Abstract
Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall locus. The mn4 locus, which regulates the synthesis of mannosylphosphate groups on the mannan side chains, was placed on chromosome XI near trp3 and ural and a locus previously reported to regulate the ability of a S. diastaticus strain to bind alcian blue (Friis and Ottolenghi, 1970). The mnn4 mutant also fails to bind alcain blue, but the gene responsible for alcian blud binding in this strain segregates independently from the dye-binding locus of S. diastaticus, and therefore must be a different gene. A diploid heterozygous for mnn4 fails to bind dye, indicating dominance of this mutant genotype. The alcian blue dye binding locus dbll, reported to Friis and Ottolenghi (1970), is also dominant. Thus, there are at least two independent genes that control the formation of the mannosylphosphate units in the mannan side chains, and both have the property of dominance in the mutant form.
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Selected References
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- Antalis C., Fogel S., Ballou C. E. Genetic control of yeast mannan structure. Mapping the first gene concerned with mannan biosynthesis. J Biol Chem. 1973 Jul 10;248(13):4655–4659. [PubMed] [Google Scholar]
- Ballou C. E., Kern K. A., Raschke W. C. Genetic control of yeast mannan structure. Complementation studies and properties of mannan mutants. J Biol Chem. 1973 Jul 10;248(13):4667–4671. [PubMed] [Google Scholar]
- Ballou C. E., Raschke W. C. Polymorphism of the somatic antigen of yeast. Science. 1974 Apr 12;184(4133):127–134. doi: 10.1126/science.184.4133.127. [DOI] [PubMed] [Google Scholar]
- Ballou C. E. Some aspects of the structure, immunochemistry, and genetic control of yeast mannans. Adv Enzymol Relat Areas Mol Biol. 1974;40(0):239–270. doi: 10.1002/9780470122853.ch6. [DOI] [PubMed] [Google Scholar]
- Friis J., Ottolenghi P. The genetically determined binding of alcian blue by a minor fraction of yeast cell walls. C R Trav Lab Carlsberg. 1970;37(15):327–341. [PubMed] [Google Scholar]
- Nakajima T., Ballou C. E. Structure of the linkage region between the polysaccharide and protein parts of Saccharomyces cerevisiae mannan. J Biol Chem. 1974 Dec 10;249(23):7685–7694. [PubMed] [Google Scholar]
- Raschke W. C., Kern K. A., Antalis C., Ballou C. E. Genetic control of yeast mannan structure. Isolation and characterization of mannan mutants. J Biol Chem. 1973 Jul 10;248(13):4660–4666. [PubMed] [Google Scholar]