of two autolytic-defective mutants of Streptococcus faecalis ATCC 9790. J Bacteriol. 1972 Jan;109(1):423–431. doi: 10.1128/jb.109.1.423-431.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Robson R. L., Baddiley J. Role of teichuronic acid in Bacillus licheniformis: defective autolysis due to deficiency of teichuronic acid in a novobiocin-resistant mutant. J Bacteriol. 1977 Feb;129(2):1051–1058. doi: 10.1128/jb.129.2.1051-1058.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. STROMINGER J. L., THRENN R. H. The optical configuration of the alanine residues in a uridine nucleotide and in the cell wall of Staphylococcus aureus. Biochim Biophys Acta. 1959 May;33(1):280–281. doi: 10.1016/0006-3002(59)90538-4. [DOI] [PubMed] [Google Scholar]
  25. Smith D. H., Davis B. D. Mode of action of novobiocin in Escherichia coli. J Bacteriol. 1967 Jan;93(1):71–79. doi: 10.1128/jb.93.1.71-79.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Soper J. W., Winter C. G. Role of cell wall antolysin in chain formation by a mutant strain of Streptococcus faecalis. Biochim Biophys Acta. 1973 Feb 28;297(2):333–342. doi: 10.1016/0304-4165(73)90080-9. [DOI] [PubMed] [Google Scholar]
  27. Sterlini J. M., Mandelstam J. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WISHNOW R. M., STROMINGER J. L., BIRGE C. H., THRENN R. H. BIOCHEMICAL EFFECTS OF NOVOBIOCIN ON STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Apr;89:1117–1123. doi: 10.1128/jb.89.4.1117-1123.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yamada M., Hirose A., Matsuhashi M. Association of lack of cell wall teichuronic acid with formation of cell packets of Micrococcus lysodeikticus (luteus) mutants. J Bacteriol. 1975 Aug;123(2):678–686. doi: 10.1128/jb.123.2.678-686.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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