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. 1975 Sep;123(3):1150–1156. doi: 10.1128/jb.123.3.1150-1156.1975

Proteinase C (carboxypeptidase Y) mutant of yeast.

D H Wolf, G R Fink
PMCID: PMC235840  PMID: 51020

Abstract

A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of proteinase C does not affect mitotic growth and has no obvious effect on the formation of viable ascospores or meiotic segregation. The mutant grows on peptides known to be cleaved by proteinase C in vitro. This finding is consistent with the idea that other enzymes exist in vivo with overlapping substrate specificities.

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Selected References

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