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. 1975 Sep;123(3):828–836. doi: 10.1128/jb.123.3.828-836.1975

Differences in coupling of energy to glycine and phenylalanine transport in aerobically grown Escherichia coli.

G D Sprott, K Dimock, W G Martin, H Schneider
PMCID: PMC235803  PMID: 1099078

Abstract

Differences exist in the coupling of energy to transport of glycine and phenylalanine in aerobically grown cells of Escherichia coli. Energy derived from respiration, although involved in both uptake systems, is not employed identically as shown by kinetic effects of cyanide and anoxia and by temperature dependencies. Additional evidence for aerobic differences was provided by the effects of azide which greatly decreased initial rates of uptake of glycine but not phenylalanine. The effect on glycine uptake was not due to uncoupling of oxidative phosphorylation or to a decrease in respiration rate. Evidence for anaerobic differences was provided by the addition of either glucose or ferricyanide to cell suspensions containing glycerol, thereby maintaining anoxic uptake of phenylalanine, but not glycine, at the aerobic level. Ferricyanide stimulation required a functional Ca, Mg-adenosine 5'-triphosphatase and involved cell metabolism. Ferricyanide was also found to produce differential stimulation of other amino acid transport systems; tyrosine, tryptophan and leucine uptakes were stimulated whereas those for alanine, proline, threonine, and glutamine were relatively unaffected.

