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. 1975 Aug;123(2):637–641. doi: 10.1128/jb.123.2.637-641.1975

Derepression in Saccharomyces cerevisiae can be dissociated from cellular proliferation and deoxyribonucleic acid synthesis.

H R Mahler, K Assimos, C C Lin
PMCID: PMC235770  PMID: 1099068

Abstract

A method has been developed that permits precise control of release from catabolite repression in Saccharomyces cerevisiae. It consists of transferring cells growing exponentially on 5% glucose to derepression medium at high cell density. Derepression then proceeds with reproducible kinetics and is complete within 6 to 7.5 h for various intra- and extramitochondrial markers, in the absence of any substantial increase in cellular dry weight or protein. Nuclear (and mitochondrial) deoxyribonucleic acid synthesis can be interrupted in certain thermosensitive (cdc) mutants at the nonpermissive temperature; a shift to this temperature before the onset of derepression has no effect on its outcome.

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Selected References

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  1. Barath Z., Küntzel H. Cooperation of mitochondrial and nuclear genes specifying the mitochondrial genetic apparatus in Neurospora crassa. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1371–1374. doi: 10.1073/pnas.69.6.1371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barath Z., Küntzel H. Induction of mitochondrial RNA polymerase in Neurospora crassa. Nat New Biol. 1972 Dec 13;240(102):195–197. doi: 10.1038/newbio240195a0. [DOI] [PubMed] [Google Scholar]
  3. Cottrell S., Rabinowitz M., Getz G. S. Mitochondrial deoxyribonucleic acid synthesis in a temperature-sensitive mutant of deoxyribonucleic acid replication of Saccharomyces cerevisiae. Biochemistry. 1973 Oct 23;12(22):4374–4378. doi: 10.1021/bi00746a012. [DOI] [PubMed] [Google Scholar]
  4. Hartwell L. H., Culotti J., Pringle J. R., Reid B. J. Genetic control of the cell division cycle in yeast. Science. 1974 Jan 11;183(4120):46–51. doi: 10.1126/science.183.4120.46. [DOI] [PubMed] [Google Scholar]
  5. Hartwell L. H. Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation. J Mol Biol. 1971 Jul 14;59(1):183–194. doi: 10.1016/0022-2836(71)90420-7. [DOI] [PubMed] [Google Scholar]
  6. Hartwell L. H. Macromolecule synthesis in temperature-sensitive mutants of yeast. J Bacteriol. 1967 May;93(5):1662–1670. doi: 10.1128/jb.93.5.1662-1670.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hartwell L. H. Saccharomyces cerevisiae cell cycle. Bacteriol Rev. 1974 Jun;38(2):164–198. doi: 10.1128/br.38.2.164-198.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartwell L. H. Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. J Bacteriol. 1973 Sep;115(3):966–974. doi: 10.1128/jb.115.3.966-974.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Henson C. P., Perlman P., Weber C. N., Mahler H. R. Formation of yeast mitochondria. II. Effects of antibiotics on enzyme activity during derepression. Biochemistry. 1968 Dec;7(12):4445–4454. doi: 10.1021/bi00852a041. [DOI] [PubMed] [Google Scholar]
  10. Jayaraman J., Cotman C., Mahler H. R., Sharp C. W. Biochemical correlates of respiratory deficiency. VII. Glucose repression. Arch Biochem Biophys. 1966 Sep 26;116(1):224–251. doi: 10.1016/0003-9861(66)90029-4. [DOI] [PubMed] [Google Scholar]
  11. Kim I. C., Beattie D. S. Formation of the yeast mitochondrial membrane. 1. Effects of inhibitors of protein synthesis on the kinetics of enzyme appearance during glucose derepression. Eur J Biochem. 1973 Jul 16;36(2):509–518. doi: 10.1111/j.1432-1033.1973.tb02937.x. [DOI] [PubMed] [Google Scholar]
  12. Linnane A. W., Haslam J. M., Lukins H. B., Nagley P. The biogenesis of mitochondria in microorganisms. Annu Rev Microbiol. 1972;26:163–198. doi: 10.1146/annurev.mi.26.100172.001115. [DOI] [PubMed] [Google Scholar]
  13. Mahler H. R. Biogenetic autonomy of mitochondria. CRC Crit Rev Biochem. 1973 Aug;1(3):381–460. doi: 10.3109/10409237309105439. [DOI] [PubMed] [Google Scholar]
  14. Mahler H. R., Lin C. C. The derepression of delta-aminolevulinate synthetase in yeast. Biochem Biophys Res Commun. 1974 Dec 11;61(3):963–970. doi: 10.1016/0006-291x(74)90249-6. [DOI] [PubMed] [Google Scholar]
  15. Mahler H. R., Perlman P., Henson C., Weber C. Selective effects of chloramphenicol, cycloheximide and nalidixic acid on the biosynthesis of respiratory enzymes in yeast. Biochem Biophys Res Commun. 1968 May 10;31(3):474–480. doi: 10.1016/0006-291x(68)90501-9. [DOI] [PubMed] [Google Scholar]
  16. Perlman P. S., Mahler H. R. Derepression of mitochondria and their enzymes in yeast: regulatory aspects. Arch Biochem Biophys. 1974 May;162(1):248–271. doi: 10.1016/0003-9861(74)90125-8. [DOI] [PubMed] [Google Scholar]
  17. Perlman P. S., Mahler H. R. Intracellular localization of enzymes in yeast. Arch Biochem Biophys. 1970 Jan;136(1):245–259. doi: 10.1016/0003-9861(70)90348-6. [DOI] [PubMed] [Google Scholar]
  18. South D. J., Mahler H. R. RNA synthesis in yeast mitochondria: a derepressible activity. Nature. 1968 Jun 29;218(5148):1226–1232. doi: 10.1038/2181226a0. [DOI] [PubMed] [Google Scholar]
  19. Williamson D. H. The effect of environmental and genetic factors on the replication of mitochondrial DNA in yeast. Symp Soc Exp Biol. 1970;24:247–276. [PubMed] [Google Scholar]
  20. Wintersberger U., Hirsch J., Fink A. M. Studies on nuclear and mitochondrial DNA-replication in a temperature-sensitive mutant of Saccharomyces cerevisiae. Mol Gen Genet. 1974;131(4):291–299. doi: 10.1007/BF00264860. [DOI] [PubMed] [Google Scholar]

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