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. 1977 Dec;132(3):967–973. doi: 10.1128/jb.132.3.967-973.1977

Ultrastructural localization of alkaline phosphatase in the blue-green bacterium Plectonema boryanum.

B B Doonan, T E Jensen
PMCID: PMC235599  PMID: 411786

Abstract

Histochemical techniques applied at the ultrastructural level have clearly established the periplasmic space as the site of alkaline phosphatase activity in Plectonema boryanum. Considerable enzyme activity is found after the organism is placed in a phosphate-free medium for 5 days. This activity is found only in the cellular fraction of the culture with no activity present in the culture medium. Localization of the site of enzyme activity in cells was investigated by a modification of the method of Costerton. Unfixed cells were reacted with calcium nitrate, which acts as the initial capture reagent. After this deposition, the cells were suspended in 2% lead nitrate to convert the calcium phosphate to more electron-dense lead phosphate. The majority of activity appears associated with layer 3 (periplasmic space) of the cell wall.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhatti A. R., DeVoe I. W., Ingram J. M. The release and characterization of some periplasm-located enzymes of Pseudomona aeruginosa. Can J Microbiol. 1976 Oct;22(10):1425–1429. doi: 10.1139/m76-211. [DOI] [PubMed] [Google Scholar]
  2. Bone D. H. Relationship between phosphates and alkaline phosphatase of Anabaena flos-aquae in continuous culture. Arch Mikrobiol. 1971;80(2):147–153. doi: 10.1007/BF00411879. [DOI] [PubMed] [Google Scholar]
  3. Cashel M., Freese E. Excretion of alkaline phosphatase of Bacillus subtilis. Biochem Biophys Res Commun. 1964 Aug 11;16(6):541–544. doi: 10.1016/0006-291x(64)90189-5. [DOI] [PubMed] [Google Scholar]
  4. Cheng K. J., Ingram J. M., Costerton J. W. Alkaline phosphatase localization and spheroplast formation of Pseudomonas aeruginosa. Can J Microbiol. 1970 Dec;16(12):1319–1324. doi: 10.1139/m70-218. [DOI] [PubMed] [Google Scholar]
  5. Costerton J. W. Relationship of a wall-associated enzyme with specific layers of the cell wall of a gram-negative bacterium. J Bacteriol. 1973 Jun;114(3):1281–1293. doi: 10.1128/jb.114.3.1281-1293.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HORIUCHI T., HORIUCHI S., MIZUNO D. A possible negative feedback phenomenon controlling formation of alkaline phosphomonoesterase in Escherichia coli. Nature. 1959 May 30;183(4674):1529–1530. doi: 10.1038/1831529b0. [DOI] [PubMed] [Google Scholar]
  7. Ihlenfeldt M. J., Gibson J. Phosphate utilization and alkaline phosphatase activity in Anacystis nidulans (Synechococcus). Arch Microbiol. 1975;102(1):23–28. doi: 10.1007/BF00428340. [DOI] [PubMed] [Google Scholar]
  8. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MALAMY M., HORECKER B. L. The localization of alkaline phosphatase in E. coli K12. Biochem Biophys Res Commun. 1961 Jun 2;5:104–108. doi: 10.1016/0006-291x(61)90020-1. [DOI] [PubMed] [Google Scholar]
  10. MacaAlister T. J., Irvin R. T., Costerton J. W. Cell surface-localized alkaline phosphatase of Escherichia coli as visualized by reaction product deposition and ferritin-labeled antibodies. J Bacteriol. 1977 Apr;130(1):318–328. doi: 10.1128/jb.130.1.318-328.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McNicholas J. M., Hulett F. M. Electron microscope histochemical localization of alkaline phosphatase(s) in Bacillus licheniformis. J Bacteriol. 1977 Jan;129(1):501–515. doi: 10.1128/jb.129.1.501-515.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  13. Wetzel B. K., Spicer S. S., Dvorak H. F., Heppel L. A. Cytochemical localization of certain phosphatases in Escherichia coli. J Bacteriol. 1970 Oct;104(1):529–542. doi: 10.1128/jb.104.1.529-542.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wolk C. P. Physiology and cytological chemistry blue-green algae. Bacteriol Rev. 1973 Mar;37(1):32–101. doi: 10.1128/br.37.1.32-101.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wood D. A., Tristram H. Localization in the Cell and Extraction of Alkaline Phosphatase from Bacillus subtilis. J Bacteriol. 1970 Dec;104(3):1045–1051. doi: 10.1128/jb.104.3.1045-1051.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. ZEHNDER A., GORHAM P. R. Factors influencing the growth of Microcystis aeruginosa Kutz, emend, Elenkin. Can J Microbiol. 1960 Dec;6:645–660. doi: 10.1139/m60-077. [DOI] [PubMed] [Google Scholar]

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