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. 1975 Apr;122(1):47–53. doi: 10.1128/jb.122.1.47-53.1975

Growth of Pseudomonas C on C1 compounds: enzyme activites in extracts of Pseudomonas C cells grown on methanol, formaldehyde, and formate as sole carbon sources.

I Goldberg, R I Mateles
PMCID: PMC235637  PMID: 235511

Abstract

Pseudomonas C can grow on methanol, formaldehyde, or formate as sole carbon source. It is proposed that the assimilation of carbon by Pseudomonas C grown on different C1 growth substrates proceeds via one of two metabolic pathways, the serine pathway or the allulose pathway (the ribose phosphate cycle of formaldehyde fixation). This contention is based on the distribution of two key enzymes, each of which appears to be specifically involved in one of the assimilation pathways, glycerate dehydrogenase (serine pathway) and hexose phosphate synthetase (allulose pathway). The assimilation of methanol in Pseudomonas C cells appears to occur via the allulose pathway, whereas the utilization of formaldehyde or formate in cells grown on formaldehyde or formate as sole carbon sources appears by the serine pathway. When methanol is present together with formaldehyde or formate in the growth medium, the formaldehyde or formate is utilized by the allulose pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battat E., Goldberg I., Mateles R. I. Growth of Pseudomonas C on C1 compounds: continuoous culture. Appl Microbiol. 1974 Dec;28(6):906–911. doi: 10.1128/am.28.6.906-911.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chalfan Y., Mateles R. I. New pseudomonad utilizing methanol for growth. Appl Microbiol. 1972 Jan;23(1):135–140. doi: 10.1128/am.23.1.135-140.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colby J., Zatman L. J. Hexose phosphate synthese and tricarboxylic acid-cycle enzymes in bacterium 4B6, an obligate methylotroph. Biochem J. 1972 Aug;128(5):1373–1376. doi: 10.1042/bj1281373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooney C. L., Levine D. W. Microbial utilization of methanol. Adv Appl Microbiol. 1972;15:337–365. doi: 10.1016/s0065-2164(08)70096-0. [DOI] [PubMed] [Google Scholar]
  5. Dahl J. S., Mehta R. J., Hoare D. S. New obligate methylotroph. J Bacteriol. 1972 Feb;109(2):916–921. doi: 10.1128/jb.109.2.916-921.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kemp M. B., Quayle J. R. Microbial growth on C1 compounds. Incorporation of C1 units into allulose phosphate by extracts of Pseudomonas methanica. Biochem J. 1966 Apr;99(1):41–48. doi: 10.1042/bj0990041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kemp M. B., Quayle J. R. Microbial growth on C1 compounds. Uptake of [14C]formaldehyde and [14C]formate by methane-grown Pseudomonas methanica and determination of the hexose labelling pattern after brief incubation with [14C]methanol. Biochem J. 1967 Jan;102(1):94–102. doi: 10.1042/bj1020094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LARGE P. J., PEEL D., QUAYLE J. R. Microbial growth on C1 compounds. II. Synthesis of cell constituents by methanol- and formate-grown Pseudomonas AM 1, and methanol-grown Hyphomicrobium vulgare. Biochem J. 1961 Dec;81:470–480. doi: 10.1042/bj0810470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Large P. J., Quayle J. R. Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1. Biochem J. 1963 May;87(2):386–396. doi: 10.1042/bj0870386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lawrence A. J., Kemp M. B., Quayle J. R. Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans. Biochem J. 1970 Feb;116(4):631–639. doi: 10.1042/bj1160631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lawrence A. J., Quayle J. R. Alternative carbon assimilation pathways in methane-utilizing bacteria. J Gen Microbiol. 1970 Nov;63(3):371–374. doi: 10.1099/00221287-63-3-371. [DOI] [PubMed] [Google Scholar]
  13. Ribbons D. W., Harrison J. E., Wadzinski A. M. Metabolism of single carbon compounds. Annu Rev Microbiol. 1970;24:135–158. doi: 10.1146/annurev.mi.24.100170.001031. [DOI] [PubMed] [Google Scholar]
  14. STAFFORD H. A., MAGALDI A., VENNESLAND B. The enzymatic reduction of hydroxypyruvic acid to D-glyceric acid in higher plants. J Biol Chem. 1954 Apr;207(2):621–629. [PubMed] [Google Scholar]
  15. Stieglitz B., Mateles R. I. Methanol metabolism in pseudomonad C. J Bacteriol. 1973 Apr;114(1):390–398. doi: 10.1128/jb.114.1.390-398.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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