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. 1977 Mar;129(3):1424–1427. doi: 10.1128/jb.129.3.1424-1427.1977

Host cell and ultraviolet reactivation of ultraviolet-irradiated Mycoplasmaviruses.

J Das, J A Nowak, J Maniloff
PMCID: PMC235118  PMID: 845120

Abstract

The mycoplasma Acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (UV) reactivation of UV-irradiated mycoplasmaviruses. Host cell reactivation was examined by comparing the survival abilities of UV-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untreated and on acriflavine-treated cells. Acriflavine treatment inhibited cell exision repair. Decreased survival on the acriflavine-treated cells demonstrated host cell reactivation. UV reactivation was studied by comparing the survival of UV-irradiated virus plated on untreated cells with its survival on cells that received a small UV dose before plating. The UV-irradiated cells gave increased virus survival, showing UV reactivation. Similar experiments with a single-stranded deoxyribonucleic acid mycoplasmavirus showed that this virus could be UV reactivated, but not host cell reactivated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bak A. L., Black F. T., Christiansen C., Freundt E. A. Genome size of mycoplasmal DNA. Nature. 1969 Dec 20;224(5225):1209–1210. doi: 10.1038/2241209a0. [DOI] [PubMed] [Google Scholar]
  2. Blanco M., Devoret R. Repair mechanisms involved in prophage reactivation and UV reactivation of UV-irradiated phage lambda. Mutat Res. 1973 Mar;17(3):293–305. doi: 10.1016/0027-5107(73)90001-8. [DOI] [PubMed] [Google Scholar]
  3. Boyle J. M., Setlow R. B. Correlations between host-cell reactivation, ultraviolet reactivation and pyrimidine dimer excision in the DNA of bacteriophage lambda. J Mol Biol. 1970 Jul 14;51(1):131–144. doi: 10.1016/0022-2836(70)90275-5. [DOI] [PubMed] [Google Scholar]
  4. Das J., Maniloff J., Bhattacharjee S. B. Dark and light repair in ultraviolet-irradiated Acholeplasma laidlawii. Biochim Biophys Acta. 1972 Jan 31;259(2):189–197. doi: 10.1016/0005-2787(72)90058-5. [DOI] [PubMed] [Google Scholar]
  5. FEINER R. R., HILL R. F. EFFECT OF BASIC DYES ON HOST-CELL REACTIVATION OF ULTRA-VIOLETIRRADIATED PHAGE. Nature. 1963 Oct 19;200:291–293. doi: 10.1038/200291a0. [DOI] [PubMed] [Google Scholar]
  6. Folsome C. E. Deoxyribonucleate binding and transformation in Mycoplasma laidlawii. J Gen Microbiol. 1968 Jan;50(1):43–53. doi: 10.1099/00221287-50-1-43. [DOI] [PubMed] [Google Scholar]
  7. GAREN A., ZINDER N. D. Radiological evidence for partial genetic homology between bacteriophage and host bacteria. Virology. 1955 Nov;1(4):347–376. doi: 10.1016/0042-6822(55)90030-1. [DOI] [PubMed] [Google Scholar]
  8. Garwes D. J., Pike B. V., Wyld S. G., Pocock D. H., Gourlay R. N. Characterization of Mycoplasmatales virus-laidlawii 3. J Gen Virol. 1975 Oct;29(1):11–24. doi: 10.1099/0022-1317-29-1-11. [DOI] [PubMed] [Google Scholar]
  9. Gourlay R. N. Mycoplasmatales virus-laidlawii 2, a new virus isolated from Acholeplasma laidlawii. J Gen Virol. 1971 Jul;12(1):65–67. doi: 10.1099/0022-1317-12-1-65. [DOI] [PubMed] [Google Scholar]
  10. HARM W. On the relationship between host-cell reactivation and UV-reactivation in UV-inactivated phages. Z Vererbungsl. 1963;94:67–79. doi: 10.1007/BF00895157. [DOI] [PubMed] [Google Scholar]
  11. JAGGER J. Photoreactivation. Bacteriol Rev. 1958 Jun;22(2):99–142. doi: 10.1128/br.22.2.99-142.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Liss A., Maniloff J. Infection of Acholeplasma laidlawii by MVL51 virus. Virology. 1973 Sep;55(1):118–126. doi: 10.1016/s0042-6822(73)81013-x. [DOI] [PubMed] [Google Scholar]
  13. Liss A., Maniloff J. Isolation of Mycoplasmatales viruses and characterization of MVL1, MVL52, and MVG51. Science. 1971 Aug 20;173(3998):725–727. doi: 10.1126/science.173.3998.725. [DOI] [PubMed] [Google Scholar]
  14. Liss A., Maniloff J. Transfection mediated by Mycoplasmatales viral DNA. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3423–3427. doi: 10.1073/pnas.69.11.3423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maniloff J., Das J., Christensen J. R. Viruses of mycoplasmas and spiroplasmas. Adv Virus Res. 1977;21:343–380. doi: 10.1016/s0065-3527(08)60765-4. [DOI] [PubMed] [Google Scholar]
  16. Maniloff J., Morowitz H. J. Cell biology of the mycoplasmas. Bacteriol Rev. 1972 Sep;36(3):263–290. doi: 10.1128/br.36.3.263-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Neimark H. C. Division of mycoplasmas into subgroups. J Gen Microbiol. 1970 Oct;63(2):249–263. doi: 10.1099/00221287-63-2-249. [DOI] [PubMed] [Google Scholar]
  18. SETLOW R. B. PHYSICAL CHANGES AND MUTAGENESIS. J Cell Physiol. 1964 Oct;64:SUPPL 1–1:68. [PubMed] [Google Scholar]
  19. Smith D. W., Hanawalt P. C. Repair replication of DNA in ultraviolet irradiated Mycoplasma laidlawii B. J Mol Biol. 1969 Nov 28;46(1):57–72. doi: 10.1016/0022-2836(69)90057-6. [DOI] [PubMed] [Google Scholar]
  20. WITKIN E. M. Modification of mutagenesis initiated by ultraviolet light through postteatment of bacteria with basic dyes. J Cell Comp Physiol. 1961 Dec;58(3):135–144. doi: 10.1002/jcp.1030580413. [DOI] [PubMed] [Google Scholar]
  21. Weigle J. J. Induction of Mutations in a Bacterial Virus. Proc Natl Acad Sci U S A. 1953 Jul;39(7):628–636. doi: 10.1073/pnas.39.7.628. [DOI] [PMC free article] [PubMed] [Google Scholar]

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