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. 1977 Aug;131(2):583–588. doi: 10.1128/jb.131.2.583-588.1977

Characterization of an endonuclease associated with the drug resistance plasmid pKM101.

D Lackey, G C Walker, T Keng, S Linn
PMCID: PMC235467  PMID: 18440

Abstract

An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a molecular weight of roughly 75,000 and, at pH 7.0, was equally active on single-stranded and duplex deoxyribonucleic acid (DNA). The reaction with single-stranded DNA was optimal at pH 5.5, whereas with duplex DNA the optimum was pH 6.8. The enzyme required a divalent cation for activity, and it had no detectable exonuclease activity with single-stranded or duplex DNA. The endonuclease extensively degraded DNA with no apparent base specificity, forming 5'-phosphomonoester termini. Although characterization of the endonuclease has not revealed its function, the enzyme does not appear to be a restriction endonuclease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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