Abstract
The chemical structure of the surface polysaccharide from Staphylococcus aureus M was investigated by a combination of methanolytic, hydrolytic, and chromatographic techniques. The repeating unit that was most consistent with the data was a hexasaccharide composed of N-acetyl-D-aminogalacturonic acid, N-acetyl-D-fucosamine, and taurine in molar ratios of 4:2:1. A disaccharide was isolated and characterized, by combined gas-liquid chromatography-mass spectrometry, as N-acetyl-D-aminogalacturonyl-(1 leads to 3)-N-acetyl-D-fucosamine. Taurine is linked to a carboxyl group of N-acetyl-D-aminogalacturonic acid via an amide bond.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HANESSIAN S., HASKELL T. H. STRUCTURAL STUDIES ON STAPHYLOCOCCAL POLYSACCHARIDE ANTIGEN. J Biol Chem. 1964 Sep;239:2758–2764. [PubMed] [Google Scholar]
- LUDOWIEG J., DORFMAN A. A micromethod for the colorimetric determination of N-acetyl groups in acid mucopolysaccharides. Biochim Biophys Acta. 1960 Feb 26;38:212–218. doi: 10.1016/0006-3002(60)91233-6. [DOI] [PubMed] [Google Scholar]
- Liau D. F., Melly M. A., Hash J. H. Surface polysaccharide from Staphylococcus aureus M that contains taurine, D-aminogalacturonic acid, and D-fucosamine. J Bacteriol. 1974 Sep;119(3):913–922. doi: 10.1128/jb.119.3.913-922.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RONDLE C. J., MORGAN W. T. The determination of glucosamine and galactosamine. Biochem J. 1955 Dec;61(4):586–589. doi: 10.1042/bj0610586. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sandford P. A., Watson P. R., Jeanes A. R. An extracellular microbial polysaccharide composed of 2-acetamido-2-deoxy-D-glucose and 2-acetamido-2-deoxy-D-glucuronic acid: radiochemical and gas-chromatographic analysis of the products of methanolysis. Carbohydr Res. 1973 Jul;29(1):153–164. doi: 10.1016/s0008-6215(00)82079-0. [DOI] [PubMed] [Google Scholar]
- Seiler N. Use of the dansyl reaction in biochemical analysis. Methods Biochem Anal. 1970;18:259–337. doi: 10.1002/9780470110362.ch5. [DOI] [PubMed] [Google Scholar]
- Shih J. W., Hash J. H. The N,O-diacetylmuramidase of Chalaropsis species. 3. Amino acid composition and partial structural formula. J Biol Chem. 1971 Feb 25;246(4):994–1006. [PubMed] [Google Scholar]
- Taylor R. L., Conrad H. E. Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups. Biochemistry. 1972 Apr 11;11(8):1383–1388. doi: 10.1021/bi00758a009. [DOI] [PubMed] [Google Scholar]
- Wu T. C., Park J. T. Chemical characterization of a new surface antigenic polysaccharide from a mutant of Staphylococcus aureus. J Bacteriol. 1971 Nov;108(2):874–884. doi: 10.1128/jb.108.2.874-884.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]