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. 1977 Jun;130(3):1064–1071. doi: 10.1128/jb.130.3.1064-1071.1977

Genetic loci of hemolysin production in Streptococcus faecalis subsp. zymogenes.

M L Frazier, L N Zimmerman
PMCID: PMC235328  PMID: 405371

Abstract

Two plasmids corresponding to molecular weights of 38.5 X 10(6) and 3.6 X 10(6) have been identified in Streptococcus faecalis subsp. zymogenes strain X-14. The larger plasmid is required for hemolysin-bacteriolysin production. Strain L2, a nonlytic nitrosoquanidine mutant of strain X-14, still harbors the hemolytic plasmid and produces the lysin component, but not the activator component, of the lytic system. Conjugal transfer of this plasmid from strain L2 to plasmid-free strains and strains cured of the 38.5-megadalton plasmid gives rise to hemolytic recipients. This implicates a gene in hemolysin production at a site other than the 38.5-megadalton plasmid.

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Selected References

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  1. Appelbaum B., Zimmerman L. N. Production of Hemolysin and Bacteriolysin in a Synthetic Medium by Streptococcus faecalis var. zymogenes. Infect Immun. 1974 Nov;10(5):991–995. doi: 10.1128/iai.10.5.991-995.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROCK T. D., DAVIE J. M. PROBABLE IDENTITY OF A GROUP D HEMOLYSIN WITH A BACTERIOCINE. J Bacteriol. 1963 Oct;86:708–712. doi: 10.1128/jb.86.4.708-712.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROCK T. D. HOST RANGE OF CERTAIN VIRULENT AND TEMPERATE BACTERIOPHAGES ATTACKING GROUP D STREPTOCOCCI. J Bacteriol. 1964 Jul;88:165–171. doi: 10.1128/jb.88.1.165-171.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BROCK T. D., PEACHER B., PIERSON D. SURVEY OF THE BACTERIOCINES OF ENTEROCOCCI. J Bacteriol. 1963 Oct;86:702–707. doi: 10.1128/jb.86.4.702-707.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Basinger S. F., Jackson R. W. Bacteriocin (hemolysin) of Streptococcus zymogenes. J Bacteriol. 1968 Dec;96(6):1895–1902. doi: 10.1128/jb.96.6.1895-1902.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Baxter-Gabbard K. L. A simple method for the large-scale preparation of sucrose gradients. FEBS Lett. 1972 Jan 15;20(1):117–119. doi: 10.1016/0014-5793(72)80031-0. [DOI] [PubMed] [Google Scholar]
  8. Bazaral M., Helinski D. R. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513–3520. doi: 10.1021/bi00850a028. [DOI] [PubMed] [Google Scholar]
  9. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  10. Clewell D. B., Yagi Y., Dunny G. M., Schultz S. K. Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J Bacteriol. 1974 Jan;117(1):283–289. doi: 10.1128/jb.117.1.283-289.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Davie J. M., Brock T. D. Effect of teichoic acid on resistance to the membrane-lytic agent of Streptococcus zymogenes. J Bacteriol. 1966 Dec;92(6):1623–1631. doi: 10.1128/jb.92.6.1623-1631.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dunny G. M., Clewell D. B. Transmissible toxin (hemolysin) plasmid in Streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid. J Bacteriol. 1975 Nov;124(2):784–790. doi: 10.1128/jb.124.2.784-790.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Granato P. A., Jackson R. W. Bicomponent nature of lysin from Streptococcus zymogenes. J Bacteriol. 1969 Nov;100(2):865–868. doi: 10.1128/jb.100.2.865-868.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jackson R. W. Bacteriolysis and inhibition of gram-positive bacteria by components of Streptococcus zymogenes lysin. J Bacteriol. 1971 Jan;105(1):156–159. doi: 10.1128/jb.105.1.156-159.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jacob A. E., Douglas G. J., Hobbs S. J. Self-transferable plasmids determining the hemolysin and bacteriocin of Streptococcus faecalis var. zymogenes. J Bacteriol. 1975 Mar;121(3):863–872. doi: 10.1128/jb.121.3.863-872.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jacob A. E., Hobbs S. J. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. doi: 10.1128/jb.117.2.360-372.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tomura T., Hirano T., Ito T., Yoshioka M. Transmission of bacteriocinogenicity by conjugation in group D streptococci. Jpn J Microbiol. 1973 Nov;17(6):445–452. doi: 10.1111/j.1348-0421.1973.tb00930.x. [DOI] [PubMed] [Google Scholar]
  18. Werth J. M., Jackson R. W. Effects of various drugs on hemolytic activity of Streptococcus zymogenes. J Bacteriol. 1971 Nov;108(2):844–848. doi: 10.1128/jb.108.2.844-848.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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