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. 1977 Feb;129(2):1066–1071. doi: 10.1128/jb.129.2.1066-1071.1977

Volume-related mitochondrial deoxyribonucleic acid synthesis in zygotes and vegetative cells of Saccharomyces cerevisiae.

E Lee, B F Johnson
PMCID: PMC235047  PMID: 320176

Abstract

The synthesis of mitochondrial deoxyribonucleic acid (DNA) in Saccharomyces cerevisiae cells has been examined during conjugation, in preconjugal conditions, and in control cultures that were not exposed to obverse diffusible sex factors. The ratios of mitochondrial to nuclear DNA varied from about 0.1 in control cells, to about 0.3 in alpha cells exposed for 180 min to cell-free culture medium from a cells, and to about 0.4 in conjugating cells 150 min after mixing. The enhanced levels of mitochondrial DNA during preconjugal and conjugal conditions seem correlated with enhanced cell volumes. Likewise, amounts of mitochondrial DNA in vegetative cells were found to be correlated with cytoplasmic volumes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res. 1973 Jan;76(1):99–110. doi: 10.1016/0014-4827(73)90424-2. [DOI] [PubMed] [Google Scholar]
  2. Dawes I. W., Carter B. L. Nitrosoguanidine mutagenesis during nuclear and mitochondrial gene replication. Nature. 1974 Aug 30;250(5469):709–712. doi: 10.1038/250709a0. [DOI] [PubMed] [Google Scholar]
  3. Duntze W., MacKay V., Manney T. R. Saccharomyces cerevisiae: a diffusible sex factor. Science. 1970 Jun 19;168(3938):1472–1473. doi: 10.1126/science.168.3938.1472. [DOI] [PubMed] [Google Scholar]
  4. Ephrussi B., Jakob H., Grandchamp S. Etudes Sur La SuppressivitE Des Mutants a Deficience Respiratoire De La Levure. II. Etapes De La Mutation Grande En Petite Provoquee Par Le Facteur Suppressif. Genetics. 1966 Jul;54(1):1–29. doi: 10.1093/genetics/54.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldthwaite C. D., Cryer D. R., Marmur J. Effect of carbon source on the replication and transmission of yeast mitochondrial genomes. Mol Gen Genet. 1974;133(2):87–104. doi: 10.1007/BF00264830. [DOI] [PubMed] [Google Scholar]
  6. Grossman L. I., Goldring E. S., Marmur J. Preferential synthesis of yeast mitochondrial DNA in the absence of protein synthesis. J Mol Biol. 1969 Dec 28;46(3):367–376. doi: 10.1016/0022-2836(69)90182-x. [DOI] [PubMed] [Google Scholar]
  7. Lee E. H., Lusena C. V., Johnson B. F. A new method of obtaining zygotes in Saccharomyces cerevisiae. Can J Microbiol. 1975 Jun;21(6):802–806. doi: 10.1139/m75-118. [DOI] [PubMed] [Google Scholar]
  8. Michaelis G., Petrochilo E., Slonimski P. P. Mitochondrial genetics. 3. Recombined molecules of mitochondrial DNA obtained from crosses between cytoplasmic petite mutants of Saccharomyces cerevisiae: physical and genetic characterization. Mol Gen Genet. 1973;123(1):51–65. doi: 10.1007/BF00282988. [DOI] [PubMed] [Google Scholar]
  9. Michels C. A., Blamire J., Goldfinger B., Marmur J. A genetic and biochemical analysis of petite mutations in yeast. J Mol Biol. 1974 Dec 15;90(3):431–449. doi: 10.1016/0022-2836(74)90226-5. [DOI] [PubMed] [Google Scholar]
  10. Newlon C. S., Fangman W. L. Mitochondrial DNA synthesis in cell cycle mutants of Saccharomyces cerevisiae. Cell. 1975 Aug;5(4):423–428. doi: 10.1016/0092-8674(75)90061-6. [DOI] [PubMed] [Google Scholar]
  11. Petes T. D., Fangman W. L. Preferential synthesis of yeast mitochondrial DNA in alpha factor-arrested cells. Biochem Biophys Res Commun. 1973 Dec 10;55(3):603–609. doi: 10.1016/0006-291x(73)91186-8. [DOI] [PubMed] [Google Scholar]
  12. Sena E. P., Welch J. W., Halvorson H. O., Fogel S. Nuclear and mitochondrial deoxyribonucleic acid replication during mitosis in Saccharomyces cerevisiae. J Bacteriol. 1975 Aug;123(2):497–504. doi: 10.1128/jb.123.2.497-504.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shannon C., Rao A., Douglass S., Criddle R. S. Recombination in yeast mitochondrial DNA. J Supramol Struct. 1972;1(2):145–152. doi: 10.1002/jss.400010207. [DOI] [PubMed] [Google Scholar]
  14. Wilkinson L. E., Pringle J. R. Transient G1 arrest of S. cerevisiae cells of mating type alpha by a factor produced by cells of mating type a. Exp Cell Res. 1974 Nov;89(1):175–187. doi: 10.1016/0014-4827(74)90200-6. [DOI] [PubMed] [Google Scholar]
  15. Williamson D. H., Fennell D. J. Apparent dispersive replication of yeast mitochondrial DNA as revealed by density labelling experiments. Mol Gen Genet. 1974;131(3):193–207. doi: 10.1007/BF00267959. [DOI] [PubMed] [Google Scholar]
  16. Williamson D. H., Moustacchi E., Fennell D. A procedure for rapidly extracting and estimating the nuclear and cytoplasmic DNA components of yeast cells. Biochim Biophys Acta. 1971 May 13;238(2):369–374. doi: 10.1016/0005-2787(71)90106-7. [DOI] [PubMed] [Google Scholar]
  17. Williamson D. H. The effect of environmental and genetic factors on the replication of mitochondrial DNA in yeast. Symp Soc Exp Biol. 1970;24:247–276. [PubMed] [Google Scholar]
  18. Zeman L., Lusena C. V. DNA synthesis in isolated yeast mitochondria. Can J Biochem. 1974 Nov;52(11):941–949. doi: 10.1139/o74-132. [DOI] [PubMed] [Google Scholar]
  19. Zuk J., Zaborowska D., Litwińska J., Chlebowicz E., Biliński T. Macromolecular synthesis during conjugation in yeast. Acta Microbiol Pol A. 1975;7(2):67–75. [PubMed] [Google Scholar]

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