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. 1975 Aug;123(2):436–442. doi: 10.1128/jb.123.2.436-442.1975

Evidence for cytochrome involvement in fumarate reduction and adenosine 5'-triphosphate synthesis by Bacteroides fragilis grown in the presence of hemin.

J Macy, I Probst, G Gottschalk
PMCID: PMC235746  PMID: 1150622

Abstract

Growth of Bacteroides fragilis subsp. fragilis on glucose was very much stimulated by the addition of hemin (2 mg/liter) to the medium. The generation time decreased from 8 to 2 h, and the molar growth yield increased from YM = 17.9 to YM = 47 g (dry weight) of cells per mol of glucose. In the absence of hemin, glucose was fermented to fumarate, lactate, and acetate. The cells did not contain detectable amounts of cytochromes or fumarate reductase. In the presence of hemin, the major products of fermentation were succinate, propionate, and acetate. A b-type cytochrome, possibly a c-type cytochrome, and a very active fumarate reductase were present in the cells. It is concluded from these results that hemin is required by B. fragilis to synthesize a functional fumarate reductase and that the hemin-dependent, enormous increase of the growth yield may be due to adenosine 5'-triphosphate production during reduction of fumarate to succinate.

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Selected References

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  1. Anderson R. L., Wood W. A. Carbohydrate metabolism in microorganisms. Annu Rev Microbiol. 1969;23:539–578. doi: 10.1146/annurev.mi.23.100169.002543. [DOI] [PubMed] [Google Scholar]
  2. BALDWIN R. L., MILLIGAN L. P. ELECTRON TRANSPORT IN PEPTOSTREPTOCOCCUS ELSDENII. Biochim Biophys Acta. 1964 Dec 23;92:421–432. doi: 10.1016/0926-6569(64)90001-x. [DOI] [PubMed] [Google Scholar]
  3. BAUCHOP T., ELSDEN S. R. The growth of micro-organisms in relation to their energy supply. J Gen Microbiol. 1960 Dec;23:457–469. doi: 10.1099/00221287-23-3-457. [DOI] [PubMed] [Google Scholar]
  4. BRYANT M. P., ROBINSON I. M. Some nutritional characteristics of predominant culturable ruminal bacteria. J Bacteriol. 1962 Oct;84:605–614. doi: 10.1128/jb.84.4.605-614.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barton L. L., Le Gall J., Peck H. D., Jr Phosphorylation coupled to oxidation of hydrogen with fumarate in extracts of the sulfate reducing bacterium, Desulfovibrio gigas. Biochem Biophys Res Commun. 1970 Nov 25;41(4):1036–1042. doi: 10.1016/0006-291x(70)90189-0. [DOI] [PubMed] [Google Scholar]
  6. Caldwell D. R., White D. C., Bryant M. P., Doetsch R. N. Specificity of the heme requirement for growth of Bacteroides ruminicola. J Bacteriol. 1965 Dec;90(6):1645–1654. doi: 10.1128/jb.90.6.1645-1654.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gottwald M., Andreesen J. R., LeGall J., Ljungdahl L. G. Presence of cytochrome and menaquinone in Clostridium formicoaceticum and Clostridium thermoaceticum. J Bacteriol. 1975 Apr;122(1):325–328. doi: 10.1128/jb.122.1.325-328.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hatchikian E. C., Le Gall J. Evidence for the presence of a b-type cytochrome in the sulfate-reducing bacterium Desulfovibrio gigas, and its role in the reduction of fumarate by molecular hydrogen. Biochim Biophys Acta. 1972 Jun 23;267(3):479–484. doi: 10.1016/0005-2728(72)90175-2. [DOI] [PubMed] [Google Scholar]
