Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Jul;123(1):20–27. doi: 10.1128/jb.123.1.20-27.1975

Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1.

P L Shipley, R H Olsen
PMCID: PMC235686  PMID: 1095551

Abstract

A plasmid segregant carrying tetracycline and carbenicillin resistance markers has been isolated from R factor RP1 by transductional shortening with phage P22. The new plasmid RP1-S2, which has a molecular weight of 23 times 10-6, has lost the transfer, phage sensitivity, and neomycin resistance functions of RP1. It combines readily with a W group plasmid, R388, to form a transmissible carbenicillin and trimethoprim resistance plasmid, RWP1.

Full text

PDF
20

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Natkin E. Transduction of resistance determinants and R factors of the transfer systems by phage Plkc. Mol Gen Genet. 1972;114(3):261–265. doi: 10.1007/BF01788895. [DOI] [PubMed] [Google Scholar]
  2. Botstein D., Chan R. K., Waddell C. H. Genetics of bacteriophage P22. II. Gene order and gene function. Virology. 1972 Jul;49(1):268–282. doi: 10.1016/s0042-6822(72)80028-x. [DOI] [PubMed] [Google Scholar]
  3. Chan R. K., Botstein D. Genetics of bacteriophage P22. I. Isolation of prophage deletions which affect immunity to superinfection. Virology. 1972 Jul;49(1):257–267. doi: 10.1016/s0042-6822(72)80027-8. [DOI] [PubMed] [Google Scholar]
  4. Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coetzee J. N., Datta N., Hedges R. W., Appelbaum P. C. Transduction of R factors in Proteus mirabilis and P. rettgeri. J Gen Microbiol. 1973 Jun;76(2):355–368. doi: 10.1099/00221287-76-2-355. [DOI] [PubMed] [Google Scholar]
  6. Cohen S. N., Chang A. C. Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc Natl Acad Sci U S A. 1973 May;70(5):1293–1297. doi: 10.1073/pnas.70.5.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cohen S. N., Miller C. A. Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. J Mol Biol. 1970 Jun 28;50(3):671–687. doi: 10.1016/0022-2836(70)90092-6. [DOI] [PubMed] [Google Scholar]
  8. DUBNAU E., STOCKER B. A. GENETICS OF PLASMIDS IN SALMONELLA TYPHIMURIUM. Nature. 1964 Dec 12;204:1112–1113. doi: 10.1038/2041112a0. [DOI] [PubMed] [Google Scholar]
  9. Falkow S., Citarella R. V., Wohlhieter J. A. The molecular nature of R-factors. J Mol Biol. 1966 May;17(1):102–116. doi: 10.1016/s0022-2836(66)80097-9. [DOI] [PubMed] [Google Scholar]
  10. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hedges R. W., Jacob A. E. Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet. 1974;132(1):31–40. doi: 10.1007/BF00268228. [DOI] [PubMed] [Google Scholar]
  14. KONDO E., HARADA K., MITSUHASHI S. Drug-resistance of enteric bacteria. 12. Transduction of the transmissible drug resistance factor by bacteriophage Plkc. Jpn J Exp Med. 1962 Feb;32:139–147. [PubMed] [Google Scholar]
  15. Kameda M., Harada K., Suzuki M., Mitsuhashi S. Drug resistance of enteric bacteria. V. High frequency of transduction of R factors with bacteriophage epsilon. J Bacteriol. 1965 Nov;90(5):1174–1181. doi: 10.1128/jb.90.5.1174-1181.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Olsen R. H., Gonzalez C. Escherichia coli gene transfer to unrelated bacteria by a histidine operon--RP1 drug resistance plasmic complex. Biochem Biophys Res Commun. 1974 Jul 10;59(1):377–385. doi: 10.1016/s0006-291x(74)80217-2. [DOI] [PubMed] [Google Scholar]
  17. Olsen R. H., Shipley P. L. RP1 properties and fertility inhibition among P, N, W, and X incompatibility group plasmids. J Bacteriol. 1975 Jul;123(1):28–35. doi: 10.1128/jb.123.1.28-35.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Olsen R. H., Shipley P. Host range and properties of the Pseudomonas aeruginosa R factor R1822. J Bacteriol. 1973 Feb;113(2):772–780. doi: 10.1128/jb.113.2.772-780.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Olsen R. H., Siak J. S., Gray R. H. Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. J Virol. 1974 Sep;14(3):689–699. doi: 10.1128/jvi.14.3.689-699.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Olsen R. H., Thomas D. D. Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid. J Virol. 1973 Dec;12(6):1560–1567. doi: 10.1128/jvi.12.6.1560-1567.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pemberton J. M., Clark A. J. Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. J Bacteriol. 1973 Apr;114(1):424–433. doi: 10.1128/jb.114.1.424-433.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rhoades M., MacHattie L. A., Thomas C. A., Jr The P22 bacteriophage DNA molecule. I. The mature form. J Mol Biol. 1968 Oct 14;37(1):21–40. doi: 10.1016/0022-2836(68)90071-5. [DOI] [PubMed] [Google Scholar]
  23. Sharp P. A., Cohen S. N., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol. 1973 Apr 5;75(2):235–255. doi: 10.1016/0022-2836(73)90018-1. [DOI] [PubMed] [Google Scholar]
  24. Shipley P. L., Olsen R. H. Characteristics and expression of tetracycline resistance in gram-negative bacteria carrying the Pseudomonas R factor RP1. Antimicrob Agents Chemother. 1974 Aug;6(2):183–190. doi: 10.1128/aac.6.2.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Spratt B. G., Rowbury R. J. The plasmid of Salmonella typhimurium LT2. Mol Gen Genet. 1973 Mar 19;121(4):347–353. doi: 10.1007/BF00433233. [DOI] [PubMed] [Google Scholar]
  26. Tye B. K., Chan R. K., Botstein D. Packaging of an oversize transducing genome by Salmonella phage P22. J Mol Biol. 1974 Jan 5;85(4):485–500. doi: 10.1016/0022-2836(74)90311-8. [DOI] [PubMed] [Google Scholar]
  27. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  28. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae. III. Transduotion of resistance factors. J Bacteriol. 1961 Aug;82:202–209. doi: 10.1128/jb.82.2.202-209.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Watanabe T., Ogata Y. Abortive transduction of resistance factor by bacteriophage P22 in Salmonella typhimurium. J Bacteriol. 1970 May;102(2):596–597. doi: 10.1128/jb.102.2.596-597.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Watanabe T., Ogata Y., Chan R. K., Botstein D. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22. Virology. 1972 Dec;50(3):874–882. doi: 10.1016/0042-6822(72)90441-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES