Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Jun;130(3):1144–1148. doi: 10.1128/jb.130.3.1144-1148.1977

Unsaturated fatty acid mutants of Neurospora crassa.

W A Scott
PMCID: PMC235338  PMID: 140860

Abstract

Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type were assigned to two complementation classes, ufa-1 and ufa-2, located on linkage group V. Unsaturated fatty acids with varying degrees of unsaturation, chain length, and double-bond position as well as different steric configurations were tested for growth requirements.

Full text

PDF
1144

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CATCHESIDE D. G. Isolation of nutritional mutants of Neurospora crassa by filtration enrichment. J Gen Microbiol. 1954 Aug;11(1):34–36. doi: 10.1099/00221287-11-1-34. [DOI] [PubMed] [Google Scholar]
  2. Harder M. E., Beacham I. R., Cronan J. E., Jr, Beacham K., Honegger J. L., Silbert D. F. Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3105–3109. doi: 10.1073/pnas.69.11.3105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Henry S. A., Fogel S. Saturated fatty acid mutants in yeast. Mol Gen Genet. 1971;113(1):1–19. doi: 10.1007/BF00335003. [DOI] [PubMed] [Google Scholar]
  4. Henry S. A., Keith A. D. Saturated fatty acid requirer of Neurospora crassa. J Bacteriol. 1971 Apr;106(1):174–182. doi: 10.1128/jb.106.1.174-182.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LEIN J., LEIN P. S. Studies on a Neurospora mutant requiring unsaturated fatty acids for growth. J Bacteriol. 1949 Nov;58(5):595–599. doi: 10.1128/jb.58.5.595-599.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LESTER H. E., GROSS S. R. Efficient method for selection of auxotrophic mutants of Neurospora. Science. 1959 Feb 27;129(3348):572–572. doi: 10.1126/science.129.3348.572. [DOI] [PubMed] [Google Scholar]
  7. Malling H. V., De Serres F. J. Genetic effects of N-methyl-N'-nitro-N-nitrosoguanidine in Neurospora crassa. Mol Gen Genet. 1970;106(3):195–207. doi: 10.1007/BF00340379. [DOI] [PubMed] [Google Scholar]
  8. Perkins D. D., Newmeyer D., Taylor C. W., Bennett D. C. New markers and map sequences in Neurospora crassa, with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica. 1969;40(3):247–278. doi: 10.1007/BF01787357. [DOI] [PubMed] [Google Scholar]
  9. Resnick M. A., Mortimer R. K. Unsaturated fatty acid mutants of Saccharomyces cerevisiae. J Bacteriol. 1966 Sep;92(3):597–600. doi: 10.1128/jb.92.3.597-600.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schweizer E., Kühn L., Castorph H. A new gene cluster in yeast: the fatty acid synthetase system. Hoppe Seylers Z Physiol Chem. 1971 Mar;352(3):377–384. doi: 10.1515/bchm2.1971.352.1.377. [DOI] [PubMed] [Google Scholar]
  11. Silbert D. F. Genetic modification of membrane lipid. Annu Rev Biochem. 1975;44:315–339. doi: 10.1146/annurev.bi.44.070175.001531. [DOI] [PubMed] [Google Scholar]
  12. Silbert D. F., Ruch F., Vagelos P. R. Fatty acid replacements in a fatty acid auxotroph of Escherichia coli. J Bacteriol. 1968 May;95(5):1658–1665. doi: 10.1128/jb.95.5.1658-1665.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Silbert D. F., Vagelos P. R. Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1579–1586. doi: 10.1073/pnas.58.4.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wisnieski B. J., Kiyomoto R. K. Fatty acid desaturase mutants of yeast: growth requirements and electron spin resonance spin-label distribution. J Bacteriol. 1972 Jan;109(1):186–195. doi: 10.1128/jb.109.1.186-195.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES