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. 1977 Aug;131(2):713–715. doi: 10.1128/jb.131.2.713-715.1977

Spore coat protein and enterotoxin synthesis in Clostridium perfringens.

R G Labbe, C L Duncan
PMCID: PMC235488  PMID: 195933

Abstract

Polyacrylamide gel profiles of Clostridium perfringens spore coat protein revealed four and occasionally five components. Pulse-chase experiments indicated that synthesis of coat protein polypeptide and enterotoxin was an early sporulation event. However, maximum synthesis occurred coincident with the onset of heat resistance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson A. I., Fitz-James P. C. Biosynthesis of bacterial spore coats. J Mol Biol. 1968 Apr 14;33(1):199–212. doi: 10.1016/0022-2836(68)90288-x. [DOI] [PubMed] [Google Scholar]
  2. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  3. Frieben W. R., Duncan C. L. Homology between enterotoxin protein and spore structural protein in Clostridium perfringens type A. Eur J Biochem. 1973 Nov 15;39(2):393–401. doi: 10.1111/j.1432-1033.1973.tb03137.x. [DOI] [PubMed] [Google Scholar]
  4. Kondo M., Foster J. W. Chemical and electron microscope studies on fractions prepared from coats of Bacillus spores. J Gen Microbiol. 1967 May;47(2):257–271. doi: 10.1099/00221287-47-2-257. [DOI] [PubMed] [Google Scholar]
  5. Labbe R. G., Duncan C. L. Evidence for stable messenger ribonucleic acid during sporulation and enterotoxin synthesis by Clostridium perfringens type A. J Bacteriol. 1977 Feb;129(2):843–849. doi: 10.1128/jb.129.2.843-849.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Labbe R. G., Duncan C. L. Growth from spores of Clostridium perfringens in the presence of sodium nitrite. Appl Microbiol. 1970 Feb;19(2):353–359. doi: 10.1128/am.19.2.353-359.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Labbe R. G., Duncan C. L. Sporulation and enterotoxin production by Clostridium perfringens type A under conditions of controlled pH and temperature. Can J Microbiol. 1974 Nov;20(11):1493–1501. doi: 10.1139/m74-233. [DOI] [PubMed] [Google Scholar]
  8. Labbe R. G., Duncan C. L. Synthesis of deoxyribonucleic acid, ribonucleic acid, and protein during sporulation of Clostridium perfringens. J Bacteriol. 1976 Feb;125(2):444–452. doi: 10.1128/jb.125.2.444-452.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lecadet M. M., Chevrier G., Dedonder R. Analysis of a protein fraction in the spore coats of Bacillus thuringiensis. Comparison with crystal protein. Eur J Biochem. 1972 Feb 15;25(2):349–358. doi: 10.1111/j.1432-1033.1972.tb01703.x. [DOI] [PubMed] [Google Scholar]
  10. Somerville H. J., Delafield F. P., Rittenberg S. C. Urea-mercaptoethanol-soluble protein from spores of Bacillus thuringiensis and other species. J Bacteriol. 1970 Feb;101(2):551–560. doi: 10.1128/jb.101.2.551-560.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spudich J. A., Kornberg A. Biochemical studies of bacterial sporulation and germination. VI. Origin of spore core and coat proteins. J Biol Chem. 1968 Sep 10;243(17):4588–4599. [PubMed] [Google Scholar]
  12. Stark R. L., Duncan C. L. Biological characteristics of Clostridium perfringens type A enterotoxin. Infect Immun. 1971 Aug;4(2):89–96. doi: 10.1128/iai.4.2.89-96.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stark R. L., Duncan C. L. Purification and biochemical properties of Clostridium perfringens type A enterotoxin. Infect Immun. 1972 Nov;6(5):662–673. doi: 10.1128/iai.6.5.662-673.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vary J. C. Germination of Bacillus megaterium spores after various extraction procedures. J Bacteriol. 1973 Nov;116(2):797–802. doi: 10.1128/jb.116.2.797-802.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wood D. A. Sporulation in Bacillus subtilis. Properties and time of synthesis of alkali-soluble protein of the spore coat. Biochem J. 1972 Nov;130(2):505–514. doi: 10.1042/bj1300505. [DOI] [PMC free article] [PubMed] [Google Scholar]

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