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. 1977 Jul;131(1):251–258. doi: 10.1128/jb.131.1.251-258.1977

Characterization of a plasmid from Streptomyces coelicolor A3(2).

H Schrempf, W Goebel
PMCID: PMC235417  PMID: 873884

Abstract

Covalently closed circular deoxyribonucleic acid (DNA) with a molecular weight of 20 X 10(6) was identified in strains of Streptomyces coelicolor A3(2) of various fertility types. Hybridization studies and digestion by various restriction endonucleases indicated that the circular DNAs (pSH1) were identical regardless of the fertility type (UF, IF, or NF) of the strain from which it was isolated. The pSH1 DNA was cleaved to many fragments by the endonucleases HincII, SmaI, and SalI and to three or four fragments by BamHI and PstI. Plasmid pSH1 carries single sites for each of the two restriction enzymes, EcoRI and HindIII. These sites are 7.6 X 10(6) daltons apart. Attempts to isolate the fertility factor SCP1 as covalently closed circular DNA were unsuccessful. These data suggest that the biochemically isolated plasmid pSH1 is not identical to the genetically characterized fertility factor SCP1, which has been identified in an autonomous state in IF-type strains and in an integrated state in NF-type strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bujard H. Electron microscopy of single-stranded DNA. J Mol Biol. 1970 Apr 14;49(1):125–137. doi: 10.1016/0022-2836(70)90381-5. [DOI] [PubMed] [Google Scholar]
  2. DeFilippes F. M. A new method for isolation of a restriction enzyme from Hemophilus parainfluenzae. Biochem Biophys Res Commun. 1974 Jun 4;58(3):586–596. doi: 10.1016/s0006-291x(74)80460-2. [DOI] [PubMed] [Google Scholar]
  3. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  4. Goebel W., Schrempf H. Isolation of minicircular deoxyribonucleic acids from wild strains of Escherichia coli and their relationship to other bacterial plasmids. J Bacteriol. 1972 Sep;111(3):696–704. doi: 10.1128/jb.111.3.696-704.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hedgpeth J., Goodman H. M., Boyer H. W. DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448–3452. doi: 10.1073/pnas.69.11.3448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. Advances in Streptomyces coelicolor genetics. Bacteriol Rev. 1973 Sep;37(3):371–405. doi: 10.1128/br.37.3.371-405.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hopwood D. A. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967 Dec;31(4):373–403. doi: 10.1128/br.31.4.373-403.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hopwood D. A., Wright H. M. A plasmid of Streptomyces coelicolor carrying a chromosomal locus and its inter-specific transfer. J Gen Microbiol. 1973 Dec;79(2):331–342. doi: 10.1099/00221287-79-2-331. [DOI] [PubMed] [Google Scholar]
  10. Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
  11. Lomovskaya N. D., Mkrtumian N. M., Gostimskaya N. L., Danilenko V. N. Characterization of temperate actinophage phi C31 isolated from Streptomyces coelicolor A3(2). J Virol. 1972 Feb;9(2):258–262. doi: 10.1128/jvi.9.2.258-262.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schrempf H., Bujard H., Hopwood D. A., Goebel W. Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2). J Bacteriol. 1975 Feb;121(2):416–421. doi: 10.1128/jb.121.2.416-421.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith D. I., Blattner F. R., Davies J. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii. Nucleic Acids Res. 1976 Feb;3(2):343–353. doi: 10.1093/nar/3.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith H. O., Wilcox K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970 Jul 28;51(2):379–391. doi: 10.1016/0022-2836(70)90149-x. [DOI] [PubMed] [Google Scholar]
  15. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wilson G. A., Young F. E. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J Mol Biol. 1975 Sep 5;97(1):123–125. doi: 10.1016/s0022-2836(75)80028-3. [DOI] [PubMed] [Google Scholar]
  17. Wright L. F., Hopwood D. A. Identification of the antibiotic determined by the SCP1 plasmid of Streptomyces coelicolor A3(2). J Gen Microbiol. 1976 Jul;95(1):96–106. doi: 10.1099/00221287-95-1-96. [DOI] [PubMed] [Google Scholar]

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