Abstract
From strains of Escherichia coli that carry deletions of the trp region, five different mutants were isolated that were capable of synthesizing tryptophanase at unusually high rates in conditions of severe catabolite repression. Notwithstanding the comparative insensitivity to catabolite repression, the rates of tryptophanase synthesis in the mutants were greatly diminished by the introduction of a defective gene for adenyl cyclase. Each of the mutants segregated variants of the parental type. The results of genetic analysis appear to be consistent with the mutants arose by duplication of the tryptophanase gene.
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Selected References
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- Anderson R. P., Miller C. G., Roth J. R. Tandem duplications of the histidine operon observed following generalized transduction in Salmonella typhimurium. J Mol Biol. 1976 Aug 5;105(2):201–218. doi: 10.1016/0022-2836(76)90107-8. [DOI] [PubMed] [Google Scholar]
- Black J. A., Dixon G. H. Amino-acid sequence of alpha chains of human haptoglobins. Nature. 1968 May 25;218(5143):736–741. doi: 10.1038/218736a0. [DOI] [PubMed] [Google Scholar]
- Cantor C. R., Jukes T. H. The repetition of homologous sequences in the polypetide chains of certain cytochromes and globins. Proc Natl Acad Sci U S A. 1966 Jul;56(1):177–184. doi: 10.1073/pnas.56.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glansdorff N., Sand G. Duplication of a gene belonging to an arginine operon of Escherichia coli K-12. Genetics. 1968 Oct;60(2):257–268. doi: 10.1093/genetics/60.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill C. W., Foulds J., Soll L., Berg P. Instability of a missense suppressor resulting from a duplication of genetic material. J Mol Biol. 1969 Feb 14;39(3):563–581. doi: 10.1016/0022-2836(69)90146-6. [DOI] [PubMed] [Google Scholar]
- Jackson E. N., Yanofsky C. Duplication-translocations of tryptophan operon genes in Escherichia coli. J Bacteriol. 1973 Oct;116(1):33–40. doi: 10.1128/jb.116.1.33-40.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reznikoff W. S., Michels C. A., Cooper T. G., Silverstone A. E., Magasanik B. Inhibition of lacZ gene translation initiation in trp-lac fusion strains. J Bacteriol. 1974 Mar;117(3):1231–1239. doi: 10.1128/jb.117.3.1231-1239.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reznikoff W. S., Miller J. H., Scaife J. G., Beckwith J. R. A mechanism for repressor action. J Mol Biol. 1969 Jul 14;43(1):201–213. doi: 10.1016/0022-2836(69)90089-8. [DOI] [PubMed] [Google Scholar]
- Ward D. F., Yudkin M. D. Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Tryptophanase promoter-like mutations. J Gen Microbiol. 1976 Jan;92(1):133–137. doi: 10.1099/00221287-92-1-133. [DOI] [PubMed] [Google Scholar]
- Yudkin M. D. Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Mutations distant from the tryptophanase gene. J Gen Microbiol. 1976 Jan;92(1):125–132. doi: 10.1099/00221287-92-1-125. [DOI] [PubMed] [Google Scholar]