Abstract
Five methods were employed to determine the heterogeneity or homogeneity of lipopolysaccharides from four acholeplasmal species, Acholeplasma axanthum, A. granularum, A. laidlawii, and A. modicum. A axanthum lipopolysaccharide behaved as a single component in all tests. A. granularum exhibited two components of identical composition and antigenic specificity. A. modicum lipopolysaccharide behaved as three components in two tests, but all three were similar in composition and identical serologically. The separable components of lipopolysaccharides from A. granularum and A. modicum probably represent size differences only. A. laidlwii lipopolysaccharide contained two distinct components by all methods. One was identified as the previously reported amino sugar polymer, whereas the other was a lipopolysaccharide containing both neutral and amino sugars.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
- Gilliam J. M., Morowitz H. J. Characterization of the plasma membrane of Mycoplasma laidlawii. IX. Isolation and characterization of the membrane polyhexosamine. Biochim Biophys Acta. 1972 Aug 9;274(2):353–363. doi: 10.1016/0005-2736(72)90183-6. [DOI] [PubMed] [Google Scholar]
- Jann B., Reske K., Jann K. Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Eur J Biochem. 1975 Dec 1;60(1):239–246. doi: 10.1111/j.1432-1033.1975.tb20996.x. [DOI] [PubMed] [Google Scholar]
- Mayberry-Carson K. J., Langworthy T. A., MAyberry W. R., Smith P. F. A new class of lipopolysaccharide from Thermoplasma acidophilum. Biochim Biophys Acta. 1974 Aug 22;360(2):217–229. doi: 10.1016/0005-2760(74)90171-4. [DOI] [PubMed] [Google Scholar]
- Mayberry-Carson K. J., Roth I. L., Smith P. F. Ultrastructure of lipopolysaccharide isolated from Thermoplasma acidophilum. J Bacteriol. 1975 Feb;121(2):700–703. doi: 10.1128/jb.121.2.700-703.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayberry W. R., Smith P. F., Langworthy T. A. Heptose-containing pentaglycosyl diglyceride among the lipids of Acholeplasma modicum. J Bacteriol. 1974 Jun;118(3):898–904. doi: 10.1128/jb.118.3.898-904.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. F., Langworthy T. A., Mayberry W. R. Distribution and composition of lipopolysaccharides from mycoplasmas. J Bacteriol. 1976 Mar;125(3):916–922. doi: 10.1128/jb.125.3.916-922.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugiyama T., Smith P. F., Langworthy T. A., Mayberry W. R. Immunological analysis of glycolipids and lipopolysaccharides derived from various mycoplasmas. Infect Immun. 1974 Dec;10(6):1273–1279. doi: 10.1128/iai.10.6.1273-1279.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]