Abstract
Flagellar mutants in Escherichia coli region I were obtained by selection for resistance to the flagellotropic phage chi. F' elements carrying this region of the E. coli genome were then constructed. Stable merodiploid strains with a flagellar defect on the exogenate and another on the endogenote were prepared. These merodiploids yielded information on the complementation behavior of mutations in this region. Region I was shown to include at least six cistrons, flaV, flaK, flaL, flaM, flaS, and flaT. Mu-induced and deletion fla mutants were also isolated. By using these mutant strains, the transcriptional order was shown to be flaV-flaK-flaL-flaM-flaS-flaT. The definition of region I fla genes and their transcriptional relationships were confirmed by genetic tests with hybrid A phage carrying fla genes in this region.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Armstrong J. B., Adler J. Genetics of motility in Escherichia coli: complementation of paralysed mutants. Genetics. 1967 Jul;56(3):363–373. doi: 10.1093/genetics/56.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DePamphilis M. L., Adler J. Purification of intact flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):376–383. doi: 10.1128/jb.105.1.376-383.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hilmen M., Silverman M., Simon M. The regulation of flagellar formation and function. J Supramol Struct. 1974;2(2-4):360–371. doi: 10.1002/jss.400020225. [DOI] [PubMed] [Google Scholar]
- Komeda Y., Icho T., Iino T. Effects of galU mutation on flagellar formation in Escherichia coli. J Bacteriol. 1977 Feb;129(2):908–915. doi: 10.1128/jb.129.2.908-915.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komeda Y., Shimada K., Iino T. Isolation of specialized lambda transducing bacteriophages for flagellar genes (fla) of Escherichia coli K-12. J Virol. 1977 Jun;22(3):654–661. doi: 10.1128/jvi.22.3.654-661.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komeda Y., Suzuki H., Ishidsu J. I., Iino T. The role of cAMP in flagellation of Salmonella typhimurium. Mol Gen Genet. 1976 Dec 31;142(4):289–298. doi: 10.1007/BF00271253. [DOI] [PubMed] [Google Scholar]
- Kondoh H., Ozeki H. Deletion and amber mutants of fla loci in Escherichia coli K-12. Genetics. 1976 Nov;84(3):403–421. doi: 10.1093/genetics/84.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MEYNELL E. W. A phage, phi chi, which attacks motile bacteria. J Gen Microbiol. 1961 Jun;25:253–290. doi: 10.1099/00221287-25-2-253. [DOI] [PubMed] [Google Scholar]
- Matsumura P., Silverman M., Simon M. Cloning and expression of the flagellar hook gene on hybird plasmids in minicells. Nature. 1977 Feb 24;265(5596):758–760. doi: 10.1038/265758a0. [DOI] [PubMed] [Google Scholar]
- Parkinson J. S., Huskey R. J. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J Mol Biol. 1971 Mar 14;56(2):369–384. doi: 10.1016/0022-2836(71)90471-2. [DOI] [PubMed] [Google Scholar]
- Silverman M., Matsumura P., Simon M. The identification of the mot gene product with Escherichia coli-lambda hybrids. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3126–3130. doi: 10.1073/pnas.73.9.3126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silverman M., Simon M. Genetic analysis of bacteriophage Mu-induced flagellar mutants in Escherichia coli. J Bacteriol. 1973 Oct;116(1):114–122. doi: 10.1128/jb.116.1.114-122.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silverman M., Simon M. Genetic analysis of flagellar mutants in Escherichia coli. J Bacteriol. 1973 Jan;113(1):105–113. doi: 10.1128/jb.113.1.105-113.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silverman M., Simon M. Positioning flagellar genes in Escherichia coli by deletion analysis. J Bacteriol. 1974 Jan;117(1):73–79. doi: 10.1128/jb.117.1.73-79.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vary P. S., Stocker B. A. Nonsense motility mutants in Salmonella typhimurium. Genetics. 1973 Feb;73(2):229–245. doi: 10.1093/genetics/73.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamaguchi S., Iino T., Horiguchi T., Ota K. Genetic analysis of fla and mot cistrons closely linked to H1 in Salmonella abortusequi and its derivatives. J Gen Microbiol. 1972 Apr;70(1):59–75. doi: 10.1099/00221287-70-1-59. [DOI] [PubMed] [Google Scholar]