Abstract
Optimal conditions for the 24(28)methylene reductase were obtained. The enzyme assay provided for unusually high activity; the Km was determined to be 10.8 mum. The enzyme activity was increased in cells grown with ethanol as the substrate.
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Selected References
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- Anding C., Brandt R. D., Ourisson G. Sterol biosynthesis in Euglena gracilis Z. Sterol precursors in light-grown and dark-grown Euglena gracilis Z. Eur J Biochem. 1971 Dec;24(2):259–263. doi: 10.1111/j.1432-1033.1971.tb19679.x. [DOI] [PubMed] [Google Scholar]
- Bailey R. B., Hays P. R., Parks L. W. Homoazasterol-mediated inhibition of yeast sterol biosynthesis. J Bacteriol. 1976 Dec;128(3):730–734. doi: 10.1128/jb.128.3.730-734.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Botsford J. L., Jr, Parks L. W. Serine transhydroxymethylase in methionine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1969 Mar;97(3):1176–1183. doi: 10.1128/jb.97.3.1176-1183.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DULANEY E. L., STAPLEY E. O., SIMPF K. Studies on ergosterol production by yeasts. Appl Microbiol. 1954 Nov;2(6):371–379. doi: 10.1128/am.2.6.371-379.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Monner D. A., Parks L. W. A method for extraction of sterols from enzymically active cell-free preparations. Anal Biochem. 1968 Oct 24;25(1):61–69. doi: 10.1016/0003-2697(68)90081-x. [DOI] [PubMed] [Google Scholar]
- Parks L. W., Anding C., Ourisson G. Sterol transmethylation during aerobic adaptation of yeast. Eur J Biochem. 1974 Apr 16;43(3):451–458. doi: 10.1111/j.1432-1033.1974.tb03431.x. [DOI] [PubMed] [Google Scholar]
- Thompson E. D., Parks L. W. Effect of altered sterol composition on growth characteristics of Saccharomyces cerevisiae. J Bacteriol. 1974 Nov;120(2):779–784. doi: 10.1128/jb.120.2.779-784.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]