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Selected References

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  1. Bagnara A. S., Finch L. R. Quantitative extraction and estimation of intracellular nucleoside triphosphates of Escherichia coli. Anal Biochem. 1972 Jan;45(1):24–34. doi: 10.1016/0003-2697(72)90004-8. [DOI] [PubMed] [Google Scholar]
  2. Berger E. A. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1514–1518. doi: 10.1073/pnas.70.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger E. A., Heppel L. A. Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli. J Biol Chem. 1974 Dec 25;249(24):7747–7755. [PubMed] [Google Scholar]
  4. Brown K. D. Maintenance and exchange of the aromatic amino acid pool in Escherichia coli. J Bacteriol. 1971 Apr;106(1):70–81. doi: 10.1128/jb.106.1.70-81.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Butlin J. D., Cox G. B., Gibson F. Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem J. 1971 Aug;124(1):75–81. doi: 10.1042/bj1240075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Byard J. L. The effect of beta-galactoside accumulation on the uptake of phosphate into cells and cell nucleotides of Escherichia coli. Biochim Biophys Acta. 1973 Jul 6;311(3):452–461. [PubMed] [Google Scholar]
  7. COHEN G. N., RICKENBERG H. V. Concentration spécifique réversible des amino acides chez Escherichia coli. Ann Inst Pasteur (Paris) 1956 Nov;91(5):693–720. [PubMed] [Google Scholar]
  8. D'Aoust J. Y., Giroux J., Baraan L. R., Schneider H., Martin W. G. Some effects of visible light on Escherichia coli. J Bacteriol. 1974 Nov;120(2):799–804. doi: 10.1128/jb.120.2.799-804.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Devor K. A., Schairer H. U., Renz D., Overath P. Active transport of beta-galactosides by a mutant of Escherichia coli defective in heme synthesis. Eur J Biochem. 1974 Jun 15;45(2):451–456. doi: 10.1111/j.1432-1033.1974.tb03569.x. [DOI] [PubMed] [Google Scholar]
  10. Futai M. Orientation of membrane vesicles from Escherichia coli prepared by different procedures. J Membr Biol. 1974;15(1):15–28. doi: 10.1007/BF01870079. [DOI] [PubMed] [Google Scholar]
  11. Futai M., Sternweis P. C., Heppel L. A. Purification and properties of reconstitutively active and inactive adenosinetriphosphatase from Escherichia coli. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2725–2729. doi: 10.1073/pnas.71.7.2725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HIRSCH C. A., RASMINSKY M., DAVIS B. D., LIN E. C. A FUMARATE REDUCTASE IN ESCHERICHIA COLI DISTINCT FROM SUCCINATE DEHYDROGENASE. J Biol Chem. 1963 Nov;238:3770–3774. [PubMed] [Google Scholar]
  13. Hadjipetrou L., Lilly M. D., Kourounakis P. Effect of ferricyanide on Escherichia coli. Antonie Van Leeuwenhoek. 1970;36(4):531–540. doi: 10.1007/BF02069055. [DOI] [PubMed] [Google Scholar]
  14. Hinds T. R., Brodie A. F. Relationship of a proton gradient to the active transport of proline with membrane vesicles from Mycobacterium phlei. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1202–1206. doi: 10.1073/pnas.71.4.1202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hirata H., Altendorf K., Harold F. M. Energy coupling in membrane vesicles of Escherichia coli. I. Accumulation of metabolites in response to an electrical potential. J Biol Chem. 1974 May 10;249(9):2939–2945. [PubMed] [Google Scholar]
  16. Kaback H. R., Barnes E. M., Jr Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli. J Biol Chem. 1971 Sep 10;246(17):5523–5531. [PubMed] [Google Scholar]
  17. Kaback H. R., Reeves J. P., Short S. A., Lombardi F. J. Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone. Arch Biochem Biophys. 1974 Jan;160(1):215–222. doi: 10.1016/s0003-9861(74)80028-7. [DOI] [PubMed] [Google Scholar]
  18. Kashket E. R., Wilson T. H. Proton-coupled accumulation of galactoside in Streptococcus lactis 7962. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2866–2869. doi: 10.1073/pnas.70.10.2866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Klein W. L., Boyer P. D. Energization of active transport by Escherichia coli. J Biol Chem. 1972 Nov 25;247(22):7257–7265. [PubMed] [Google Scholar]
  20. Kobayashi H., Kin E., Anraku Y. Transport of sugars and amino acids in bacteria. X. Sources of energy and energy coupling reactions of the active transport systems for isoleucine and proline in E. coli. J Biochem. 1974 Aug;76(2):251–261. doi: 10.1093/oxfordjournals.jbchem.a130567. [DOI] [PubMed] [Google Scholar]
  21. Konings W. N., Kaback H. R. Anaerobic transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3376–3381. doi: 10.1073/pnas.70.12.3376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  23. Larsen S. H., Adler J., Gargus J. J., Hogg R. W. Chemomechanical coupling without ATP: the source of energy for motility and chemotaxis in bacteria. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1239–1243. doi: 10.1073/pnas.71.4.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lieberman M. A., Hong J. S. A mutant of Escherichia coli defective in the coupling of metabolic energy to active transport. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4395–4399. doi: 10.1073/pnas.71.11.4395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Nelson N., Kanner B. I., Gutnick D. L. Purification and properties of Mg2+-Ca2+ adenosinetriphosphatase from Escherichia coli. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2720–2724. doi: 10.1073/pnas.71.7.2720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Parnes J. R., Boos W. Energy coupling of the -methylgalactoside transport system of Escherichia coli. J Biol Chem. 1973 Jun 25;248(12):4429–4435. [PubMed] [Google Scholar]
  27. Piperno J. R., Oxender D. L. Amino acid transport systems in Escherichia coli K-12. J Biol Chem. 1968 Nov 25;243(22):5914–5920. [PubMed] [Google Scholar]
  28. Prezioso G., Hong J. S., Kerwar G. K., Kaback H. R. Mechanisms of active transport in isolated bacterial membrane vesicles. XII. Active transport by a mutant of Escherichia coli uncoupled for oxidative phosphorylation. Arch Biochem Biophys. 1973 Feb;154(2):575–582. doi: 10.1016/0003-9861(73)90011-8. [DOI] [PubMed] [Google Scholar]
  29. Rahmanian M., Claus D. R., Oxender D. L. Multiplicity of leucine transport systems in Escherichia coli K-12. J Bacteriol. 1973 Dec;116(3):1258–1266. doi: 10.1128/jb.116.3.1258-1266.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Simoni R. D., Shallenberger M. K. Coupling of energy to active transport of amino acids in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2663–2667. doi: 10.1073/pnas.69.9.2663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Singh A. P., Bragg P. D. Energization of phenylalanine transport and energy-dependent transhydrogenase by ATP in cytochrome-deficient Escherichia coli K12. Biochem Biophys Res Commun. 1974 Apr 23;57(4):1200–1206. doi: 10.1016/0006-291x(74)90824-9. [DOI] [PubMed] [Google Scholar]
  32. Spencer M. E., Guest J. R. Isolation and properties of fumarate reductase mutants of Escherichia coli. J Bacteriol. 1973 May;114(2):563–570. doi: 10.1128/jb.114.2.563-570.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sprott G. D., Dimock K., Martin W. G., Schneider H. Coupling of glycine and alanine transport to respiration in cells of Escherichia coli. Can J Biochem. 1975 Mar;53(3):262–268. doi: 10.1139/o75-037. [DOI] [PubMed] [Google Scholar]
  34. Weiner J. H. The localization of glycerol-3-phosphate dehydrogenase in Escherichia coli. J Membr Biol. 1974;15(1):1–14. doi: 10.1007/BF01870078. [DOI] [PubMed] [Google Scholar]
  35. West I. C., Mitchell P. The proton-translocating ATPase of Escherichia coli. FEBS Lett. 1974 Mar 15;40(1):1–4. doi: 10.1016/0014-5793(74)80880-x. [DOI] [PubMed] [Google Scholar]
  36. Wilson D. B. Source of energy for the Escherichia coli galactose transport systems induced by galactose. J Bacteriol. 1974 Nov;120(2):866–871. doi: 10.1128/jb.120.2.866-871.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Winkler H. H., Wilson T. H. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed] [Google Scholar]
  38. Yamamoto T. H., Mével-Ninio M., Valentine R. C. Essential role of membrane ATPase or coupling factor for anaerobic growth and anaerobic active transport in Escherichia coli. Biochim Biophys Acta. 1973 Sep 26;314(3):267–275. doi: 10.1016/0005-2728(73)90111-4. [DOI] [PubMed] [Google Scholar]
  39. Zwaig N., Kistler W. S., Lin E. C. Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli. J Bacteriol. 1970 Jun;102(3):753–759. doi: 10.1128/jb.102.3.753-759.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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