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. 1977 Sep;131(3):735–740. doi: 10.1128/jb.131.3.735-740.1977

Killer double-stranded ribonucleic acid synthesis in cell division cycle mutants of Saccharomyces cerevisiae.

C Shalitin, I Weiser
PMCID: PMC235523  PMID: 330495

Abstract

The synthesis of killer double-stranded ribonucleic acid (dsRNA) in Saccharomyces cerevisiae was examined in seven different cell division cycle mutants (cdc) that are defective in nuclear deoxyribonucleic acid replication and contain the "killer character." In cdc28, cdc4, and cdc7, which are defective in the initiation of nuclear deoxyribonucleic acid synthesis, and in cdc23 or in cdc14, defective in medial or late nuclear division, an overproduction of dsRNA at the restrictive temperature was observed. In contrast to the above mutants, the synthesis of killer dsRNA is not enhanced at the restrictive temperature in either cdc8 or cdc21, which are defective in deoxyribonucleic acid chain elongation. Examination of killer sensitive strains (cdc7 K- and cdc4 K-) has shown that the complete killer dsRNA genome is essential for the overproduction of dsRNA at the restrictive temperature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARLOW J. J., MATHIAS A. P., WILLIAMSON R., GAMMACK D. B. A SIMPLE METHOD FOR THE QUANTITATIVE ISOLATION OF UNDEGRADED HIGH MOLECULAR WEIGHT RIBONUCLEIC ACID. Biochem Biophys Res Commun. 1963 Sep 10;13:61–66. doi: 10.1016/0006-291x(63)90163-3. [DOI] [PubMed] [Google Scholar]
  2. Bussey H., Sherman D., Somers J. M. Action of yeast killer factor: a resistant mutant with sensitive spheroplasts. J Bacteriol. 1973 Mar;113(3):1193–1197. doi: 10.1128/jb.113.3.1193-1197.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cottrell S., Rabinowitz M., Getz G. S. Mitochondrial deoxyribonucleic acid synthesis in a temperature-sensitive mutant of deoxyribonucleic acid replication of Saccharomyces cerevisiae. Biochemistry. 1973 Oct 23;12(22):4374–4378. doi: 10.1021/bi00746a012. [DOI] [PubMed] [Google Scholar]
  4. Cryer D. R., Goldthwaite C. D., Zinker S., Lam K. B., Storm E., Hirschberg R., Blamire J., Finkelstein D. B., Marmur J. Studies on nuclear and mitochondrial DNA of Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol. 1974;38:17–29. doi: 10.1101/sqb.1974.038.01.005. [DOI] [PubMed] [Google Scholar]
  5. Fink G. R., Styles C. A. Curing of a killer factor in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2846–2849. doi: 10.1073/pnas.69.10.2846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fischer I., Shalitin C. Increased synthesis of abundant poly(A)-containing RNA in a DNA defective mutant of Saccharomyces cerevisiae containing the "killer character". Biochim Biophys Acta. 1977 Mar 2;475(1):64–73. doi: 10.1016/0005-2787(77)90339-2. [DOI] [PubMed] [Google Scholar]
  7. Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Game J. C. Yeast cell-cycle mutant cdc21 is a temperature-sensitive thymidylate auxotroph. Mol Gen Genet. 1976 Aug 2;146(3):313–315. doi: 10.1007/BF00701257. [DOI] [PubMed] [Google Scholar]
  9. Hartwell L. H. Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation. J Mol Biol. 1971 Jul 14;59(1):183–194. doi: 10.1016/0022-2836(71)90420-7. [DOI] [PubMed] [Google Scholar]
  10. Hartwell L. H., Mortimer R. K., Culotti J., Culotti M. Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics. 1973 Jun;74(2):267–286. doi: 10.1093/genetics/74.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hartwell L. H. Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures. J Bacteriol. 1970 Dec;104(3):1280–1285. doi: 10.1128/jb.104.3.1280-1285.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hartwell L. H. Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. J Bacteriol. 1973 Sep;115(3):966–974. doi: 10.1128/jb.115.3.966-974.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hatzfeld J. DNA labelling and its assay in yeast. Biochim Biophys Acta. 1973 Feb 23;299(1):34–42. doi: 10.1016/0005-2787(73)90395-x. [DOI] [PubMed] [Google Scholar]
  14. Newlon C. S., Fangman W. L. Mitochondrial DNA synthesis in cell cycle mutants of Saccharomyces cerevisiae. Cell. 1975 Aug;5(4):423–428. doi: 10.1016/0092-8674(75)90061-6. [DOI] [PubMed] [Google Scholar]
  15. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  16. Petes T. D., Williamson D. H. Replicating circular DNA molecules in yeast. Cell. 1975 Mar;4(3):249–253. doi: 10.1016/0092-8674(75)90172-5. [DOI] [PubMed] [Google Scholar]
  17. Shalitin C., Fischer I. Abundant species of poly(A)-containing RNA from Saccharomyces cerevisiae. Biochim Biophys Acta. 1975 Dec 19;414(3):263–272. doi: 10.1016/0005-2787(75)90165-3. [DOI] [PubMed] [Google Scholar]
  18. Shatkin A. J., Sipe J. D., Loh P. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J Virol. 1968 Oct;2(10):986–991. doi: 10.1128/jvi.2.10.986-991.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vodkin M. Homology between double-stranded RNA and nuclear DNA of yeast. J Virol. 1977 Feb;21(2):516–521. doi: 10.1128/jvi.21.2.516-521.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vodkin M., Katterman F., Fink G. R. Yeast killer mutants with altered double-stranded ribonucleic acid. J Bacteriol. 1974 Feb;117(2):681–686. doi: 10.1128/jb.117.2.681-686.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wickner R. B. Killer of Saccharomyces cerevisiae: a double-stranded ribonucleic acid plasmid. Bacteriol Rev. 1976 Sep;40(3):757–773. doi: 10.1128/br.40.3.757-773.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wintersberger U., Hirsch J., Fink A. M. Studies on nuclear and mitochondrial DNA-replication in a temperature-sensitive mutant of Saccharomyces cerevisiae. Mol Gen Genet. 1974;131(4):291–299. doi: 10.1007/BF00264860. [DOI] [PubMed] [Google Scholar]
  23. al-Aidroos K., Somers J. M., Bussey H. Retention of cytoplasmic killer determinants in yeast cells after removal of mitochondrial DNA by ethidium bromide. Mol Gen Genet. 1973 May 28;122(4):323–330. doi: 10.1007/BF00269432. [DOI] [PubMed] [Google Scholar]

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