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. 1977 Sep;131(3):801–808. doi: 10.1128/jb.131.3.801-808.1977

Genetic analysis of Escherichia coli K-12 region I flagellar mutants.

Y Komeda, M Silverman, M Simon
PMCID: PMC235534  PMID: 330498

Abstract

Flagellar mutants in Escherichia coli region I were obtained by selection for resistance to the flagellotropic phage chi. F' elements carrying this region of the E. coli genome were then constructed. Stable merodiploid strains with a flagellar defect on the exogenate and another on the endogenote were prepared. These merodiploids yielded information on the complementation behavior of mutations in this region. Region I was shown to include at least six cistrons, flaV, flaK, flaL, flaM, flaS, and flaT. Mu-induced and deletion fla mutants were also isolated. By using these mutant strains, the transcriptional order was shown to be flaV-flaK-flaL-flaM-flaS-flaT. The definition of region I fla genes and their transcriptional relationships were confirmed by genetic tests with hybrid A phage carrying fla genes in this region.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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