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. 1975 Sep;123(3):1144–1149. doi: 10.1128/jb.123.3.1144-1149.1975

Localization of acid phosphatase in protoplasts from Saccharomyces cerevisiae.

H J van Rijn, W A Linnemans, P Boer
PMCID: PMC235839  PMID: 239923

Abstract

The localization of acid phosphatase (EC 3.1.3.2) in secreting protoplasts prepared from Saccharomyces cerevisiae is reported for the first time. Using a Gomori technique we were able to show acid phosphatase at those organelles in the protoplasts which are generally involved in the processes of biosynthesis and secretion of glycoproteins in eukaryotic cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer H., Sigarlakie E. Ultrathin frozen sections of yeast cells. J Ultrastruct Res. 1972 Jul;40(1):197–204. doi: 10.1016/s0022-5320(72)80032-7. [DOI] [PubMed] [Google Scholar]
  2. Boer P., Van Rijn H. J., Reinking A., Seryn-Parvé E. P. Biosynthesis of acid phosphatase of baker's yeast. Characterization of a protoplast-bound fraction containing precursors of the exo-enzyme. Biochim Biophys Acta. 1975 Feb 19;377(2):331–342. doi: 10.1016/0005-2744(75)90314-9. [DOI] [PubMed] [Google Scholar]
  3. Cortat M., Matile P., Kopp F. Intracellular localization of mannan synthetase activity in budding baker's yeast. Biochem Biophys Res Commun. 1973 Jul 17;53(2):482–489. doi: 10.1016/0006-291x(73)90687-6. [DOI] [PubMed] [Google Scholar]
  4. Farkas V., Svoboda A., Bauer S. Secretion of cell-wall glycoproteins by yeast protoplasts. Effect of 2-deoxy-D-glucose and cycloheximide. Biochem J. 1970 Aug;118(5):755–758. doi: 10.1042/bj1180755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Günther T., Kattner W., Merker H. J. Uber das Verhalten und die Lokalisation der suaren Phosphatase von Hefezellen bei Repression und Derepression. Exp Cell Res. 1967 Jan;45(1):133–147. doi: 10.1016/0014-4827(67)90118-8. [DOI] [PubMed] [Google Scholar]
  6. Hereward F. V. Rough membranes in Schizosaccharomyces pombe protoplasts. Exp Cell Res. 1974 Jul;87(1):213–218. doi: 10.1016/0014-4827(74)90544-8. [DOI] [PubMed] [Google Scholar]
  7. MCLELLAN W. L., Jr, LAMPEN J. O. The acid phosphatase of yeast. Localization and secretion by protoplasts. Biochim Biophys Acta. 1963 Feb 12;67:324–326. doi: 10.1016/0006-3002(63)91832-8. [DOI] [PubMed] [Google Scholar]
  8. MILLONIG G. A modified procedure for lead staining of thin sections. J Biophys Biochem Cytol. 1961 Dec;11:736–739. doi: 10.1083/jcb.11.3.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moor H. Endoplasmic reticulum as the initiator of bud formation in yeast (S. cerevisiae). Arch Mikrobiol. 1967 Jun 6;57(2):135–146. doi: 10.1007/BF00408697. [DOI] [PubMed] [Google Scholar]
  10. NOVIKOFF A. B., ESSNER E., QUINTANA N. GOLGI APPARATUS AND LYSOSOMES. Fed Proc. 1964 Sep-Oct;23:1010–1022. [PubMed] [Google Scholar]
  11. Roland J. C. The relationship between the plasmalemma and plant cell wall. Int Rev Cytol. 1973;36:45–92. doi: 10.1016/s0074-7696(08)60215-6. [DOI] [PubMed] [Google Scholar]
  12. Van Rijn H. J., Boer P., Steyn-Parvé E. P. Biosynthesis of acid phosphatase of baker's yeast. Factors influencing its production by protoplasts and characterization of the secreted enzyme. Biochim Biophys Acta. 1972 May 12;268(2):431–441. doi: 10.1016/0005-2744(72)90339-7. [DOI] [PubMed] [Google Scholar]

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