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. 1977 Sep;131(3):741–744. doi: 10.1128/jb.131.3.741-744.1977

Zeatin ribonucleosides in the transfer ribonucleic acid of Rhizobium leguminosarum, Agrobacterium tumefaciens, Corynebacterium fascians, and Erwinia amylovora.

J D Cherayil, M N Lipsett
PMCID: PMC235524  PMID: 893341

Abstract

Until recently, the presence in transfer ribonucleic acid (tRNA) of the hydroxylated cytokinin ribosylzeatin [N6-(4-hydroxy-3-methylbut-2-enyl)adenosine]was thought to be unique to higher plants. This extension of work from several laboratories indicates the presence of 2-methylthioribosylzeatin in the tRNA of the plant-associated bacteria Rhizobium leguminosarum, Agrobacterium tumefaciens, and Corynebacterium fascians, but not in that of Erwinia amylovora. This cytokinin has the cis configuration, as is normally found in the tRNA's of plants. The tRNA thionucleotide patterns in these bacteria are different from those of Escherichia coli, Bacillus subtilis, and Salmonella typhimurium, which contain the unhydroxylated analogs of ribosylzeatin or 2-methylthioribosylzeatin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUNNGRABER E. F. A simplified procedure for the preparation of "soluble" RNA from rat liver. Biochem Biophys Res Commun. 1962 Jun 19;8:1–3. doi: 10.1016/0006-291x(62)90223-1. [DOI] [PubMed] [Google Scholar]
  2. Burrows W. J., Armstrong D. J., Kaminek M., Skoog F., Bock R. M., Hecht S. M., Dammann L. G., Leonard N. J., Occolowitz J. Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid. Biochemistry. 1970 Apr 28;9(9):1867–1872. doi: 10.1021/bi00811a001. [DOI] [PubMed] [Google Scholar]
  3. Burrows W. J., Armstrong D. J., Skoog F., Hecht S. M., Boyle J. T., Leonard N. J., Occolowitz J. Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine. Science. 1968 Aug 16;161(3842):691–693. doi: 10.1126/science.161.3842.691. [DOI] [PubMed] [Google Scholar]
  4. Chapman R. W., Morris R. O., Zaerr J. B. Occurrence of trans-ribosylzeatin in Agrobacterium tumefaciens tRNA. Nature. 1976 Jul 8;262(5564):153–154. doi: 10.1038/262153a0. [DOI] [PubMed] [Google Scholar]
  5. Hall R. H., Csonka L., David H., McLennan B. Cytokinins in the soluble RNA of plant tissues. Science. 1967 Apr 7;156(3771):69–71. doi: 10.1126/science.156.3771.69. [DOI] [PubMed] [Google Scholar]
  6. Hall R. H. N6-(delta 2-isopentenyl)adenosine: chemical reactions, biosynthesis, metabolism, and significance to the structure and function of tRNA. Prog Nucleic Acid Res Mol Biol. 1970;10:57–86. doi: 10.1016/s0079-6603(08)60561-9. [DOI] [PubMed] [Google Scholar]
  7. Klämbt D., Thies G., Skoog F. Isolation of cytokinins from Corynebacterium fascians. Proc Natl Acad Sci U S A. 1966 Jul;56(1):52–59. doi: 10.1073/pnas.56.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lipsett M. N. The isolation of 4-thiouridylic acid from the soluble ribonucleic acid of Escherichia coli. J Biol Chem. 1965 Oct;240(10):3975–3978. [PubMed] [Google Scholar]
  9. Phillips D. A., Torrey J. G. Studies on cytokinin production by Rhizobium. Plant Physiol. 1972 Jan;49(1):11–15. doi: 10.1104/pp.49.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Playtis A. J., Leonard N. J. The synthesis of ribosyl-cis-zeatin and thin layer chromatographic separation of the cis and trans isomers of ribosylzeatin. Biochem Biophys Res Commun. 1971 Oct 1;45(1):1–5. doi: 10.1016/0006-291x(71)90041-6. [DOI] [PubMed] [Google Scholar]
  11. Pugashetti B. K., Starr M. P. Conjugational transfer of genes determining plant virulence in Erwinia amylovora. J Bacteriol. 1975 May;122(2):485–491. doi: 10.1128/jb.122.2.485-491.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rao Y. S., Cherayil J. D. Thionucleoside composition of transfer RNAs in Bacillus subtilis and Salmonella typhimurium. Indian J Biochem Biophys. 1975 Jun;12(2):183–184. [PubMed] [Google Scholar]
  13. Thimmappaya B., Cherayil J. D. Unique presence of 2-methylthio-ribosylzeatin in the transfer ribonucleic acid of the bacterium Pseudomonas aeruginosa. Biochem Biophys Res Commun. 1974 Sep 23;60(2):665–672. doi: 10.1016/0006-291x(74)90292-7. [DOI] [PubMed] [Google Scholar]
  14. Vreman H. J., Skoog F. Cytokinins in pisum transfer ribonucleic Acid. Plant Physiol. 1972 May;49(5):848–851. doi: 10.1104/pp.49.5.848. [DOI] [PMC free article] [PubMed] [Google Scholar]

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