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. 1977 Jul;131(1):214–223. doi: 10.1128/jb.131.1.214-223.1977

Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12.

W Natter, D Sens, E James
PMCID: PMC235412  PMID: 326762

Abstract

Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of the level of messenger RNA (mRNA) transcribed from seven of the nine genes of the arginine regulon of Escherichia coli K-12. The quantity of RNA complexing with each of the separated DNA strands of the argA, argF, argE, and argCBH operons carried on specialized transducing phages was measured. The derepressed:repressed ratio of mRNA formed in vivo was found to vary between about 3 and 4 when measured by hybridization to DNA isolated from specialized transducing phages carrying the argA, argE, argCBH, argF, and argI operons.

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Selected References

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