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. 1977 Dec;132(3):1036–1037. doi: 10.1128/jb.132.3.1036-1037.1977

Selection of yeast auxotrophs by thymidylate starvation.

B J Barclay, J G Little
PMCID: PMC235609  PMID: 336601

Abstract

A rapid procedure for the recovery of Saccharomyces cerevisiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Henry S. A., Donahue T. F., Culbertson M. R. Selection of spontaneous mutants by inositol starvation in yeast. Mol Gen Genet. 1975 Dec 30;143(1):5–11. doi: 10.1007/BF00269415. [DOI] [PubMed] [Google Scholar]

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