Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Apr;130(1):292–296. doi: 10.1128/jb.130.1.292-296.1977

Effects of carbon dioxide, urea, and ammonia on growth of Ureaplasma urealyticum (T-strain mycoplasma).

G K Masover, S Razin, L Hayflick
PMCID: PMC235205  PMID: 15979

Abstract

By use of a simple device for continuous CO2 gassing of Ureaplasma urealyticum cultures growing in a liquid medium, we have been able to separate some of the effects of urea, CO2, ammonia, and pH on growth. The CO2 acted as a superior buffer in the pH range 5.7 to 6.8, which is optimal for Ureaplasma growth. It was, therefore, possible to observe the effect of repeated additions of urea to the culture without alkalinization of the growth medium. We found that the repeated additions of urea did not enhance Ureaplasma growth, and the resultant accumulation of ammonium ions (greater than 2,000 microng/ml) did not cause more rapid death under these conditions. By abruptly changing the gaseous environment from CO2 to N2, it was possible to cause a rapid pH change in the culture to a value above 8.0. This resulted in a more rapid death of the organisms.

Full text

PDF
292

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ford D. K., MacDonald J. Influence of urea on the growth of T-strain mycoplasmas. J Bacteriol. 1967 May;93(5):1509–1512. doi: 10.1128/jb.93.5.1509-1512.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ford D. K., McCandlish K. L., Gronlund A. F. Metabolism of 14C-urea by T-strain mycoplasma. J Bacteriol. 1970 May;102(2):605–606. doi: 10.1128/jb.102.2.605-606.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Furness G. T-mycoplasmas: their growth and production of a toxic substance in broth. J Infect Dis. 1973 Jan;127(1):9–16. doi: 10.1093/infdis/127.1.9. [DOI] [PubMed] [Google Scholar]
  4. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  5. Hendley J. O., Allred E. N. Prolonged stationary-phase growth of T-strain mycoplasmas in broth culture. Infect Immun. 1972 Feb;5(2):164–168. doi: 10.1128/iai.5.2.164-168.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Manchee R. J., Taylor-Robinson D. Enhanced growth of T-strain mycoplasmas with N-2-hydroxyethylpiperazone-N'-2-ethanesulfonic acid buffer. J Bacteriol. 1969 Oct;100(1):78–85. doi: 10.1128/jb.100.1.78-85.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Masover G. K., Benson J. R., Hayflick L. Growth of T-strain mycoplasmas in medium without added urea: effect of trace amounts of urea and of a urease inhibitor. J Bacteriol. 1974 Feb;117(2):765–774. doi: 10.1128/jb.117.2.765-774.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Masover G. K., Hayflick L. Dialysis culture of T-strain mycoplasmas. J Bacteriol. 1974 Apr;118(1):46–52. doi: 10.1128/jb.118.1.46-52.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Masover G. K., Razin S., Hayflick L. Localization of enzymes in Ureaplasma urealyticum (T-strain mycoplasma). J Bacteriol. 1977 Apr;130(1):297–302. doi: 10.1128/jb.130.1.297-302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Masover G. K., Sawyer J. E., Hayflick L. Urea-hydrolyzing activity of a T-strain mycoplasma: Ureaplasma urealyticum. J Bacteriol. 1976 Feb;125(2):581–587. doi: 10.1128/jb.125.2.581-587.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Purcell R. H., Taylor-Robinson D., Wong D., Chanock R. M. Color test for the measurement of antibody to T-strain mycoplasmas. J Bacteriol. 1966 Jul;92(1):6–12. doi: 10.1128/jb.92.1.6-12.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Razin S., Masover G. K., Palant M., Hayflick L. Morphology of Ureaplasma urealyticum (T-mycoplasma) organisms and colonies. J Bacteriol. 1977 Apr;130(1):464–471. doi: 10.1128/jb.130.1.464-471.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Romano N., Ajello F., Massenti M. F., Scarlata G. Effect of L-histidine and various buffers on colony size and morphology of a T-strain of mycoplasma. Boll Ist Sieroter Milan. 1975 Oct 20;54(4):292–295. [PubMed] [Google Scholar]
  14. SELIGSON D., SELIGSON H. A microdiffusion method for the determination of nitrogen liberated as ammonia. J Lab Clin Med. 1951 Aug;38(2):324–330. [PubMed] [Google Scholar]
  15. SHEPARD M. C., LUNCEFORD C. D. EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. J Bacteriol. 1965 Feb;89:265–270. doi: 10.1128/jb.89.2.265-270.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shepard M. C. Differential methods for identification of T-mycoplasmas based on demonstration of urease. J Infect Dis. 1973 Mar;127(Suppl):S22–S25. doi: 10.1093/infdis/127.supplement_1.s22. [DOI] [PubMed] [Google Scholar]
  17. Shepard M. C. Human mycoplasma infections. Health Lab Sci. 1966 Jul;3(3):163–169. [PubMed] [Google Scholar]
  18. Shepard M. C., Lunceford C. D. Occurrence of urease in T strains of Mycoplasma. J Bacteriol. 1967 May;93(5):1513–1520. doi: 10.1128/jb.93.5.1513-1520.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Windsor G. D., Trigwell J. A. Prolonged survival of Ureaplasma urealyticum in liquid culture. J Med Microbiol. 1976 Feb;9(1):101–103. doi: 10.1099/00222615-9-1-101. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES