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. 1977 May;130(2):766–774. doi: 10.1128/jb.130.2.766-774.1977

Recovery of Saccharomyces cerevisiae mating-type a cells from G1 arrest by alpha factor.

R K Chan
PMCID: PMC235279  PMID: 400792

Abstract

Mating-type a cells of the yeast Saccharomyces cerevisiae that had been specifically arrested in the G1 phase of the cell cycle by alpha factor, an oligopeptide pheromone made by alpha cells, recovered and resumed cell division after a period of inhibition which was dependent on the concentration of alpha factor used. These treated a cells were more resistant to alpha factor than untreated a cells, but lost their resistance upon further cell division. However, cells arrested for 6 h were no more resistant to alpha factor than cells arrested for only 2.5 h. Mating-type a strains could inactivate or remove alpha factor from the culture fluid, but two a sterile (nonmating) mutants and an a/alpha diploid strain could not. These results suggest that a cells have a mechanism, which may involve uptake or inactivation of alpha factor, for recovering from alpha factor arrest. However, the results do not distinguish between a recovery mechanism which is constitutive and one which is induced by alpha factor. The loss of alpha factor activity during recovery appeared to be primarily cell contact mediated, although an extracellular, diffusible inhibitor of alpha factor that is labile or that functions stoichiometrically could not be ruled out.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res. 1973 Jan;76(1):99–110. doi: 10.1016/0014-4827(73)90424-2. [DOI] [PubMed] [Google Scholar]
  2. Duntze W., MacKay V., Manney T. R. Saccharomyces cerevisiae: a diffusible sex factor. Science. 1970 Jun 19;168(3938):1472–1473. doi: 10.1126/science.168.3938.1472. [DOI] [PubMed] [Google Scholar]
  3. Duntze W., Stötzler D., Bücking-Throm E., Kalbitzer S. Purification and partial characterization of -factor, a mating-type specific inhibitor of cell reproduction from Saccharomyces cerevisiae. Eur J Biochem. 1973 Jun;35(2):357–365. doi: 10.1111/j.1432-1033.1973.tb02847.x. [DOI] [PubMed] [Google Scholar]
  4. Gooday G. W. Fungal sex hormones. Annu Rev Biochem. 1974;43(0):35–87. doi: 10.1146/annurev.bi.43.070174.000343. [DOI] [PubMed] [Google Scholar]
  5. Hartwell L. H. Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures. J Bacteriol. 1970 Dec;104(3):1280–1285. doi: 10.1128/jb.104.3.1280-1285.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hicks J. B., Herskowitz I. Evidence for a new diffusible element of mating pheromones in yeast. Nature. 1976 Mar 18;260(5548):246–248. doi: 10.1038/260246a0. [DOI] [PubMed] [Google Scholar]
  7. Knights E. B., Baylin S. B., Foster G. V. Control of polypeptide hormones by enzymatic degradation. Lancet. 1973 Sep 29;2(7831):719–723. doi: 10.1016/s0140-6736(73)92550-6. [DOI] [PubMed] [Google Scholar]
  8. Lindegren C. C., Lindegren G. A New Method for Hybridizing Yeast. Proc Natl Acad Sci U S A. 1943 Oct 15;29(10):306–308. doi: 10.1073/pnas.29.10.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Machlis L. The chemotactic activity of various sirenins and analogues and the uptake of sirenin by the sperm of allomyces. Plant Physiol. 1973 Dec;52(6):527–530. doi: 10.1104/pp.52.6.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mackay V., Manney T. R. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants. Genetics. 1974 Feb;76(2):255–271. doi: 10.1093/genetics/76.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mackay V., Manney T. R. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of nonmating mutants. Genetics. 1974 Feb;76(2):273–288. doi: 10.1093/genetics/76.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stötzler D., Duntze W. Isolation and characterization of four related peptides exhibiting alpha factor activity from Saccharomyces cerevisiae. Eur J Biochem. 1976 May 17;65(1):257–262. doi: 10.1111/j.1432-1033.1976.tb10412.x. [DOI] [PubMed] [Google Scholar]
  13. Throm E., Duntze W. Mating-Type-Dependent Inhibition of Deoxyribonucleic Acid Synthesis in Saccharomyces cerevisiae. J Bacteriol. 1970 Dec;104(3):1388–1390. doi: 10.1128/jb.104.3.1388-1390.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilkinson L. E., Pringle J. R. Transient G1 arrest of S. cerevisiae cells of mating type alpha by a factor produced by cells of mating type a. Exp Cell Res. 1974 Nov;89(1):175–187. doi: 10.1016/0014-4827(74)90200-6. [DOI] [PubMed] [Google Scholar]

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