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. 1977 Dec;132(3):757–763. doi: 10.1128/jb.132.3.757-763.1977

Isolation of a lambdadcys transducing bacteriophage and its use in determining the regulation of cysteine messenger ribonucleic acid synthesis in Escherichia coli K-12.

A L Fimmel, R E Loughlin
PMCID: PMC235575  PMID: 336603

Abstract

A defective specialized lambda transducing phage carrying the cysJ, cysI, cysH, and cysD genes has been isolated from a secondary-site lysogen. Deoxyribonucleic acid-ribonucleic acid (DNA-RNA) hybridization studies utilizing this phage have been carried out to detect cysteine-specific messenger RNA (cys mRNA) synthesized in vivo. A vivo. A 3.5- to 9-fold increase in the rate of synthesis of cys mRNA has been detected in the derepressed wild-type (Cys+) strain grown on glutathione compared with a repressed control grown on cystine. Pleiotropic cysE and cysB mutants grown on glutathione were found to possess rates of synthesis of cys mRNA that were significantly lower than their derepressed isogenic parent. The addition of O-acetyl-L-serine to the cysE strain produced a 5.5-fold increase in the rate of synthesis of cys mRNA. These results indicate that cysteine biosynthesis is controlled at the level of transcription by the inducer O-acetylserine, the cysB protein and cyst(e)ine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheney R. W., Jr, Kredich N. M. Fine-structure genetic map of the cysB locus in Salmonella typhimurium. J Bacteriol. 1975 Dec;124(3):1273–1281. doi: 10.1128/jb.124.3.1273-1281.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DEMEREC M., GILLESPIE D. H., MIZOBUCHI K. GENETIC STRUCTURE OF THE CYST REGION OF THE SALMONELLA GENOME. Genetics. 1963 Aug;48:997–1009. doi: 10.1093/genetics/48.8.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  5. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  6. Hulanicka M. D., Kredich N. M., Treiman D. M. The structural gene for O-acetylserine sulfhydrylase A in Salmonella typhimurium. Identity with the trzA locus. J Biol Chem. 1974 Feb 10;249(3):867–872. [PubMed] [Google Scholar]
  7. Imamoto F., Yanofsky C. Transcription of the tryptophan operon in polarity mutants of Escherichia coli. I. Characterization of the tryptophan messenger RNA of polar mutants. J Mol Biol. 1967 Aug 28;28(1):1–23. doi: 10.1016/s0022-2836(67)80073-1. [DOI] [PubMed] [Google Scholar]
  8. Jones-Mortimer M. C. Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12. Biochem J. 1968 Dec;110(3):589–595. doi: 10.1042/bj1100589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jones-Mortimer M. C. Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12. Biochem J. 1968 Dec;110(3):597–602. doi: 10.1042/bj1100597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones-Mortimer M. C., Wheldrake J. F., Pasternak C. A. The control of sulphate reduction in Escherichia coli by O-acetyl-L-serine. Biochem J. 1968 Mar;107(1):51–53. doi: 10.1042/bj1070051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kikuchi A., Elseviers D., Gorini L. Isolation and characterization of lambda transducing bacteriophages for argF, argI and adjacent genes. J Bacteriol. 1975 May;122(2):727–742. doi: 10.1128/jb.122.2.727-742.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kredich N. M. Regulation of L-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth of varying sulfur sources and O-acetyl-L-serine on gene expression. J Biol Chem. 1971 Jun 10;246(11):3474–3484. [PubMed] [Google Scholar]
  13. Kredich N. M., Tomkins G. M. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. J Biol Chem. 1966 Nov 10;241(21):4955–4965. [PubMed] [Google Scholar]
  14. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  15. Loughlin R. E. Identification of nonsense mutations in the cysJ gene of Salmonella typhimurium. J Gen Microbiol. 1976 Jul;95(1):186–187. doi: 10.1099/00221287-95-1-186. [DOI] [PubMed] [Google Scholar]
  16. Loughlin R. E. Polarity of the cysJIH operon of Salmonella typhimurium. J Gen Microbiol. 1975 Feb;86(2):275–282. doi: 10.1099/00221287-86-2-275. [DOI] [PubMed] [Google Scholar]
  17. OKAMOTO K., SUGINO Y., NOMURA M. Synthesis and turnover of phage messenger RNA in E. coli infected with bacteriophage T4 in the presence of chloromycetin. J Mol Biol. 1962 Nov;5:527–534. doi: 10.1016/s0022-2836(62)80126-0. [DOI] [PubMed] [Google Scholar]
  18. PASTERNAK C. A., ELLIS R. J., JONES-MORTIMER M. C., CRICHTON C. E. THE CONTROL OF SULPHATE REDUCTION IN BACTERIA. Biochem J. 1965 Jul;96:270–275. doi: 10.1042/bj0960270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rose J. K., Mosteller R. D., Yanofsky C. Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970 Aug;51(3):541–550. doi: 10.1016/0022-2836(70)90007-0. [DOI] [PubMed] [Google Scholar]
  20. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  21. Steege D. A., Low B. Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli. J Bacteriol. 1975 Apr;122(1):120–128. doi: 10.1128/jb.122.1.120-128.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Szmelcman S., Hofnung M. Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor. J Bacteriol. 1975 Oct;124(1):112–118. doi: 10.1128/jb.124.1.112-118.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tully M., Yudkin M. D. The nature of the product of the cys B gene of Escherichia coli. Mol Gen Genet. 1975;136(2):181–183. doi: 10.1007/BF00272038. [DOI] [PubMed] [Google Scholar]
  24. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  25. Varmus H. E., Perlman R. L., Pastan I. Regulation of lac messenger ribonucleic acid synthesis by cyclic adenosine 3',5'-monophosphate and glucose. J Biol Chem. 1970 May 10;245(9):2259–2267. [PubMed] [Google Scholar]
  26. Wilcox G., Singer J., Heffernan L. Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r. J Bacteriol. 1971 Oct;108(1):1–4. doi: 10.1128/jb.108.1.1-4.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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