Abstract
During initiation of Bacillus megaterium QM B1551 spore germination, trichloroacetic acid-soluble, nondialyzable peptidoglycan fragments with an average molecular weight of 20,000 were excreted. This solubilization of peptidoglycan was measured in vitro as the amount of trichloroacetic acid-soluble hexosamine released from a suspension of broken spores. HgC12, a potent inhibitor of initiation, had no effect on the in vitro solubilization of peptidoglycan. In vivo, HgC12 had no effect on peptidoglycan release from spores that had lost heat resistance, but HgC12 did block complete absorbance loss. These results suggest that mercury inhibits some reactions that normally occur before loss in heat resistance but not the subsequent peptidoglycan release, and mercury inhibits other reactions involved with complete absorbance loss.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aronson A. I., Fitz-James P. C. Biosynthesis of bacterial spore coats. J Mol Biol. 1968 Apr 14;33(1):199–212. doi: 10.1016/0022-2836(68)90288-x. [DOI] [PubMed] [Google Scholar]
- Best G. K., Mattingly S. J. Chemical analysis of cell walls and autolytic digests of Bacillus psychrophilus. J Bacteriol. 1973 Jul;115(1):221–227. doi: 10.1128/jb.115.1.221-227.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fan D. P., Beckman M. M. Micrococcus lysodeikticus bacterial walls as a substrate specific for the autolytic glycosidase of Bacillus subtilis. J Bacteriol. 1973 May;114(2):804–813. doi: 10.1128/jb.114.2.804-813.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gould G. W., Hitchins A. D., King W. L. Function and location of a "germination enzyme" in spores of Bacillus cereus. J Gen Microbiol. 1966 Aug;44(2):293–502. doi: 10.1099/00221287-44-2-293. [DOI] [PubMed] [Google Scholar]
- Hashimoto T., Frieben W. R., Conti S. F. Microgermination of Bacillus cereus spores. J Bacteriol. 1969 Dec;100(3):1385–1392. doi: 10.1128/jb.100.3.1385-1392.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kondo M., Foster J. W. Chemical and electron microscope studies on fractions prepared from coats of Bacillus spores. J Gen Microbiol. 1967 May;47(2):257–271. doi: 10.1099/00221287-47-2-257. [DOI] [PubMed] [Google Scholar]
- Levinson H. S., Hyatt M. T. Sequence of events during Bacillus megaterim spore germination. J Bacteriol. 1966 May;91(5):1811–1818. doi: 10.1128/jb.91.5.1811-1818.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson D. L., Kornberg A. Biochemical studies of bacterial sporulation and germination. 18. Free amino acids in spores. J Biol Chem. 1970 Mar 10;245(5):1128–1136. [PubMed] [Google Scholar]
- POWELL J. F., STRANGE R. E. Biochemical changes occurring during the germination of bacterial spores. Biochem J. 1953 May;54(2):205–209. doi: 10.1042/bj0540205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RECORD B. R., GRINSTEAD K. H. Physico-chemical properties and molecular weight of spore peptides from Bacillus megatherium. Biochem J. 1954 Sep;58(1):85–87. [PubMed] [Google Scholar]
- STRANGE R. E., DARK F. A. A cell-wall lytic enzyme associated with spores of Bacillus species. J Gen Microbiol. 1957 Feb;16(1):236–249. doi: 10.1099/00221287-16-1-236. [DOI] [PubMed] [Google Scholar]
- STRANGE R. E., DARK F. A. Cell-wall lytic enzymes at sporulation and spore germination in Bacillus species. J Gen Microbiol. 1957 Oct;17(2):525–537. doi: 10.1099/00221287-17-2-525. [DOI] [PubMed] [Google Scholar]
- STRANGE R. E., POWELL J. F. Hexosamine-containing peptides in spores of Bacillus subtilis, B. megatherium and B. cereus. Biochem J. 1954 Sep;58(1):80–85. doi: 10.1042/bj0580080. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vary J. C. Germination of Bacillus megaterium spores after various extraction procedures. J Bacteriol. 1973 Nov;116(2):797–802. doi: 10.1128/jb.116.2.797-802.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vinter V. Symposium on bacterial spores: V. Germination and outgrowth: effect of inhibitors. J Appl Bacteriol. 1970 Mar;33(1):50–59. doi: 10.1111/j.1365-2672.1970.tb05233.x. [DOI] [PubMed] [Google Scholar]
- Warth A. D., Strominger J. L. Structure of the peptidoglycan from spores of Bacillus subtilis. Biochemistry. 1972 Apr 11;11(8):1389–1396. doi: 10.1021/bi00758a010. [DOI] [PubMed] [Google Scholar]