Abstract
Pseudomonas putida PpG6 is able to utilize purified n-alkanes of six to ten carbon atoms for growth. It can also grow on the primary terminal oxidation products of these alkanes and on 1-dodecanol but not on the corresponding 2-ketones or 1,6-hexanediol, adipic acid, or pimelic acid. Revertible point mutants can be isolated which have simultaneously lost the ability to grow on all five n-alkane growth substrates but which can still grow on octanol or nonanol. An acetate-negative mutant defective in isocitrate lysase activity is unable to grow on even-numbered alkanes and fatty acids. Analysis of double mutants defective in acetate and propionate or in acetate and glutarate metabolism shows that alkane carbon is assimilated only via acetyl-coenzyme A and propionyl-coenzyme A. These results support the following conclusions: (i) The n-alkane growth specificity of P. putida PpG6 is due to the substrate specificity of whole-cell alkane hydroxylation; (ii) there is a single alkane hydroxylase enzyme complex; (iii) the physiological role of this complex is to initiate the monoterminal oxidation of alkane chains; and (iv) straight-chain fatty acids from butyric through nonanoic are degraded exclusively by beta-oxidation from the carboxyl end of the molecule.
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- ALIKHAN M. Y., HALL A. N., ROBINSON D. S. PRODUCTS OF THE OXIDATION OF SELECTED ALKANES BY A GRAM-NEGATIVE BACTERIUM. Antonie Van Leeuwenhoek. 1964;30:417–427. doi: 10.1007/BF02046755. [DOI] [PubMed] [Google Scholar]
- BAPTIST J. N., GHOLSON R. K., COON M. J. Hydrocarbon oxidation by a bacterial enzyme system. I. Products of octane oxidation. Biochim Biophys Acta. 1963 Jan 1;69:40–47. doi: 10.1016/0006-3002(63)91223-x. [DOI] [PubMed] [Google Scholar]
- Cerdá-Olmedo E., Hanawalt P. C., Guerola N. Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome. J Mol Biol. 1968 May 14;33(3):705–719. doi: 10.1016/0022-2836(68)90315-x. [DOI] [PubMed] [Google Scholar]
- Chakrabarty A. M., Chou G., Gunsalus I. C. Genetic regulation of octane dissimilation plasmid in Pseudomonas. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1137–1140. doi: 10.1073/pnas.70.4.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GHOLSON R. K., BAPTIST J. N., COON M. J. HYDROCARBON OXIDATION BY A BACTERIAL ENZYME SYSTEM. II. COFACTOR REQUIREMENTS FOR OCTANOL FORMATION FROM OCTANE. Biochemistry. 1963 Sep-Oct;2:1155–1159. doi: 10.1021/bi00905a043. [DOI] [PubMed] [Google Scholar]
- HERINGA J. W., HUYBREGTSE R., van der LINDEN A. n-Alkane oxidation by a Pseudomonas. Formation and beta-oxidation of intermediate fatty acids. Antonie Van Leeuwenhoek. 1961;27:51–58. doi: 10.1007/BF02538422. [DOI] [PubMed] [Google Scholar]
- KUSUNOSE M., KUSUNOSE E., COON M. J. ENZYMATIC OMEGA-OXIDATION OF FATTY ACIDS. II. SUBSTRATE SPECIFICITY AND OTHER PROPERTIES OF THE ENZYME SYSTEM. J Biol Chem. 1964 Jul;239:2135–2139. [PubMed] [Google Scholar]
- McKenna E. J., Coon M. J. Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans. J Biol Chem. 1970 Aug 10;245(15):3882–3889. [PubMed] [Google Scholar]
- Peterson J. A., Kusunose M., Kusunose E., Coon M. J. Enzymatic omega-oxidation. II. Function of rubredoxin as the electron carrier in omega-hydroxylation. J Biol Chem. 1967 Oct 10;242(19):4334–4340. [PubMed] [Google Scholar]
- ROBINSON D. S. OXIDATION OF SELECTED ALKANES AND RELATED COMPOUNDS BY A PSEUDOMONAS STRAIN. Antonie Van Leeuwenhoek. 1964;30:303–316. doi: 10.1007/BF02046736. [DOI] [PubMed] [Google Scholar]
- Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
- THIJSSE G. J., van der LINDEN A. Iso-alkane oxidation by a Pseudomonas. I. Metabolism of 2-methylhexane. Antonie Van Leeuwenhoek. 1961;27:171–179. doi: 10.1007/BF02538437. [DOI] [PubMed] [Google Scholar]
- THIJSSE G. J., van der LINDEN A. Pathways of hydrocarbon dissimilation by a Pseudomonas as revealed by chloramphenicol. Antonie Van Leeuwenhoek. 1963;29:89–100. doi: 10.1007/BF02046042. [DOI] [PubMed] [Google Scholar]