Abstract
The incorporation of [14C]acetate into the fatty acid moieties of lipid A was measured during amino acid starvation of rel+ and relA strains of Escherichia coli K-12. The synthesis of the beta-hydroxymyristate and other fatty acid moieties was inhibited two- to fourfold in rel+ strains, whereas no inhibition was observed in relA strains. The fatty acid compositions of the phospholipids synthesized after amino acid starvation or rel+ and relA strains were also determined.
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Selected References
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- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- BURTON A. J., CARTER H. E. PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI. Biochemistry. 1964 Mar;3:411–418. doi: 10.1021/bi00891a018. [DOI] [PubMed] [Google Scholar]
- Bell R. M. Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant. J Bacteriol. 1974 Mar;117(3):1065–1076. doi: 10.1128/jb.117.3.1065-1076.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cashel M. Regulation of bacterial ppGpp and pppGpp. Annu Rev Microbiol. 1975;29:301–318. doi: 10.1146/annurev.mi.29.100175.001505. [DOI] [PubMed] [Google Scholar]
- Cronan J. E., Jr, Wulff D. L. A role for phospholipid hydrolysis in the lysis of Escherichia coli infected with bacteriophage T4. Virology. 1969 Jun;38(2):241–246. doi: 10.1016/0042-6822(69)90365-1. [DOI] [PubMed] [Google Scholar]
- Gelmann E. P., Cronan J. E., Jr Mutant of Escherichia coli deficient in the synthesis of cis-vaccenic acid. J Bacteriol. 1972 Oct;112(1):381–387. doi: 10.1128/jb.112.1.381-387.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Golden N. G., Powell G. L. Stringent and relaxed control of phospholipid metabolism in Escherichia coli. J Biol Chem. 1972 Oct 25;247(20):6651–6658. [PubMed] [Google Scholar]
- Ishiguro E. E., Ramey W. D. Stringent control of peptidoglycan biosynthesis in Escherichia coli K-12. J Bacteriol. 1976 Sep;127(3):1119–1126. doi: 10.1128/jb.127.3.1119-1126.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marinari L. A., Goldfine H., Panos C. Specificity of cyclopropane fatty acid synthesis in Escherichia coli. Utilization of isomers of monounsaturated fatty acids. Biochemistry. 1974 Apr 23;13(9):1978–1983. doi: 10.1021/bi00706a030. [DOI] [PubMed] [Google Scholar]
- Merlie J. P., Pizer L. I. Regulation of phospholipid synthesis in Escherichia coli by guanosine tetraphosphate. J Bacteriol. 1973 Oct;116(1):355–366. doi: 10.1128/jb.116.1.355-366.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nunn W. D., Cronan J. E., Jr Evidence for a direct effect on fatty acid synthesis in rela gene control of membrane phospholipid synthesis. J Mol Biol. 1976 Mar 25;102(1):167–172. doi: 10.1016/0022-2836(76)90080-2. [DOI] [PubMed] [Google Scholar]
- Nunn W. D., Cronan J. E., Jr Regulation of membrane phospholipid synthesis by the relA gene: dependence on ppGpp levels. Biochemistry. 1976 Jun 15;15(12):2546–2550. doi: 10.1021/bi00657a009. [DOI] [PubMed] [Google Scholar]
- Nunn W. D., Cronan J. E., Jr rel Gene control of lipid synthesis in Escherichia coli. Evidence for eliminating fatty acid synthesis as the sole regulatory site. J Biol Chem. 1974 Jun 25;249(12):3994–3996. [PubMed] [Google Scholar]
- Osborn M. J., Rick P. D., Lehmann V., Rupprecht E., Singh M. Structure and biogenesis of the cell envelope of gram-negative bacteria. Ann N Y Acad Sci. 1974 May 10;235(0):52–65. doi: 10.1111/j.1749-6632.1974.tb43256.x. [DOI] [PubMed] [Google Scholar]
- Polakis S. E., Guchhait R. B., Lane M. D. Stringent control of fatty acid synthesis in Escherichia coli. Possible regulation of acetyl coenzyme A carboxylase by ppGpp. J Biol Chem. 1973 Nov 25;248(22):7957–7966. [PubMed] [Google Scholar]
- Rooney S. A., Goldfine H. Isolation and characterization of 2-keto-3-deoxyoctonate-lipid A from a heptose-deficient mutant of Escherichia coli. J Bacteriol. 1972 Aug;111(2):531–541. doi: 10.1128/jb.111.2.531-541.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rooney S. A., Goldfine H., Sweeley C. C. The identification of trans-2-tetradecenoic acid in hydrolysates of lipid A from Escherichia coli. Biochim Biophys Acta. 1972 Jul 7;270(3):289–295. doi: 10.1016/0005-2760(72)90192-0. [DOI] [PubMed] [Google Scholar]
- Sokawa Y., Nakao E., Kaziro Y. On the nature of the control by RC gene in e. coli: amino acid-dependent control of lipid synthesis. Biochem Biophys Res Commun. 1968 Oct 10;33(1):108–112. doi: 10.1016/0006-291x(68)90263-5. [DOI] [PubMed] [Google Scholar]
- Stein J. P., Jr, Bloch K. E. Inhibition of E. coli beta-hydroxydecanoyl thioester dehydrase by ppGpp. Biochem Biophys Res Commun. 1976 Dec 20;73(4):881–884. doi: 10.1016/0006-291x(76)90204-7. [DOI] [PubMed] [Google Scholar]
- Tropp B. E., Meade L. C., Thomas P. J. Consequences of expression of the "relaxed" genotype of the RC gene. Lipid synthesis. J Biol Chem. 1970 Feb 25;245(4):855–858. [PubMed] [Google Scholar]
