Abstract
The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The results are in quantitative accordance with a random replication of R1 in which the replicating molecules are taken from a cytoplasmic plasmid pool and transferred back to the pool after replication. This is analogous to the results obtained by Bazaral and Helinski (1970) and Rownd (1969) for plasmids that are present in 10 to 20 copies per genome (so-called relaxed control of replication). Hence, there seem to be no difference between stringent and relaxed plasmids with respect to selection of plasmid molecules for replication. However, we cannot tell whether all R1 copies in a cell replicate during a fraction of or throughout the cell cycle. The random selction of plasmid copies for replication has to be considered when models for control of replication are constructed.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abe M. The replication of prophage P1 DNA. Mol Gen Genet. 1974;132(1):63–72. doi: 10.1007/BF00268231. [DOI] [PubMed] [Google Scholar]
- Bazaral M., Helinski D. R. Replication of a bacterial plasmid and an episome in Escherichia coli. Biochemistry. 1970 Jan 20;9(2):399–406. doi: 10.1021/bi00804a029. [DOI] [PubMed] [Google Scholar]
- Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collins J., Pritchard R. H. Relationship between chromosome replication and F'lac episome replication in Escherichia coli. J Mol Biol. 1973 Jun 25;78(1):143–155. doi: 10.1016/0022-2836(73)90434-8. [DOI] [PubMed] [Google Scholar]
- Cooper S. Relationship of Flac replication and chromosome replication. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2706–2710. doi: 10.1073/pnas.69.9.2706. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis D. B., Helmstetter C. E. Control of F'lac replication in Escherichia coli B-r. J Bacteriol. 1973 Apr;114(1):294–299. doi: 10.1128/jb.114.1.294-299.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ERRINGTON F. P., POWELL E. O., THOMPSON N. GROWTH CHARACTERISITICS OF SOME GRAM-NEGATIVE BACTERIA. J Gen Microbiol. 1965 Apr;39:109–123. doi: 10.1099/00221287-39-1-109. [DOI] [PubMed] [Google Scholar]
- Engberg B., Nordström K. Replication of R-factor R1 in Scherichia coli K-12 at different growth rates. J Bacteriol. 1975 Jul;123(1):179–186. doi: 10.1128/jb.123.1.179-186.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Falkow S., Tompkins L. S., Silver R. P., Guerry P., Le Blanc D. J. The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation. Ann N Y Acad Sci. 1971 Jun 11;182:153–171. doi: 10.1111/j.1749-6632.1971.tb30654.x. [DOI] [PubMed] [Google Scholar]
- Goebel W. The influence of DNA A and DNA C mutations on the initiation of plasmid DNA replication. Biochem Biophys Res Commun. 1973 Apr 16;51(4):1000–1007. doi: 10.1016/0006-291x(73)90026-0. [DOI] [PubMed] [Google Scholar]
- Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Helinski D. R. Plasmid determined resistance to antibiotics: molecular properties of R factors. Annu Rev Microbiol. 1973;27:437–470. doi: 10.1146/annurev.mi.27.100173.002253. [DOI] [PubMed] [Google Scholar]
- Kline B. C. Mechanism and biosynthetic requirements for F plasmid replication in Escherichia coli. Biochemistry. 1974 Jan 1;13(1):139–146. doi: 10.1021/bi00698a022. [DOI] [PubMed] [Google Scholar]
- Kubitschek H. E. Generation times: ancestral dependence and dependence upon cell size. Exp Cell Res. 1966 Aug;43(1):30–38. doi: 10.1016/0014-4827(66)90374-0. [DOI] [PubMed] [Google Scholar]
- Kupersztoch-Portnoy Y. M., Miklos G. L., Helinski D. R. Properties of the relaxation complexes of supercoiled deoxyribonucleic acid and protein of the R plasmids R64, R28K, and R6K. J Bacteriol. 1974 Oct;120(1):545–548. doi: 10.1128/jb.120.1.545-548.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meynell E., Datta N. Mutant drug resistant factors of high transmissibility. Nature. 1967 May 27;214(5091):885–887. doi: 10.1038/214885a0. [DOI] [PubMed] [Google Scholar]
- Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nordström K., Eriksson-Grennberg K. G., Boman H. G. Resistance of Escherichia coli to penicillins. 3. AmpB, a locus affecting episomally and chromosomally mediated resistance to ampicillin and chlorampheincol. Genet Res. 1968 Oct;12(2):157–168. doi: 10.1017/s0016672300011770. [DOI] [PubMed] [Google Scholar]
- Nordström K., Ingram L. C., Lundbäck A. Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome. J Bacteriol. 1972 May;110(2):562–569. doi: 10.1128/jb.110.2.562-569.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nordström U. M., Engberg B., Nordström K. Competition for DNA polymerase III between the chromosome and the R-factor R1. Mol Gen Genet. 1974;135(3):185–190. doi: 10.1007/BF00268614. [DOI] [PubMed] [Google Scholar]
- POWELL E. O., ERRINGTON F. P. Generation times of individual bacteria: some corroborative measurements. J Gen Microbiol. 1963 May;31:315–327. doi: 10.1099/00221287-31-2-315. [DOI] [PubMed] [Google Scholar]
- Pierucci O. Regulation of cell division in Escherichia coli. Biophys J. 1969 Jan;9(1):90–112. doi: 10.1016/S0006-3495(69)86371-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pritchard R. H. Review lecture on the growth and form of a bacterial cell. Philos Trans R Soc Lond B Biol Sci. 1974 Feb 21;267(886):303–336. doi: 10.1098/rstb.1974.0003. [DOI] [PubMed] [Google Scholar]
- Rownd R. Replication of a bacterial episome under relaxed control. J Mol Biol. 1969 Sep 28;44(3):387–402. doi: 10.1016/0022-2836(69)90368-4. [DOI] [PubMed] [Google Scholar]
- SCHAECHTER M., WILLIAMSON J. P., HOOD J. R., Jr, KOCH A. L. Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962 Nov;29:421–434. doi: 10.1099/00221287-29-3-421. [DOI] [PubMed] [Google Scholar]
- Thompson R., Broda P. DNA polymerase 3 and the replication of F and ColVBtrp in Escherichia coli K-12. Mol Gen Genet. 1973 Dec 31;127(3):255–258. doi: 10.1007/BF00333765. [DOI] [PubMed] [Google Scholar]
- Zeuthen J., Pato M. L. Replication of the F'lac sex factor in the cell cycle of Escherichia coli. Mol Gen Genet. 1971;111(3):242–255. doi: 10.1007/BF00433109. [DOI] [PubMed] [Google Scholar]