Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Sep;131(3):1026–1028. doi: 10.1128/jb.131.3.1026-1028.1977

Kinase replacement by a dehydrogenase for Escherichia coli glycerol utilization.

E J St Martin, W B Freedberg, E C Lin
PMCID: PMC235566  PMID: 197059

Abstract

A mutant of Escherichia coli that employs a glycerol:nicotinamide adenine dinucleotide 2-oxidoreductase (EC 1.1.1.6), instead of adenosine 5'-triphosphate:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol was constructed. This mutant, like the wild-type strain, still cannot grow anaerobically on glycerol without an exogenous hydrogen acceptor.

Full text

PDF
1026

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASNIS R. E., BRODIE A. F. A glycerol dehydrogenase from Escherichia coli. J Biol Chem. 1953 Jul;203(1):153–159. [PubMed] [Google Scholar]
  2. Cozzarelli N. R., Koch J. P., Hayashi S., Lin E. C. Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli. J Bacteriol. 1965 Nov;90(5):1325–1329. doi: 10.1128/jb.90.5.1325-1329.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cozzarelli N. R., Lin E. C. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J Bacteriol. 1966 May;91(5):1763–1766. doi: 10.1128/jb.91.5.1763-1766.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Freedberg W. B., Lin E. C. Three kinds of controls affecting the expression of the glp regulon in Escherichia coli. J Bacteriol. 1973 Sep;115(3):816–823. doi: 10.1128/jb.115.3.816-823.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hayashi S. I., Lin E. C. Purification and properties of glycerol kinase from Escherichia coli. J Biol Chem. 1967 Mar 10;242(5):1030–1035. [PubMed] [Google Scholar]
  6. LIN E. C., LEVIN A. P., MAGASANIK B. The effect of aerobic metabolism on the inducible glycerol dehydrogenase of Aerobacter aerogenes. J Biol Chem. 1960 Jun;235:1824–1829. [PubMed] [Google Scholar]
  7. Lin E. C. Glycerol dissimilation and its regulation in bacteria. Annu Rev Microbiol. 1976;30:535–578. doi: 10.1146/annurev.mi.30.100176.002535. [DOI] [PubMed] [Google Scholar]
  8. Miki K., Lin E. C. Anaerobic energy-yielding reaction associated with transhydrogenation from glycerol 3-phosphate to fumarate by an Escherichia coli system. J Bacteriol. 1975 Dec;124(3):1282–1287. doi: 10.1128/jb.124.3.1282-1287.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miki K., Lin E. C. Electron transport chain from glycerol 3-phosphate to nitrate in Escherichia coli. J Bacteriol. 1975 Dec;124(3):1288–1294. doi: 10.1128/jb.124.3.1288-1294.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Richey D. P., Lin E. C. Importance of facilitated diffusion for effective utilization of glycerol by Escherichia coli. J Bacteriol. 1972 Nov;112(2):784–790. doi: 10.1128/jb.112.2.784-790.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ruch F. E., Lengeler J., Lin E. C. Regulation of glycerol catabolism in Klebsiella aerogenes. J Bacteriol. 1974 Jul;119(1):50–56. doi: 10.1128/jb.119.1.50-56.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ruch F. E., Lin E. C. Independent constitutive expression of the aerobic and anaerobic pathways of glycerol catabolism in Klebsiella aerogenes. J Bacteriol. 1975 Oct;124(1):348–352. doi: 10.1128/jb.124.1.348-352.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schwartz M. Location of the maltose A and B loci on the genetic map of Escherichia coli. J Bacteriol. 1966 Oct;92(4):1083–1089. doi: 10.1128/jb.92.4.1083-1089.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sridhara S., Wu T. T., Chused T. M., Lin E. C. Ferrous-activated nicotinamide adenine dinucleotide-linked dehydrogenase from a mutant of Escherichia coli capable of growth on 1, 2-propanediol. J Bacteriol. 1969 Apr;98(1):87–95. doi: 10.1128/jb.98.1.87-95.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tanaka S., Lerner S. A., Lin E. C. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol. 1967 Feb;93(2):642–648. doi: 10.1128/jb.93.2.642-648.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES