Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Jul;123(1):36–40. doi: 10.1128/jb.123.1.36-40.1975

Are thiosulfate and trithionate intermediates in dissimilatory sulfate reduction?

L A Chambers, P A Trudinger
PMCID: PMC235688  PMID: 1141200

Abstract

The fate of 35-S during anaerobic metabolism of [35-S]sulfate, [35-S]thiosulfate, and [35-S]sulfate plus unlabeled thiosulfate by washed cell suspensions of Desulfovibrio spp, and of [35-S]thiosulfate by growing D. desulfuricans was examined. The results appear to be inconsistent with the hypothesis that thiosulfate is an intermediate in sulfate reduction. Since thiosulfate was produced from trithionate, the latter is also unlikely to be an intermediate in the reduction pathway. Extracts of D. desulfuricans catalysed exchange between sulfite and the sulfonate group of thiosulfate.

Full text

PDF
36

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akagi J. M., Adams V. Isolation of a bisulfite reductase activity from Desulfotomaculum nigrificans and its identification as the carbon monoxide-binding pigment P582. J Bacteriol. 1973 Oct;116(1):392–396. doi: 10.1128/jb.116.1.392-396.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akagi J. M., Chan M., Adams V. Observations on the bisulfite reductase (P582) isolated from Desulfotomaculum nigrificans. J Bacteriol. 1974 Oct;120(1):240–244. doi: 10.1128/jb.120.1.240-244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELLMAN G. L. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959 May;82(1):70–77. doi: 10.1016/0003-9861(59)90090-6. [DOI] [PubMed] [Google Scholar]
  4. Findley J. E., Akagi J. M. Evidence for thiosulfate formation during sulfite reduction by Desulfovibrio vulgaris. Biochem Biophys Res Commun. 1969 Jul 23;36(2):266–271. doi: 10.1016/0006-291x(69)90324-6. [DOI] [PubMed] [Google Scholar]
  5. Findley J. E., Akagi J. M. Role of thiosulfate in bisulfite reduction as catalyzed by Desulfovibrio vulgaris. J Bacteriol. 1970 Sep;103(3):741–744. doi: 10.1128/jb.103.3.741-744.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Haschke R. H., Campbell L. L. Thiosulfate reductase of Desulfovibrio vulgaris. J Bacteriol. 1971 May;106(2):603–607. doi: 10.1128/jb.106.2.603-607.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones H. E., Skyring G. W. Reduction of sulphite to sulphide catalysed by desulfoviridin from Desulfovibrio gigas. Aust J Biol Sci. 1974 Feb;27(1):7–14. [PubMed] [Google Scholar]
  8. Jones H. E. Sulfate-reducing bacterium with unusual morphology and pigment content. J Bacteriol. 1971 May;106(2):339–346. doi: 10.1128/jb.106.2.339-346.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kelly D. P., Syrett P. J. [35S]thiosulphate oxidation by Thiobacillus strain C. Biochem J. 1966 Feb;98(2):537–545. doi: 10.1042/bj0980537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kobayashi K., Seki Y., Ishimoto M. Biochemical studies on sulfate-ruducing bacteria. 8. Sulfite reductase from Desulfovibrio vulgaris--mechanism of trithionate, thiosulfate, and sulfide formation and enzymatic properties. J Biochem. 1974 Mar;75(3):519–529. doi: 10.1093/oxfordjournals.jbchem.a130420. [DOI] [PubMed] [Google Scholar]
  11. Kobayashi K., Tachibana S., Ishimoto M. Intermediary formation of trithionate in sulfite reduction by a sulfate-reducing bacterium. J Biochem. 1969 Jan;65(1):155–157. [PubMed] [Google Scholar]
  12. Kobayashi K., Takahashi E., Ishimoto M. Biochemical studies on sulfate-reducing bacteria. XI. Purification and some properties of sulfite reductase, desulfoviridin. J Biochem. 1972 Oct;72(4):879–887. doi: 10.1093/oxfordjournals.jbchem.a129982. [DOI] [PubMed] [Google Scholar]
  13. Kredich N. M., Tomkins G. M. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. J Biol Chem. 1966 Nov 10;241(21):4955–4965. [PubMed] [Google Scholar]
  14. Lee J. P., LeGall J., Peck H. D., Jr Isolation of assimilatroy- and dissimilatory-type sulfite reductases from Desulfovibrio vulgaris. J Bacteriol. 1973 Aug;115(2):529–542. doi: 10.1128/jb.115.2.529-542.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lee J. P., Peck H. D., Jr Purification of the enzyme reducing bisulfite to trithionate from Desulfovibrio gigas and its identification as desulfoviridin. Biochem Biophys Res Commun. 1971 Nov 5;45(3):583–589. doi: 10.1016/0006-291x(71)90457-8. [DOI] [PubMed] [Google Scholar]
  16. Nakatsukasa W., Akagi J. M. Thiosulfate reductase isolated from Desulfotomaculum nigrificans. J Bacteriol. 1969 May;98(2):429–433. doi: 10.1128/jb.98.2.429-433.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. POSTGATE J. R. The reduction of sulphur compounds by Desulphovibrio desulphuricans. J Gen Microbiol. 1951 Oct;5(4):725–738. doi: 10.1099/00221287-5-4-725. [DOI] [PubMed] [Google Scholar]
  18. Suh B., Akagi J. M. Formation of thiosulfate from sulfite by Desulfovibrio vulgaris. J Bacteriol. 1969 Jul;99(1):210–215. doi: 10.1128/jb.99.1.210-215.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Trudinger P. A. On the absorbancy of reduced methyl viologen. Anal Biochem. 1970 Jul;36(1):222–225. doi: 10.1016/0003-2697(70)90351-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES