Abstract
The positional distribution of fatty acids was determined in the major groups of glycerolipids from the mycelium and sporangiophores of the fungus Phycomyces blakesleeanus. At the sn-1 positions of the triacylglycerols, in both regions of the fungus, greater than 65% of the fatty acids were 16:0 and 18:1. At the sn-2 positions of the triacylglycerols, 18:1, 18:2 and 18:3 comprised greater than 85% of the sporangial fatty acids and more than 90% of the mycelial fatty acids. Positions sn-3 of the triacylglycerols, from both regions of the fungus, contained approximately 40% of 16:0, approximately 30% of 18:2, and the largest proportions of 18:3 (21%) in the triacyglycerols. The major phosphoglycerides of P. blakesleeanus mycelium and sporangiophores are phosphatidylcholine and phosphatidylethanolamine, and more than 85% of the fatty acids at the sn-1 positions of these phosphatides consisted of 16:0, 18:2, and 18:3. The sn-2 positions of phosphatidylcholine and phosphatidylethanolamine contained approximately 98% unsaturated fatty acids. In the phosphoglycerides of both regions of the fungus, 18:2 and 18:3 constituted greater than 85% of the total fatty acids. Although the mycelium and sporangiophores of P. blakesleeanus had different morphological and physiological characteristics, the major glycerolipids of the two regions had similar stereospecific distributions of fatty acids.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ahlquist C. N. Phycomyces: Mechanical Behavior of Stage II and Stage IV. Plant Physiol. 1973 Mar;51(3):586–587. doi: 10.1104/pp.51.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bergman K., Burke P. V., Cerdá-Olmedo E., David C. N., Delbrück M., Foster K. W., Goodell E. W., Heisenberg M., Meissner G., Zalokar M. Phycomyces. Bacteriol Rev. 1969 Mar;33(1):99–157. doi: 10.1128/br.33.1.99-157.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brockerhoff H. Determination of the positional distribution of fatty acids in glycerolipids. Methods Enzymol. 1975;35:315–325. doi: 10.1016/0076-6879(75)35171-9. [DOI] [PubMed] [Google Scholar]
- Brockerhoff H. Stereospecific analysis of triglycerides. Lipids. 1971 Dec;6(12):942–956. doi: 10.1007/BF02531179. [DOI] [PubMed] [Google Scholar]
- Brockerhoff H. Stereospecific analysis of triglycerides: an alternative method. J Lipid Res. 1967 May;8(3):167–169. [PubMed] [Google Scholar]
- CASTLE E. S. Growth distribution in the light-growth response of Phycomyces. J Gen Physiol. 1959 Mar 20;42(4):697–702. doi: 10.1085/jgp.42.4.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen R. J. Some properties of chitinase from Phycomyces blakesleeanus. Life Sci. 1974 Jul 15;15(2):289–300. doi: 10.1016/0024-3205(74)90219-7. [DOI] [PubMed] [Google Scholar]
- Foster K. W., Lipson E. D. The light growth response of Phycomyces. J Gen Physiol. 1973 Nov;62(5):590–617. doi: 10.1085/jgp.62.5.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jack R. C. Relation of triglycerides to phosphoglycerides in fungi. J Am Oil Chem Soc. 1965 Dec;42(12):1051–1053. doi: 10.1007/BF02636903. [DOI] [PubMed] [Google Scholar]
- Ortega J. K., Gamow R. I., Ahlquist C. N. Phycomyces: a change in mechanical properties after a light stimulus. Plant Physiol. 1975 Feb;55(2):333–337. doi: 10.1104/pp.55.2.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ortega J. K., Gamow R. I. The problem of handedness reversal during the spiral growth of Phycomyces. J Theor Biol. 1974 Oct;47(2):317–332. doi: 10.1016/0022-5193(74)90201-x. [DOI] [PubMed] [Google Scholar]
- Siakotos A. N. Analytical separation of nonlipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography. J Am Oil Chem Soc. 1965 Nov;42(11):913–919. doi: 10.1007/BF02632444. [DOI] [PubMed] [Google Scholar]
- Skipski V. P., Peterson R. F., Barclay M. Quantitative analysis of phospholipids by thin-layer chromatography. Biochem J. 1964 Feb;90(2):374–378. doi: 10.1042/bj0900374. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yuh-Nung-Jan Properties and cellular localization of chitin synthetase in Phycomyces blakesleeanus. J Biol Chem. 1974 Mar 25;249(6):1973–1979. [PubMed] [Google Scholar]
- Yurkowski M., Brockerhoff H. Fatty acid distribution of triglycerides determined by deacylation with methyl magnesium bromide. Biochim Biophys Acta. 1966 Aug 3;125(1):55–59. doi: 10.1016/0005-2760(66)90143-3. [DOI] [PubMed] [Google Scholar]