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. 1977 Aug;131(2):557–563. doi: 10.1128/jb.131.2.557-563.1977

Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae.

M Roberts, L P Elwell, S Falkow
PMCID: PMC235464  PMID: 407214

Abstract

The molecular nature of two distinct gonococcal R plasmids, 4.4 X 10(6) and 3.2 X 10(6) daltons, encoding beta-lactamase activity were examined. Both plasmids contained about 40% of the transposable ampicillin resistance sequence Tn2. Deoxyribonucleic acid-deoxyribonucleic acid polynucleotide sequence studies have shown that the two gonococcal plasmids share about 70% of their sequences and are closely related to RSF0885, a 4.1 X 10(6)-dalton plasmid found in a beta-lactamase-producing strain of Haemophilus influenzae. All three of these R plasmids possess a guanine-plus-cytosine content of 0.40 to 0.41 mol fraction and are present as multicopy gene pools in their bacterial hosts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Lewis M. J. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature. 1965 Nov 27;208(5013):843–849. doi: 10.1038/208843a0. [DOI] [PubMed] [Google Scholar]
  2. Ashford W. A., Golash R. G., Hemming V. G. Penicillinase-producing Neisseria gonorrhoeae. Lancet. 1976 Sep 25;2(7987):657–658. doi: 10.1016/s0140-6736(76)92467-3. [DOI] [PubMed] [Google Scholar]
  3. Bennett P. M., Richmond M. H. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli. J Bacteriol. 1976 Apr;126(1):1–6. doi: 10.1128/jb.126.1.1-6.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  5. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen S. N., Kopecko D. J. Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA sequence insertions. Fed Proc. 1976 Jul;35(9):2031–2036. [PubMed] [Google Scholar]
  7. Cohen S. N. Transposable genetic elements and plasmid evolution. Nature. 1976 Oct 28;263(5580):731–738. doi: 10.1038/263731a0. [DOI] [PubMed] [Google Scholar]
  8. Crosa J. H., Olarte J., Mata L. J., Luttropp L. K., Peñaranda M. E. Characterization of an R-plasmid associated with ampicillin resistance in Shigella dysenteriae type 1 isolated from epidemics. Antimicrob Agents Chemother. 1977 Mar;11(3):553–558. doi: 10.1128/aac.11.3.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. De Graaff J., Elwell L. P., Falkow S. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b. J Bacteriol. 1976 Apr;126(1):439–446. doi: 10.1128/jb.126.1.439-446.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Elwell L. P., Roberts M., Mayer L. W., Falkow S. Plasmid-mediated beta-lactamase production in Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1977 Mar;11(3):528–533. doi: 10.1128/aac.11.3.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Engelkirk P. G., Schoenhard D. E. Physical evidence of a plasmid in Neisseria gonorrhoeae. J Infect Dis. 1973 Feb;127(2):197–200. doi: 10.1093/infdis/127.2.197. [DOI] [PubMed] [Google Scholar]
  13. Fitzgerald T. J., Miller J. N., Sykes J. A. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. Infect Immun. 1975 May;11(5):1141–1146. doi: 10.1128/iai.11.5.1141-1146.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Heffron F., Rubens C., Falkow S. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3623–3627. doi: 10.1073/pnas.72.9.3623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Heffron F., Sublett R., Hedges R. W., Jacob A., Falkow S. Origin of the TEM-beta-lactamase gene found on plasmids. J Bacteriol. 1975 Apr;122(1):250–256. doi: 10.1128/jb.122.1.250-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kammer R. B., Preston D. A., Turner J. R., Hawley L. C. Rapid detection of ampicillin-resistant Haemophilus influenzae and their susceptibility to sixteen antibiotics. Antimicrob Agents Chemother. 1975 Jul;8(1):91–94. doi: 10.1128/aac.8.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mayer L. W., Holmes K. K., Falkow S. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae. Infect Immun. 1974 Oct;10(4):712–717. doi: 10.1128/iai.10.4.712-717.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Percival A., Rowlands J., Corkill J. E., Alergant C. D., Arya O. P., Rees E., Annels E. H. Penicillinase-producing Gonococci in Liverpool. Lancet. 1976 Dec 25;2(8000):1379–1382. doi: 10.1016/s0140-6736(76)91919-x. [DOI] [PubMed] [Google Scholar]
  22. Phillips I. Beta-lactamase-producing, penicillin-resistant gonococcus. Lancet. 1976 Sep 25;2(7987):656–657. doi: 10.1016/s0140-6736(76)92466-1. [DOI] [PubMed] [Google Scholar]
  23. Roberts M., Falkow S. Conjugal transfer of R plasmids in Neisseria gonorrhoeae. Nature. 1977 Apr 14;266(5603):630–631. doi: 10.1038/266630a0. [DOI] [PubMed] [Google Scholar]
  24. So M., Crosa J. H., Falkow S. Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids. J Bacteriol. 1975 Jan;121(1):234–238. doi: 10.1128/jb.121.1.234-238.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stiffler P. W., Lerner S. A., Bohnhoff M., Morello J. A. Plasmid deoxyribonucleic acid in clinical isolates of Neisseria gonorrhoeae. J Bacteriol. 1975 Jun;122(3):1293–1300. doi: 10.1128/jb.122.3.1293-1300.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Van A. D., Bieth G., Bouanchaud D. H. Résistance plasmidique à la tétracycline chez Haemophilus influenzae. C R Acad Sci Hebd Seances Acad Sci D. 1975 Mar 10;280(10):1321–1323. [PubMed] [Google Scholar]
  27. Van A. D., Goldstein F., Acar J. F., Bouanchaud D. H. A transferable kanamycin resistance plasmid isolated from Haemophilus influenzae. Ann Microbiol (Paris) 1975 Apr;126(3):397–399. [PubMed] [Google Scholar]
  28. van Klingeren B., van Embden J. D., Dessens-Kroon M. Plasmid-mediated chloramphenicol resistance in Haemophilus influenzae. Antimicrob Agents Chemother. 1977 Mar;11(3):383–387. doi: 10.1128/aac.11.3.383. [DOI] [PMC free article] [PubMed] [Google Scholar]

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