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. 1977 Aug;131(2):493–498. doi: 10.1128/jb.131.2.493-498.1977

Outer membrane phospholipase A from Acinetobacter sp. HO1-N.

R E Torregrossa, R A Makula, W R Finnerty
PMCID: PMC235456  PMID: 407212

Abstract

A phospholipase A1 activity that hydrolyzed cardiolipin to triacyl- and diacyl-lysocardiolipin was localized in outer membrane preparations derived from Acinetobacter sp. HO1-N. The specific activity of the enzyme derived from hexadecane-grown cells was 2.5 to 3 times higher than that derived from NBYE-grown cells. An apparent Km of 2.22 mM was determined, although inhibition kinetics resulted at the higher cardiolipin substrate concentrations. Optimal reaction conditions established on metal requirements. Enzyme activity was obligately dependent on Triton X-100 (0.5%) and was inhibited by cationic and anionic detergents. Cardiolipin-specific phospholipase D converted triacyl-lysocardiolipin to lysophosphatidylglycerol and phosphatidic acid. The specific activity of this enzyme was approximately 100 times greater than that reported for other membrane preparations derived from microorganisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albright F. R., White D. A., Lennarz W. J. Studies on enzymes involved in the catabolism of phospholipids in Escherichia coli. J Biol Chem. 1973 Jun 10;248(11):3968–3977. [PubMed] [Google Scholar]
  2. Astrachan L. The bond hydrolyzed by cardiolipin-specific phospholipase D. Biochim Biophys Acta. 1973 Jan 19;296(1):79–88. doi: 10.1016/0005-2760(73)90046-5. [DOI] [PubMed] [Google Scholar]
  3. Audet A., Cole R., Proulx P. Polyglycerophosphatide metabolism in Escherichia coli. Biochim Biophys Acta. 1975 Mar 24;380(3):414–420. doi: 10.1016/0005-2760(75)90109-5. [DOI] [PubMed] [Google Scholar]
  4. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  5. Doi O., Oki M., Nojima S. Two kinds of phospholipase A and lysophospholipase in Escherichia coli. Biochim Biophys Acta. 1972 Feb 21;260(2):244–258. [PubMed] [Google Scholar]
  6. Finnerty W. R., Makula R. A. Microbial lipid metabolism. CRC Crit Rev Microbiol. 1975 Oct;4(1):1–40. doi: 10.3109/10408417509105485. [DOI] [PubMed] [Google Scholar]
  7. Kent C., Lennarz W. J. An osmotically fragile mutant of Bacillus subtilis with an active membrane-associated phospholipase A 1 . Proc Natl Acad Sci U S A. 1972 Oct;69(10):2793–2797. doi: 10.1073/pnas.69.10.2793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LANDS W. E. LIPID METABOLISM. Annu Rev Biochem. 1965;34:313–346. doi: 10.1146/annurev.bi.34.070165.001525. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Makula R. A., Finnerty W. R. Microbial assimilation of hydrocarbons: cellular distribution of fatty acids. J Bacteriol. 1972 Oct;112(1):398–407. doi: 10.1128/jb.112.1.398-407.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Makula R. A., Finnerty W. R. Microbial assimilation of hydrocarbons: phospholipid metabolism. J Bacteriol. 1971 Sep;107(3):806–814. doi: 10.1128/jb.107.3.806-814.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nishijima M., Akamatsu Y., Nojima S. Purification and properties of a membrane-bound phospholipase A1 from Mycobacterium phlei. J Biol Chem. 1974 Sep 10;249(17):5658–5667. [PubMed] [Google Scholar]
  13. Ono Y., Nojima S. Phospholipases of the membrane fraction of Mycobacterium phlei. Biochim Biophys Acta. 1969 Jan 21;176(1):111–119. [PubMed] [Google Scholar]
  14. Powell G. L., Jacobus J. The nonequivalence of the phosphorus atoms in cardiolipin. Biochemistry. 1974 Sep 10;13(19):4024–4026. doi: 10.1021/bi00716a032. [DOI] [PubMed] [Google Scholar]
  15. Raybin D. M., Bertsch L. L., Kornberg A. A phospholipase in Bacillus megaterium unique to spores and sporangia. Biochemistry. 1972 May 9;11(10):1754–1760. doi: 10.1021/bi00760a004. [DOI] [PubMed] [Google Scholar]
  16. Sampson K. L., Finnerty W. R. Regulation of fatty acid biosynthesis in the hydrocarbon oxidizing microorganism, Acinetobacter sp. Arch Microbiol. 1974;99(3):203–220. doi: 10.1007/BF00696235. [DOI] [PubMed] [Google Scholar]
  17. Scandella C. J., Kornberg A. A membrane-bound phospholipase A1 purified from Escherichia coli. Biochemistry. 1971 Nov 23;10(24):4447–4456. doi: 10.1021/bi00800a015. [DOI] [PubMed] [Google Scholar]
  18. Scott C. C., Makula S. R., Finnerty W. R. Isolation and characterization of membranes from a hydrocarbon-oxidizing Acinetobacter sp. J Bacteriol. 1976 Jul;127(1):469–480. doi: 10.1128/jb.127.1.469-480.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Senff L. M., Wegener W. S., Brooks G. F., Finnerty W. R., Makula R. A. Phospholipid composition and phospholipase A activity of Neisseria gonorrhoeae. J Bacteriol. 1976 Aug;127(2):874–880. doi: 10.1128/jb.127.2.874-880.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Short S. A., White D. C. Metabolism of the glycosyl diglycerides and phosphatidylglucose of Staphylococcus aureus. J Bacteriol. 1970 Oct;104(1):126–132. doi: 10.1128/jb.104.1.126-132.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Torregrossa R. E., Makula R. A., Finnerty W. R. Characterization of lysocardiolipin from Acinetobacter sp. HO1-N. J Bacteriol. 1977 Aug;131(2):486–492. doi: 10.1128/jb.131.2.486-492.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tucker A. N., White D. C. Detection of a rapidly metabolizing portion of the membrane cardiolipin in Haemophilus parainfluenzae. J Bacteriol. 1971 Dec;108(3):1058–1064. doi: 10.1128/jb.108.3.1058-1064.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. de Haas G. H., Sarda L., Roger J. Positional specific hydrolysis of phospholipids by pancreatic lipase. Biochim Biophys Acta. 1965 Dec 2;106(3):638–640. doi: 10.1016/0005-2760(65)90082-2. [DOI] [PubMed] [Google Scholar]
  24. van Deenen L. L. Some structural and dynamic aspects of lipids in biological membranes. Ann N Y Acad Sci. 1966 Jul 14;137(2):717–730. doi: 10.1111/j.1749-6632.1966.tb50193.x. [DOI] [PubMed] [Google Scholar]

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