  9. JACOBS N. J., WOLIN M. J. Electron-transport system of Vibrio succinogenes. I. Enzymes and cytochromes of electron-transport system. Biochim Biophys Acta. 1963 Jan 1;69:18–28. doi: 10.1016/0006-3002(63)91221-6. [DOI] [PubMed] [Google Scholar]
  10. Kamen M. D., Horio T. Bacterial cytochromes. I. Structural aspects. Annu Rev Biochem. 1970;39:673–700. doi: 10.1146/annurev.bi.39.070170.003325. [DOI] [PubMed] [Google Scholar]
  11. Kröger A., Dadák V., Klingenberg M., Diemer F. On the role of quinones in bacterial electron transport. Differential roles of ubiquinone and menaquinone in Proteus rettgeri. Eur J Biochem. 1971 Aug 16;21(3):322–333. doi: 10.1111/j.1432-1033.1971.tb01472.x. [DOI] [PubMed] [Google Scholar]
  12. NEUFELD H. A., SCOTT C. R., STOTZ E. Purification of heart muscle succinic dehydrogenase. J Biol Chem. 1954 Oct;210(2):869–876. [PubMed] [Google Scholar]
  13. PECK H. D., Jr, SMITH O. H., GEST H. Comparative biochemistry of the biological reduction of fumaric acid. Biochim Biophys Acta. 1957 Jul;25(1):142–147. doi: 10.1016/0006-3002(57)90431-6. [DOI] [PubMed] [Google Scholar]
  14. Rizza V., Sinclair P. R., White D. C., Cuorant P. R. Electron transport system of the protoheme-requiring anaerobe Bacteroides melaninogenicus. J Bacteriol. 1968 Sep;96(3):665–671. doi: 10.1128/jb.96.3.665-671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sone N. The redox reactions in propionic acid fermantation. I. Occurrence and nature of an electron transfer system in Propionibacterium arabinosum. J Biochem. 1972 Jun;71(6):931–940. doi: 10.1093/oxfordjournals.jbchem.a129864. [DOI] [PubMed] [Google Scholar]
  16. Stouthamer A. H. A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie Van Leeuwenhoek. 1973;39(3):545–565. doi: 10.1007/BF02578899. [DOI] [PubMed] [Google Scholar]
  17. Stouthamer A. H., Bettenhaussen C. Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms. A reevaluation of the method for the determination of ATP production by measuring molar growth yields. Biochim Biophys Acta. 1973 Feb 12;301(1):53–70. doi: 10.1016/0304-4173(73)90012-8. [DOI] [PubMed] [Google Scholar]
  18. Varel V. H., Bryant M. P. Nutritional features of Bacteroides fragilis subsp. fragilis. Appl Microbiol. 1974 Aug;28(2):251–257. doi: 10.1128/am.28.2.251-257.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WHITE D. C., BRYANT M. P., CALDWELL D. R. Cytochromelinked fermentation in Bacteroides ruminicola. J Bacteriol. 1962 Oct;84:822–828. doi: 10.1128/jb.84.4.822-828.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wallnöfer P., Baldwin R. L. Pathway of propionate formation in Bacteroides ruminicola. J Bacteriol. 1967 Jan;93(1):504–505. doi: 10.1128/jb.93.1.504-505.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. de Vries W., Wijck-Kapteijn W. M., Stouthamer A. H. Influence of oxygen on growth, cytochrome synthesis and fermentation pattern in propionic acid bacteria. J Gen Microbiol. 1972 Aug;71(3):515–524. doi: 10.1099/00221287-71-3-515. [DOI] [PubMed] [Google Scholar]
  22. de Vries W., van Wijck-Kapteyn W. M., Oosterhuis S. K. The presence and function of cytochromes in Selenomonas ruminantium, Anaerovibrio lipolytica and Veillonella alcalescens. J Gen Microbiol. 1974 Mar;81(1):69–78. doi: 10.1099/00221287-81-1-69. [DOI] [PubMed] [Google Scholar]
  23. de Vries W., van Wyck-Kapteyn W. M., Stouthamer A. H. Generation of ATP during cytochrome-linked anaerobic electron transport in propionic acid bacteria. J Gen Microbiol. 1973 May;76(1):31–41. doi: 10.1099/00221287-76-1-31. [DOI] [PubMed] [Google Scholar]

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