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. 1977 Jul;131(1):347–355. doi: 10.1128/jb.131.1.347-355.1977

Identification of lipopolysaccharides and phospholipids of Escherichia coli in polyacrylamide gels.

S C Bailey, D Apirion
PMCID: PMC235428  PMID: 326766

Abstract

Polyacrylamide gel electrophoresis of unfractionated lysates of radioactively labeled cells resolves not only proteins and polynucleotides into discrete bands but also cellular lipopolysaccharides and phospholipids. This allows a determination of the intracellular amounts of all of these macromolecules. In addition, this technique is sensitive enough to detect mutational alterations in lipopolysaccharide structure. Polyacrylamide gel electrophoresis is herein shown to be a useful tool for investigations into the structure of lipopolysaccharides and the synthesis of lipopolysaccharides and phospholipids.

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Selected References

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