Abstract
The kinetic features of the myo-inositol transport system in Pseudomonas putida are reported. The system is sensitive to osmotic shock, is not operative in membrane vesicles, and does not involved substrate phosphorylation. Line-weaver-Burk plots indicate the presence of two different systems, whose Kt are 5 micrometer and 0.43 mM and whose V max are 7.9 and 27 nml/mg per min, respectively. Transport activity of glucose-grown cells is very low. myo-Inositol-grown cells lose the high-affinity system upon osmotic shock; concentrated shock fluid possesses myo-inositol-binding activity. The system is very specific for the myo-configuration of the cyclitol.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. I. LA BIOSYNTH'ESE INDUITE DE LA GALACTOKINASE ET L'INDUCTION SIMULTAN'EE DE LA S'EQUENCE ENZYMATIQUE. J Mol Biol. 1963 Aug;7:164–182. doi: 10.1016/s0022-2836(63)80044-3. [DOI] [PubMed] [Google Scholar]
- Berman T., Magasanik B. The pathway of myo-inositol degradation in Aerobacter aerogenes. Dehydrogenation and dehydration. J Biol Chem. 1966 Feb 25;241(4):800–806. [PubMed] [Google Scholar]
- Cheneval J. P., Deshusses J., Posternak T. Sur le transport de l'inositol chez Schizosaccharomyces pombe. Biochim Biophys Acta. 1970 Apr 21;203(2):348–350. doi: 10.1016/0005-2736(70)90152-5. [DOI] [PubMed] [Google Scholar]
- Cirillo V. P. Relationship between sugar structure and competition for the sugar transport system in Bakers' yeast. J Bacteriol. 1968 Feb;95(2):603–611. doi: 10.1128/jb.95.2.603-611.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deshusses J., Reber G. Assymetry of the myo-inositol transport system in Klebsiella aerogenes. Energy is necessary to create the assymetry of the transport system. Eur J Biochem. 1977 Jan 3;72(1):101–106. doi: 10.1111/j.1432-1033.1977.tb11229.x. [DOI] [PubMed] [Google Scholar]
- Deshusses J., Reber G. Myo-inositol transport in Aerobacter aerogenes. Biochim Biophys Acta. 1972 Aug 9;274(2):598–605. doi: 10.1016/0005-2736(72)90206-4. [DOI] [PubMed] [Google Scholar]
- Deshusses J., Reber G. Stereospecificity of the myo-inositol transport system of Aerobacter aerogenes and sensitivity towards sulfhydryl reagents. Experientia. 1974 Sep 15;30(9):996–997. doi: 10.1007/BF01938967. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
- Reber G., Mermod M., Deshusses J. Transport of cyclitols by a proton symport in Klebsiella aerogenes. Eur J Biochem. 1977 Jan 3;72(1):93–99. doi: 10.1111/j.1432-1033.1977.tb11228.x. [DOI] [PubMed] [Google Scholar]