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. 1977 Mar;129(3):1222–1226. doi: 10.1128/jb.129.3.1222-1226.1977

Two unlinked genes for the pyruvate dehydrogenase complex in Aspergillus nidulans.

M A Payton, W McCullough, C F Roberts, J R Guest
PMCID: PMC235084  PMID: 321417

Abstract

The activity of the overall pyruvate dehydrogenase complex was found to be similar in extracts of Aspergillus nidulans after growth on either sucrose or acetate. Eight mutants lacking the activity of this complex were found among some 200 glycolytic mutants selected for their inability to grow on sucrose. The absence of pyruvate dehydrogenase complex activity was also confirmed for a mutant, g6 (pdhA1), isolated previously. Studies with the mutants supported the existence of two unlinked genes, pdhA and pdhB, controlling the function of the complex. In vivo and in vitro complementation between mutations at the two loci were shown by the ability of forced heterokaryons to grow on sucrose and by the restoration of overall pyruvate dehydrogenase complex activity in mixed cell-free extracts. The mutations were recessive to their wild-type alleles, and the pdhA and pdhB loci were assigned to linkage groups I and V, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderson T., Hartley M. J. Specificity for spontaneous and induced forward mutation at several gene loci in Aspergillus nidulans. Mutat Res. 1969 Sep-Oct;8(2):255–264. doi: 10.1016/0027-5107(69)90005-0. [DOI] [PubMed] [Google Scholar]
  2. Amarasingham C. R., Davis B. D. Regulation of alpha-ketoglutarate dehydrogenase formation in Escherichia coli. J Biol Chem. 1965 Sep;240(9):3664–3668. [PubMed] [Google Scholar]
  3. Armitt S., McCullough W., Roberts C. F. Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans. J Gen Microbiol. 1976 Feb;92(2):263–282. doi: 10.1099/00221287-92-2-263. [DOI] [PubMed] [Google Scholar]
  4. Bresters T. W., Krul J., Scheepens P. C., Veeger C. Phosphotransacetylase associated with the pyruvate dehydrogenase complex from the nitrogen fixing Azotobacter vinelandii. FEBS Lett. 1972 May 15;22(3):305–309. doi: 10.1016/0014-5793(72)80257-6. [DOI] [PubMed] [Google Scholar]
  5. Guest J. R. Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: Chromosomal location of the lipoamide dehydrogenase gene. J Gen Microbiol. 1974 Feb;80(2):523–532. doi: 10.1099/00221287-80-2-523. [DOI] [PubMed] [Google Scholar]
  6. HAGER L. P., KORNBERG H. L. On the mechanism of alpha-oxoglutarate oxidation in Escherichia coli. Biochem J. 1961 Jan;78:194–198. doi: 10.1042/bj0780194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HENNING U., HERZ C. EIN STRUKTURGEN-KOMPLEX FUER DEN PYRUVAT-DEHYDROGENASE-KOMPLEX VON ESCHERICHIA COLI K 12. Z Vererbungsl. 1964 Nov 11;95:260–275. [PubMed] [Google Scholar]
  8. Harding R. W., Caroline D. F., Wagner R. P. The pyruvate dehydrogenase complex from the mitochondrial fraction of Neurospora crassa. Arch Biochem Biophys. 1970 Jun;138(2):653–661. doi: 10.1016/0003-9861(70)90393-0. [DOI] [PubMed] [Google Scholar]
  9. Kappas A., Georgopoulos S. G., Hastie A. C. On the genetic activity of benzimidazole and thiophanate fungicides on diploid Aspergillus nidulans. Mutat Res. 1974 Feb;26(1):17–27. doi: 10.1016/s0027-5107(74)80066-7. [DOI] [PubMed] [Google Scholar]
  10. Langley D., Guest J. R. Biochemical and genetic characterics of deletion and other mutant strains of Salmonella typhimurium LT2 lacking alpha-keto acid dehydrogenase complex activities,. J Gen Microbiol. 1974 Jun;82(2):319–335. doi: 10.1099/00221287-82-2-319. [DOI] [PubMed] [Google Scholar]
  11. Linn T. C., Pettit F. H., Hucho F., Reed L. J. Alpha-keto acid dehydrogenase complexes. XI. Comparative studies of regulatory properties of the pyruvate dehydrogenase complexes from kidney, heart, and liver mitochondria. Proc Natl Acad Sci U S A. 1969 Sep;64(1):227–234. doi: 10.1073/pnas.64.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McCully K. S., Forbes E. The use of p-fluorophenylalanine with 'master strains' of Aspergillus nidulans for assigning genes to linkage groups. Genet Res. 1965 Nov;6(3):352–359. doi: 10.1017/s0016672300004249. [DOI] [PubMed] [Google Scholar]
  13. PONTECORVO G., ROPER J. A., HEMMONS L. M., MACDONALD K. D., BUFTON A. W. J. The genetics of Aspergillus nidulans. Adv Genet. 1953;5:141–238. doi: 10.1016/s0065-2660(08)60408-3. [DOI] [PubMed] [Google Scholar]
  14. Payton M., Roberts C. F. Mutants of Aspergillus nidulans lacking pyruvate kinase. FEBS Lett. 1976 Jul 1;66(1):73–76. doi: 10.1016/0014-5793(76)80588-1. [DOI] [PubMed] [Google Scholar]
  15. REED L. J., LEACH F. R., KOIKE M. Studies on a lipoic acid-activating system. J Biol Chem. 1958 May;232(1):123–142. [PubMed] [Google Scholar]
  16. Reed L. J., Pettit F. H., Eley M. H., Hamilton L., Collins J. H., Oliver R. M. Reconstitution of the Escherichia coli pyruvate dehydrogenase complex. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3068–3072. doi: 10.1073/pnas.72.8.3068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Romano A. H., Kornberg H. L. Regulation of sugar utilization by Aspergillus nidulans. Biochim Biophys Acta. 1968 Jun 24;158(3):491–493. doi: 10.1016/0304-4165(68)90312-7. [DOI] [PubMed] [Google Scholar]
  18. Schwartz E. R., Reed L. J. Regulation of the activity of the pyruvate dehydrogenase complex of Escherichia coli. Biochemistry. 1970 Mar 17;9(6):1434–1439. doi: 10.1021/bi00808a019. [DOI] [PubMed] [Google Scholar]
  19. Skinner V. M., Armitt S. Mutants of Aspergillus nidulans lacking pyruvate carboxylase. FEBS Lett. 1972 Jan 15;20(1):16–18. doi: 10.1016/0014-5793(72)80005-x. [DOI] [PubMed] [Google Scholar]
  20. Wieland O. H., Hartmann U., Siess E. A. Neurospora crassa pyruvate dehydrogenase: interconversion by phosphorylation and dephosphorylation. FEBS Lett. 1972 Nov 1;27(2):240–244. doi: 10.1016/0014-5793(72)80630-6. [DOI] [PubMed] [Google Scholar]

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